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Mechanisms of specific regulation of gonadotropn subunits expression by pulsatile GnRH stimulation

Research Project

Project/Area Number 23592404
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionShimane University

Principal Investigator

KANASAKI Haruhiko  島根大学, 医学部, 講師 (10325053)

Co-Investigator(Kenkyū-buntansha) ORIDE Aki  島根大学, 医学部, 助教 (00423278)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsGnRH / ゴナドトロピン / GnRH / TSA / FSH / PACAP
Research Abstract

PACAP and its receptor are expressed not only in the central nervous system, but also in peripheral organs and function to stimulate pituitary hormone synthesis and release as both hypothalamic-pituitary releasing factor as well as an autocrine-paracrine factor within the pituitary. PACAP stimulates the expression of the gonadotropin alpha, LH betand FSH beta subunits as well as the GnRH receptor and its own PACAP type I receptor (PAC1R) in gonadotropin-secreting pituitary cells. In turn, GnRH, which has been known as a crucial component of gonadotropin secretion, stimulates the expression of PACAP and PAC1R in gonadotrophs. In addition, PAC1R presents in GnRH-producing neuronal cells and PACAP modulates the functions of GnRH neurons. It is evident that PACAP plays a role in reproductive function through regulating gonadotropins directly or indirectly by modulating GnRH release.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (31 results)

All 2013 2012 2011 2010

All Journal Article (22 results) (of which Peer Reviewed: 12 results) Presentation (7 results) Book (2 results)

  • [Journal Article] Expression of gonadotropin-inhibitory hormone receptors in mouse pituitary gonadotroph LβT2 cells and hypothalamic gonadotropin-releasing hormone-producing GT1-7 cells2013

    • Author(s)
      Sukhbaatar U, Kanasaki H, Mijiddorj T, Oride A, Miyazaki K
    • Journal Title

      Endocr J

      Volume: 61 Pages: 253-34

    • NAID

      130004443925

    • Related Report
      2013 Final Research Report
  • [Journal Article] Kisspeptin induces expression of gonadotropin-releasing hormone receptor in GnRH-producing GT1-7 cells overexpressing G protein-coupled receptor 542013

    • Author(s)
      Sukhbaatar U, Kanasaki H, Mijiddorj T, Oride A, Miyazaki K
    • Journal Title

      Gen Comp Endocrinol

      Volume: 194 Pages: 94-101

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Pituitary adenylate cyclase-activating polypeptide (PACAP) increases expression of the gonadotropin-releasing hormone (GnRH) receptor in GnRH-producing GT1-7 cells overexpressiong PACAP type I receptor2013

    • Author(s)
      Kanasaki H, Mijiddorj T, Sukhbaatar U, Oride A, Miyazaki K
    • Journal Title

      Gen Comp Endocrinol

      Volume: 193 Pages: 95-102

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
  • [Journal Article] Circulating kisspeptin and pituitary adenylate cyclase-activating polypeptide (PACAP) do not correlate with gonadotropin serum levels2013

    • Author(s)
      Kanasaki H, Purwana IN, Oride A, Mijiddorj T, Sukhbaatar U, Miyazaki K
    • Journal Title

      Gynecol Endocrinol

      Volume: 29 Pages: 583-587

    • Related Report
      2013 Final Research Report
  • [Journal Article] Possible role of PACAP and its PAC1 receptor in the differential regulation of pituitary LHβ- and FSHβ- subunit gene expression by pulsatile GnRH stimulation2013

    • Author(s)
      Kanasaki H, Purwana IN, Miyazaki K
    • Journal Title

      Biol Reprod

      Volume: 88 Pages: 1-5

    • Related Report
      2013 Final Research Report
  • [Journal Article] Prolonged stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells2013

    • Author(s)
      Mijiddorj T, Kanasaki H, Sukhbaatar U, Oride A, Purwana IN, Miyazaki K
    • Journal Title

      Mol Cell Endocrinol

      Volume: 365 Pages: 139-145

    • Related Report
      2013 Final Research Report
  • [Journal Article] Circulating kisspeptin and pituitary adenylate cyclase-activating polypeptide (PACAP) do not correlate with gonadotropins serum levels2013

    • Author(s)
      Kanasaki H, Purwana IN, Aki Oride, Mijiddorj, U. Sukhbaatar, Miyazaki K
    • Journal Title

      Gynecol Endocrinol

      Volume: 29 Pages: 583-587

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Successful pregnancy after the treatment of primary amenorrhea in a patient with non-classical congenital adrenal hyperplasia: a case report2013

    • Author(s)
      Purwana IN, Kanasaki H, Oride A and Miyazaki K
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 39 Pages: 406-409

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Possible role of PACAP and its PAC1 receptor in the differential regulation of pituitary LH- and FSH- subunit gene expression by pulsatile GnRH stimulation2013

    • Author(s)
      Kanasaki H, Purwana IN, Miyazaki K
    • Journal Title

      Biol Reprod

      Volume: 88 Pages: 1-5

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Gonadotropin-inhibitory hormone inhibits GnRH-induced gonadotropin subunit gene transcriptions by inhibiting AC/cAMP/PKA-dependent ERK pathway in LβT2 cells2012

    • Author(s)
      Son YL, Ubuka T, Millar RP, Kanasaki H, Tsutsui K
    • Journal Title

      Endocrinology

      Volume: 153 Pages: 2332-2343

    • Related Report
      2013 Final Research Report
  • [Journal Article] Role of Neurokinin B and Dynorphin A in pituitary gonadotroph and somatolactotroph cell lines2012

    • Author(s)
      Mijiddorj T, Kanasaki H, Purwana IN, Oride A, Sukhbaatar U and Miyazaki K
    • Journal Title

      Endocr J

      Volume: 59 Pages: 631-640

    • NAID

      10031064434

    • Related Report
      2013 Final Research Report
  • [Journal Article] Effects of estradiol and progesterone on gonadotropin LHβ- and FSHβ-subunit promoter activities in gonadotroph LβT2 cells2012

    • Author(s)
      Kanasaki H, Purwana IN, Mijiddorj T, Sukhbaatar U, Oride A, Miyazaki K
    • Journal Title

      Neuro Endocrinol Lett

      Volume: 33 Pages: 608-613

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Effects of estradiol and progesterone on prolactin transcriptional activity in somatolactotrophic cells2012

    • Author(s)
      0Mijiddorj T, Kanasaki H, Purwana IN, Oride A, Sukhbaatar U and Miyazaki K
    • Journal Title

      Endocr J

      Volume: 59 Pages: 867-879

    • NAID

      10031123132

    • Related Report
      2013 Final Research Report
  • [Journal Article] Extracellular Signal-Regulated Kinase (ERK) Activation and Mitogen-Activated Protein Kinase Phosphatase 1 Induction by Pulsatile Gonadotropin-Releasing Hormone in Pituitary Gonadotrophs.2012

    • Author(s)
      H Kanasaki
    • Journal Title

      Journal of Signal Transduction

      Volume: 2012 Pages: 1-7

    • DOI

      10.1155/2012/198527

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Gonadotropin-inhibitory hormone inhibits gonadotropin-releasing hormone-Induced gonadotropin synthesis mediated by adenylate cyclase/cAMP/PKA-dependent ERK pathway in LβT2 gonadotrope cells2012

    • Author(s)
      Y.L. Son, T. Ubuka, R.P. Millar, H. Kanasaki, K. Tsutsui
    • Journal Title

      Endocrinology

      Volume: 153 Issue: 5 Pages: 2332-2343

    • DOI

      10.1210/en.2011-1904

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Role of Neurokinin B and Dynorphin A in pituitary gonadotroph and somatolactotroph cell lines2012

    • Author(s)
      Mijiddorj T, Kanasaki H, Purwana IN, Oride A, Sukhbaatar U, Miyazaki K
    • Journal Title

      Endocr J.

      Volume: 59 Pages: 631-640

    • NAID

      10031064434

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Effects of estradiol and progesterone on prolactin transcriptional activity in somatolactotrophic cells2012

    • Author(s)
      Mijiddorj T, Kanasaki H, Purwana I, Unurjargal S, Oride A, Miyazaki K
    • Journal Title

      Endocr J.

      Volume: 59 Pages: 867-879

    • NAID

      10031123132

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dual Specificity Phosphatase 1 (DUSP1) by Pulsatile Gonadotropin-Releasing Hormone Stimulation and the Role of Gonadotropin Subunit Expression2011

    • Author(s)
      Purwana IN, Kanasaki H, Oride A, Mijiddorj T Miyazaki K.
    • Journal Title

      Biology of Reproduction

      Volume: 84 Pages: 996-1004

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Possible Involvement of PACAP and PACAP type 1 Receptor in GnRH-Induced FSH -Subunit Gene Expression.2011

    • Author(s)
      Kanasaki H, Purwana IN, Oride A, Mijiddorj T Miyazaki K.
    • Journal Title

      Regulatory Peptides

      Volume: 167 Pages: 227-232

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Paracrine control of gonadotrophs by somatolactotrophs through TRH-induced follistatin production.2011

    • Author(s)
      Kanasaki H, Oride A, Miyazaki K.
    • Journal Title

      The Open Neuroendocrinology Journal

      Volume: 4 Pages: 102-110

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Stimulatory Effect of Pituitary Adenylate-Cyclase Activating Polypeptide (PACAP) and its PACAP Type I Receptor (PAC1R) on Prolactin Synthesis in Rat Pituitary Somatolactotroph GH3 Cells.2011

    • Author(s)
      Mijiddorj T, Kanasaki H, Purwana IN, Oride A and Miyazaki K.
    • Journal Title

      Molecular and Cellular Endocrinology

      Volume: 339 Pages: 172-179

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Expression of the Pituitary Adenylate Cyclase-Activating Polypeptide (PACAP) Type 1 Receptor (PAC1R) Potentiates the Effects of GnRH on Gonadotropin Subunit Gene Expression.2011

    • Author(s)
      Purwana IN, Kanasaki H, Oride A, Mijiddorj T Miyazaki K.
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 410 Pages: 295-300

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Prolonger stimulation with thyrotropin-releasing hormone and pituitary adenylate cyclase-activating polypeptide desensitize their receptor functions in prolactin-producing GH3 cells2013

    • Author(s)
      Haruhiko Kanasaki, Tselmeg Mijiddorj, Unurjargar Sukhbaatar, Aki Oride, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 95^<th> annual meeting
    • Place of Presentation
      San Francisco, CA(USA)
    • Year and Date
      2013-06-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Stimulatory effect of pituitary adenylate cyclase-activating polypeptide (PACAP) and its PACAP type I receptor (PAC1R) on prolactin synthesis in rat pituitary somatolactotroph GH3 cells2013

    • Author(s)
      Tselmeg Mijiddorj, Haruhiko Kanasaki, Unurjargar Sukhbaatar, Aki Oride, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 95^<th> annual meeting
    • Place of Presentation
      San Francisco, CA(USA)
    • Year and Date
      2013-06-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Expression of the pituitary adenylate cyclase-activating polypeptide (PACAP) type 1 receptor (PAC1R) potentiates the effects of GnRH on gonadotropin subunit gene expression2012

    • Author(s)
      Haruhiko Kanasaki, Aki Oride, Indri Purwana, Tselmeg Mijiddorj, Unurjargal Sukhbaatar, Kohji Miyazaki
    • Organizer
      The Endocrine Society's 94^<th> annual meeting
    • Place of Presentation
      Houston, TX (USA)
    • Year and Date
      2012-06-26
    • Related Report
      2013 Final Research Report
  • [Presentation] Circulating kisspeptin and pituitary adenylate cyclase-activating polypeptide do not correlate with gonadotropin serum levels2012

    • Author(s)
      Haruhiko Kanasaki, Aki Oride, Tselmeg Mijiddorj, Unurjargar Sukhbaatar, Kohji Miyazaki
    • Organizer
      2nd World Conferance Kisspeptin
    • Place of Presentation
      Tokyo
    • Related Report
      2013 Final Research Report
  • [Presentation] GnRH-induced PACAP and PAC1 Receptor Expression in Pituitary Gonadotrophs : A Possible Role in the Regulation of Gonadotropin Subunit Gene Expression2011

    • Author(s)
      Kanasaki H, Oride A, Purwana IN Mijiddorj T and Miyazaki K
    • Organizer
      The Endocrine Society's 93^<rd> annual meeting
    • Place of Presentation
      Boston, MA (USA)
    • Year and Date
      2011-06-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 卵子の成熟に果たす下垂体LH, FSH産生の意義2011

    • Author(s)
      金崎春彦、プルワナ インドリ、折出亜希、ミジドルジ ツェルメグ、宮崎康二
    • Organizer
      第29回日本受精着床学会学術講演会 シンポジウム
    • Place of Presentation
      京王プラザホテル(東京)
    • Related Report
      2011 Research-status Report
  • [Presentation] Follistatin, induced by thyrotropin-releasing hormone (TRH), plays no role in prolactin expression but affects gonadotropin FSHb expression as a paracrine factor in pituitary somatolactotroph GH3 cells2010

    • Author(s)
      Kanasaki H, Oride A, Purwana IN, Miyazaki K
    • Organizer
      14^<th> International Congress of Endocrinology (ICE2010)
    • Place of Presentation
      Kyoto(Japan)
    • Related Report
      2013 Final Research Report
  • [Book] Specific regulation of gonadotropin LHβ- and FSHβ-subunit gene expression by pulsatile GnRH stimulation. Gonadotropin-releasing hormone (GnRH): Production, Structure & Function2013

    • Author(s)
      Haruhiko Kanasaki, Aki Oride Kohji Miyazaki
    • Publisher
      Nova Scientific Press, Inc (Ney York)
    • Related Report
      2013 Final Research Report
  • [Book] Gonadotropin-releasing hormone (GnRH): Production, Structure & Function2013

    • Author(s)
      Haruhiko Kanasaki, Aki Oride Kohji Miyazaki
    • Total Pages
      8
    • Publisher
      Nova Scientific Press, Inc
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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