Nuclear matrix associating factors, Satb, are novel regulators of murine placentation

• Project/Area Number: 23592405
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Kyushu University
• Principal Investigator: ASANOMA Kazuo 九州大学, 大学病院, 助教 (30380413)
• Co-Investigator(Kenkyū-buntansha): FUKUSHIMA Kotaro 九州大学病院, 産科婦人科, 講師 (40304779)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 胎盤 / 栄養膜幹細胞 / 分化 / 転写因子 / 転写調節 / 国際情報交換 / 胎盤栄養膜幹細胞 / 転写調節機構

Research Abstract

We identified novel regulators of trophoblast cell differentiation, Satb1 and Satb2. They had been known as nuclear matrix associating molecules. Satb1 and Satb2 are necessary to maintain rodent trophoblast cells at stem cell condition. We also discovered Eomes as a target gene of Satb1 and Satb2. Eomes is a well-known regulator necessary for maintenance of trophoblast stem cells. Therefore, Satb1 and Satb2 upregulate Eomes expression to keep trophoblast cells from differentiation. We published these results as a paper (Asanoma et al., 2012).

Research Products

• [Journal Article] Inhibition of AHR transcription by NF1C is affected by a single nucleotide polymorphism, and is involved in suppression of human uterine endometrial cancer (2013)
  • Author(s): Li D, Takao T, Tsunematsu R, Morokuma S, Fukushima K, Kobayashi H, Saito T, Furue M, Wake N, Asanoma K
  • Journal Title: Oncogene Volume: 32 Pages: 4950-4959
• [Journal Article] Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody (2013)
  • Author(s): Wake N, Takao T, Asanoma K, Kato H
  • Journal Title: Journal of Obstetic and Gynaecology Res Volume: 39 Pages: 1230-1235
• [Journal Article] Establishment of a new diagnostic method for hydropic villi by using TSSC3 antibody. (2013)
  • Author(s): Wake N, Takao T, Asanoma K, Kato H
  • Journal Title: J Obstet Gynaecol Res. Volume: 39(7) Issue: 7 Pages: 1230-1235
  • DOI: 10.1111/jog.12100
  • Peer Reviewed
• [Journal Article] SATB homeobox proteins regulate trophoblast stem cell renewal and differentiation (2012)
  • Author(s): Asanoma K, Kubota K, Chakraborty D, Renaud SJ, Wake N, Fukushima K, Soares MJ, Rumi MA
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 2257-2268
• [Journal Article] The maternally expressed gene Tssc3 regulates the expression of MASH2 transcription factor in mouse trophoblast stem cells through the AKT-Sp1 signaling pathway (2012)
  • Author(s): Takao T, Asanoma K, Tsunematsu R, Kato K, Wake N
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 42685-42694
• [Journal Article] Inhibition of AHR transcription by NF1C is affected by a single-nucleotide polymorphism, and is involved in suppression of human uterine endometrial cancer. (2012)
  • Author(s): Li D, Takao T, Tsunematsu R, Morokuma S, Fukushima K, Kobayashi H, Saito T, Furue M, Wake N, Asanoma K
  • Journal Title: Oncogene Volume: in press Issue: 41 Pages: 4950-4959
  • DOI: 10.1038/onc.2012.509
  • Peer Reviewed
• [Journal Article] The maternally expressed gene Tssc3 regulates the expression of MASH2 transcription factor in mouse trophoblast stem cells through the AKT-Sp1 signaling pathway. (2012)
  • Author(s): Takao T, Asanoma K, Tsunematsu R, Kato K, Wake N.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Issue: 51 Pages: 42685-42694
  • DOI: 10.1074/jbc.m112.388777
  • Peer Reviewed
• [Journal Article] SATB homeobox proteins regulate trophoblast stem cell renewal and differentiation. (2012)
  • Author(s): Asanoma K, Kubota K, Chakraborty D, Renaud SJ, Wake N, Fukushima K, Soares MJ, Rumi MA.
  • Journal Title: Journal of Biological Chemistry Volume: 287 Pages: 2257-2268
  • Peer Reviewed
• [Journal Article] Chromosome-substituted rat strains provide insights into the genetics of placentation (2011)
  • Author(s): Konno T, Rempel LA, Rumi MA, Graham AR, Asanoma K, Renaud SJ, Soares MJ
  • Journal Title: Physiolocgical Genomics Volume: 43 Pages: 930-941
• [Journal Article] Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line (2011)
  • Author(s): Takao T, Asanoma K, Kato K, Fukushima K, Tsunematsu R, Hirakawa T, Matsumura S, Seki H, Takeda S, Wake N
  • Journal Title: PLosOne Volume: 6
• [Journal Article] Chromosome-substituted rat strains provide insights into the genetics of placentation. (2011)
  • Author(s): Konno T, Rempel LA, Rumi MA, Graham AR, Asanoma K, Renaud SJ, Soares MJ.
  • Journal Title: Physiological Genomics Volume: 43 Pages: 930-941
  • Peer Reviewed
• [Journal Article] Isolation and characterization of human trophoblast side-population (SP) cells in primary villous cytotrophoblasts and HTR-8/SVneo cell line. (2011)
  • Author(s): Takao T, Asanoma K, Kato K, Fukushima K, Tsunematsu R, Hirakawa T, Matsumura S, Seki H, Takeda S, Wake N.
  • Journal Title: PLos One Volume: 6
  • Peer Reviewed
• [Presentation] ダイオキシン受容体は一塩基多型依存的に転写制御され、子宮体癌の増殖・浸潤に関わる (2013)
  • Author(s): 淺野間 和夫, 髙尾 知佳, 恒松 良祐, 諸隈 誠一, 福嶋恒太郎, 小林 裕明, 齋藤俊章, 加藤 聖子, 和氣徳夫
  • Organizer: 第54回日本婦人科腫瘍学会学術講演会
  • Year and Date: 2013-07-19
• [Presentation] ダイオキシン受容体は一塩基多型依存的に転写制御され、子宮体癌の増殖・浸潤に関わる (2013)
  • Author(s): 淺野間 和夫, 髙尾 知佳, 恒松 良祐, 諸隈 誠一, 福嶋恒太郎, 小林 裕明, 齋藤俊章, 加藤 聖子, 和氣徳夫
  • Organizer: 第12回に本婦人科がん分子標的研究会学術集会
  • Year and Date: 2013-07-06
• [Presentation] 転写因子NF1C は一塩基多型依存的にダイオキシン受容体の転写を抑制し、子宮内膜癌の増殖・浸潤を制御する (2013)
  • Author(s): 淺野間 和夫, 恒松 良祐, 諸隈 誠一, 福嶋 恒太郎, 小林 裕明, 齋藤 俊章, 加藤 聖子, 和氣 徳夫
  • Organizer: 第65回日本産婦人科学会学術講演会
  • Year and Date: 2013-05-10
• [Presentation] Regulation of trophoblast differentiation by nuclear matrix associated gene, Satb1 and Satb2 (2012)
  • Author(s): Kazuo Asanoma, Kaiyu Kubota, Damayanti Chakraborty, Stephen J. Renaud, Norio Wake, Kotaro Fukushima, Michael J. Soares, M. A. Karim Rumi
  • Organizer: International Federation of Placenta Association Meeting 2012
  • Year and Date: 2012-09-18
• [Presentation] マウス栄養膜細胞の分化を制御する新たな分子、核マトリックス関連分子Satb1, Satb2 の解析 (2012)
  • Author(s): 淺野間和夫、福嶋恒太郎、和氣徳夫
  • Organizer: 日本産科婦人科学会第64回学術講演会
  • Year and Date: 2012-04-15
• [Presentation] Establishment of trophoblast cell lines from rat blastocysts possessing the capacity to differentiate into multiple trophoblast cell lineage (2011)
  • Author(s): Asanoma Kazuo、Wake Noio
  • Organizer: 63th Annual Congress of the Japan Society of Obstetrics and Gynecology
  • Year and Date: 2011-08-29
• [Presentation] Establishment of trophoblast cell lines from rat blastocysts (2011)
  • Author(s): Asanoma Kazuo、Wake Noio
  • Organizer: 63th Annual Congress of the Japan Society of Obstetrics and Gynecology
  • Place of Presentation: Osaka
• [Presentation] 転写因子NF1Cは一塩基多型依存的にダイオキシン受容体の転写を抑制し、子宮内膜癌の増殖・浸潤を制御する
  • Author(s): 淺野間 和夫, 恒松 良祐, 諸隈 誠一, 福嶋 恒太郎, 小林 裕明, 齋藤 俊章, 加藤 聖子, 和氣 徳夫
  • Organizer: 第65回日本産婦人科学会学術講演会
  • Place of Presentation: 札幌市
• [Presentation] ダイオキシン受容体は一塩基多型依存的に転写制御され、子宮体癌の増殖・浸潤に関わる
  • Author(s): 淺野間 和夫, 髙尾 知佳, 恒松 良祐, 諸隈 誠一, 福嶋恒太郎, 小林 裕明, 齋藤俊章, 加藤 聖子, 和氣徳夫
  • Organizer: 第12回に本婦人科がん分子標的研究会学術集会
  • Place of Presentation: 奈良市
• [Presentation] ダイオキシン受容体は一塩基多型依存的に転写制御され、子宮体癌の増殖・浸潤に関わる
  • Author(s): 淺野間 和夫, 髙尾 知佳, 恒松 良祐, 諸隈 誠一, 福嶋恒太郎, 小林 裕明, 齋藤俊章, 加藤 聖子, 和氣徳夫
  • Organizer: 第54回日本婦人科腫瘍学会学術講演会
  • Place of Presentation: 東京
• [Presentation] マウス栄養膜細胞の分化を制御する新たな分子、核マトリックス関連分子Satb1, Satb2の解析
  • Author(s): 浅野間和夫、福嶋恒太郎、和氣徳夫
  • Organizer: 日本産科婦人科学会第６４回学術講演会
  • Place of Presentation: 神戸
• [Presentation] Regulation of trophoblast differentiation by nuclear matrix associated gene, Satb1 and Satb2
  • Author(s): Kazuo Asanoma, Kaiyu Kubota, Damayanti Chakraborty, Stephen J. Renaud, Norio Wake, Kotaro Fukushima, Michael J. Soares, M. A. Karim Rumi
  • Organizer: International Federation of Placenta Association Meeting
  • Place of Presentation: Hiroshima