| Project/Area Number |
23592414
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Keio University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
HITOSHI Ishimoto 東海大学, 医学部, 教授 (10212937)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 羊膜上皮細胞 / マトリクス細胞蛋白 / 羊膜間葉細胞 / 網羅的遺伝子発現解析 / matricellular protein / amnion / prostaglandin / SPARC / TSP1 / TSP2 |
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| Outline of Final Research Achievements |
(1) Expression changes of matrix cellular proteins of the amniotic membrane in mid and late gestation: the expression of SPARC mRNA was reduced by 40% at the late gestation. Expression of TSP1 was increased three-fold in late gestation. (2) Expression changes of prostaglandin synthesis enzyme: only COX2 mRNA expression was increased twice at the late gestation. (3) Establishment of amniotic epithelial cell separation method using flow cytometry: because there was instability in the cell growth of the original amniotic epithelial cells, it was forced to change the experimental plan. A new separation method was established. (4) Comprehensive gene expression analysis in epithelial cells obtained by flow cytometry: in primary epithelial cells, the expression of midkine and FGF2 involved in the proliferation was low, the expression of HLA-G related to immunosuppression was higher. After passage, the expression of midkine and FGF2 was increased rapidly, HLA-G expression was decreased.
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