Development of the method of selecting the sperm in the ART (Assisted reproductive technology)

Research Project

Project/Area Number	23592420
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Obstetrics and gynecology
Research Institution	Okinawa Institute of Science and Technology Graduate University
Principal Investigator	SUGIYAMA Hitoshi 沖縄科学技術大学院大学, サイエンス・テクノロジー・グループ, リサーチサイエンティスト (70301596)
Co-Investigator(Kenkyū-buntansha)	FUNABASHI Toshiya 聖マリアンナ医科大学, 医学部, 教授 (70229102) YAGI Chiaki 琉球大学, 医学部付属病院, 助教 (20529341) ASATO Kozue 琉球大学, 医学部付属病院, 助教 (30452246)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000) Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords	生殖医療 / 精子 / 走化性 / 受精 / 生殖補助医療 / 走流性 / DNA断片化 / 卵外皮
Research Abstract	In in vitro fertilization using intracytoplasmic sperm injection (ICSI), a single sperm is injected directly into an egg. Although the physiological separation acts in the natural pregnancy, a sperm provided to ICSI is chosen by an embryologist. We customized method of ICSI and some methods for analyses of the sperm DNA fragmentation in mice to perform in our laboratory, and then it was examined whether chemotaxis could use for mammalian sperm separation in this study, but it could not detect the chemotaxis by methods that we used.