| Project/Area Number |
23592420
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Okinawa Institute of Science and Technology Graduate University |
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Principal Investigator |
SUGIYAMA Hitoshi 沖縄科学技術大学院大学, サイエンス・テクノロジー・グループ, リサーチサイエンティスト (70301596)
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| Co-Investigator(Kenkyū-buntansha) |
FUNABASHI Toshiya 聖マリアンナ医科大学, 医学部, 教授 (70229102)
YAGI Chiaki 琉球大学, 医学部付属病院, 助教 (20529341)
ASATO Kozue 琉球大学, 医学部付属病院, 助教 (30452246)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 生殖医療 / 精子 / 走化性 / 受精 / 生殖補助医療 / 走流性 / DNA断片化 / 卵外皮 |
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| Research Abstract |
In in vitro fertilization using intracytoplasmic sperm injection (ICSI), a single sperm is injected directly into an egg. Although the physiological separation acts in the natural pregnancy, a sperm provided to ICSI is chosen by an embryologist. We customized method of ICSI and some methods for analyses of the sperm DNA fragmentation in mice to perform in our laboratory, and then it was examined whether chemotaxis could use for mammalian sperm separation in this study, but it could not detect the chemotaxis by methods that we used.
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