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Screening of the specific gene of invasive moles

Research Project

Project/Area Number 23592435
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionChiba University

Principal Investigator

USUI Hirokazu  千葉大学, 医学(系)研究科(研究院), 講師 (90375634)

Co-Investigator(Renkei-kenkyūsha) SHOZU Makio  千葉大学, 大学院医学研究院, 教授 (30226302)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords侵入奇胎 / 雄核発生奇胎 / トロホブラスト / 絨毛外栄養膜細胞 / 低酸素 / インプリント遺伝子 / p57KIP2 / マイクロアレイ
Research Abstract

We planned to identify the specific gene of invasive mole development, compared the expression pattern of primary culture cell of extravillous trophoblasts between the case of gestational trophoblastic neoplasia (GTN) development and the case primary remission. We purified 15 cases of EVTs cells. Among them, only one case developed to GTN. The case that became the invasive mole was not enough for the original purpose.
We compared the expression of key genes in the villus differentiation between the purified EVTs and uncultured villous tissue. In addition, we confirmed a methylation status of the imprinted gene, KvDMR, which controlled the expression of p57KIP2. The increased expression level of p57KIP2 was regulated by the possible mechanism except for the methylation of imprinted gene, KvDMR.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (50 results)

All 2014 2013 2012 2011 Other

All Journal Article (21 results) (of which Peer Reviewed: 16 results) Presentation (27 results) (of which Invited: 4 results) Remarks (2 results)

  • [Journal Article] Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion by targeting focal adhesion pathways in cervical squamous cell carcinoma2013

    • Author(s)
      Yamamoto N, Kinoshita T, Nohata N, Itesako T, Yoshino H, Enokida H, Nakagawa M, Shozu M, and Seki N
    • Journal Title

      International journal of oncology

      Volume: 42 Issue: 5 Pages: 1523-1532

    • DOI

      10.3892/ijo.2013.1851

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Numerous psammoma bodies in cervical cytology of high-grade endocervical adenocarcinoma.2013

    • Author(s)
      Uehara T, Kiyokawa T, Tate S, Usui H, and Shozu M
    • Journal Title

      Cytopathology

      Volume: - Issue: 4 Pages: 274-275

    • DOI

      10.1111/cyt.12080

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 婦人科悪性腫瘍と妊孕能温存(その2)絨毛性疾患2013

    • Author(s)
      碓井宏和
    • Journal Title

      日本婦人科腫瘍学会雑誌

      Volume: 31 Pages: 1006-1011

    • URL

      http://search.jamas.or.jp/link/ui/NC11130410

    • Related Report
      2013 Final Research Report
  • [Journal Article] Tumor-suppressive microRNA-29a inhibits cancer cell migration and invasion via targeting HSP47 in cervical squamous cell carcinoma.2013

    • Author(s)
      Yamamoto N, Kinoshita T, Nohata N, Yoshino H, Itesako T, Fujimura L, Mitsuhashi A, Usui H, Enokida H, Nakagawa M, Shozu M, and Seki N
    • Journal Title

      Int J Oncol

      Volume: 43 Issue: 6 Pages: 1855-1863

    • DOI

      10.3892/ijo.2013.2145

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 婦人科悪性腫瘍と妊孕能温存(その2) 絨毛性疾患2013

    • Author(s)
      碓井宏和
    • Journal Title

      日本婦人科腫瘍学会雑誌

      Volume: 31 Pages: 1006-1011

    • Related Report
      2013 Annual Research Report
  • [Journal Article] Daily Low-Dose Cisplatin-Based Concurrent Chemoradiotherapy in Patients With Uterine Cervical Cancer With Emphasis on Elderly Patients: A Phase 2 Trial2012

    • Author(s)
      Mitsuhashi A, Uno T, Usui H, Nishikimi K, Yamamoto N, Watanabe M, et al.
    • Journal Title

      Int J Gynecol Cancer

      Volume: - Issue: 8 Pages: 1453-1458

    • DOI

      10.1097/igc.0b013e3182559bda

    • Related Report
      2013 Annual Research Report 2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Normal human chorionic gonadotropin regression curves in uneventful postmolar patients2012

    • Author(s)
      Kizaki S, Matsui H, Usui H, Shozu M, Hanawa S, Yamamoto E, and Kikkawa F
    • Journal Title

      J Reprod Med

      Volume: 57 Pages: 243-248

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22696820

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Complicating preeclampsia as a predictor of poor survival of the fetus in complete hydatidiform mole coexistent with twin fetus2012

    • Author(s)
      Kihara M, Usui H, Tanaka H, Inoue H, Matsui H, and Shozu M
    • Journal Title

      J Reprod Med

      Volume: 57 Pages: 325-328

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22838249

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of Angiotensin II receptor-like 1 (APJ) in the Placentas of Pregnancy-Induced Hypertension.2012

    • Author(s)
      Furuya M, Okuda M, Usui H, Takenouchi T, Kami D, Nozawa A, Shozu M, Umezawa A, Takahashi T, Aoki I.
    • Journal Title

      Int Gynecol Pathol

      Volume: 31 Issue: 3 Pages: 227-235

    • DOI

      10.1097/pgp.0b013e31823b6e71

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 腫瘍症例に学ぶ非順調型の臨床経過を辿る絨毛性疾患低単位hCGの取扱いについて2012

    • Author(s)
      碓井宏和
    • Journal Title

      日本産科婦人科学会雑誌

      Volume: 64 Pages: 290-294

    • URL

      http://search.jamas.or.jp/link/ui/2012337635

    • Related Report
      2013 Final Research Report
  • [Journal Article] 【婦人科がん-最新の研究動向-】絨毛性疾患胞状奇胎の管理方針2012

    • Author(s)
      碓井宏和,生水真紀夫
    • Journal Title

      日本臨床

      Volume: 70 Pages: 716-720

    • URL

      http://search.jamas.or.jp/link/ui/2012258671

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Normal human chorionic gonadotropin regression curves in uneventful postmolar patients2012

    • Author(s)
      Kizaki S, Matsui H, Usui H, Shozu M, Hanawa S, Yamamoto E, et al.
    • Journal Title

      J Reprod Med

      Volume: 57 Pages: 243-8

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Complicating preeclampsia as a predictor of poor survival of the fetus in complete hydatidiform mole coexistent with twin fetus2012

    • Author(s)
      Kihara M, Usui H, Tanaka H, Inoue H, Matsui H, Shozu M.
    • Journal Title

      J Reprod Med

      Volume: 57 Pages: 325-8

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 【婦人科がん-最新の研究動向-】 絨毛性疾患 胞状奇胎の管理方針2012

    • Author(s)
      碓井宏和, 生水真紀夫
    • Journal Title

      日本臨床

      Volume: 70 Pages: 716-20

    • Related Report
      2012 Research-status Report
  • [Journal Article] 腫瘍 症例に学ぶ 非順調型の臨床経過を辿る絨毛性疾患 低単位hCGの取扱いについて2012

    • Author(s)
      碓井宏和
    • Journal Title

      日本産科婦人科学会雑誌

      Volume: 64

    • Related Report
      2012 Research-status Report
  • [Journal Article] Molecular distinction of consecutive molar pregnancies2011

    • Author(s)
      Usui H, Kaku H, Kihara M, and Shozu M
    • Journal Title

      Obstet Gynecol

      Volume: 117 Issue: 2 Pages: 492-495

    • DOI

      10.1097/aog.0b013e318205636b

    • Related Report
      2013 Final Research Report 2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] The application of a newly developed linear stapler preloaded with tissue reinforcement for distal pancreatectomy in the management of ovarian cancer2011

    • Author(s)
      Kato K, Tate S, Uehara T, Usui H, Mitsuhashi A, and Shozu M
    • Journal Title

      European journal of gynaecological oncology

      Volume: 32 Pages: 579-581

    • URL

      http://www.ncbi.nlm.nih.gov/pubmed/22053681

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 【婦人科がん妊孕性・卵巣機能温存治療と妊娠・分娩】胞状奇胎後の妊娠2011

    • Author(s)
      碓井宏和,中島名律子,木原真紀,加来博志,生水真紀夫
    • Journal Title

      産婦人科の実際

      Volume: 60 Pages: 217-221

    • URL

      http://search.jamas.or.jp/link/ui/2011116411

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] The application of a newly developed linear stapler preloaded with tissue reinforcement for distal pancreatectomy in the management of ovarian cancer.2011

    • Author(s)
      Kato K, Tate S, Uehara T, Usui H, Mitsuhashi A, Shozu M.
    • Journal Title

      Eur J Gynaecol Oncol

      Volume: 32 Pages: 579-581

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 【婦人科がん妊孕性・卵巣機能温存治療と妊娠・分娩】 胞状奇胎後の妊娠2011

    • Author(s)
      碓井宏和, 中島名律子, 木原真紀, 加来博志, 生水真紀夫
    • Journal Title

      産婦人科の実際

      Volume: 60 Pages: 217-221

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] 【不妊診療のすべて】 総論 卵巣とアロマターゼ.2011

    • Author(s)
      生水真紀夫, 碓井宏和
    • Journal Title

      産婦人科治療

      Volume: 102 Pages: 466-474

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 専攻医教育プログラム 絨毛性疾患2014

    • Author(s)
      碓井宏和
    • Organizer
      第66 回日本産科婦人科学会学術講演会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] SNPアレイ解析による雄核発生2014

    • Author(s)
      碓井宏和, 瞿佳, 前原佳代子, 塙真輔, 山本憲子, 錦見恭子, 植原貴史, 楯真一, 三橋暁, 秦健一郎, 生水真紀夫
    • Organizer
      第66 回日本産科婦人科学会学術講演会
    • Place of Presentation
      東京国際フォーラム
    • Related Report
      2013 Annual Research Report
  • [Presentation] Risk factors for gestational trophoblastic neoplasia development following androgenetic complete hydatidiform mole2013

    • Author(s)
      Hirokazu Usui,Jia Qu,Makio Shozu
    • Organizer
      17th World Congress on Gestational Trophoblastic Diseases
    • Place of Presentation
      Chicago, USA
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 核発生胞状奇胎からの続発症発生リスク因子の検討会2013

    • Author(s)
      碓井宏和,瞿佳,山本憲子,錦見恭子,楯真一,三橋暁,生水真紀夫雄
    • Organizer
      第54回日本婦人科腫瘍学会学術講演
    • Place of Presentation
      東京
    • Year and Date
      2013-07-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 胞状奇胎が疑われた絨毛の肉眼診断・組織診断とDNA診断の比較検討2013

    • Author(s)
      碓井宏和,瞿佳,清川貴子,山本憲子,錦見恭子,楯真一,三橋暁,中谷行雄,生水真紀夫
    • Organizer
      第65回日本産科婦人科学会学術講演会
    • Place of Presentation
      札幌
    • Year and Date
      2013-05-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 胞状奇胎が疑われた絨毛の肉眼診断・組織診断とDNA診断の比較検討2013

    • Author(s)
      碓井宏和, 瞿佳, 清川貴子, 山本憲子, 錦見恭子, 楯真一, 三橋暁, 中谷行雄, 生水真紀夫
    • Organizer
      第65 回日本産科婦人科学会学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 雄核発生胞状奇胎からの 続発症発生リスク因子の検討2013

    • Author(s)
      碓井宏和, 瞿佳, 山本憲子, 錦見恭子, 楯真一, 三橋暁, 生水真紀夫
    • Organizer
      第54 回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      東京(ホテルグランパシフィックLE DAIBA)
    • Related Report
      2013 Annual Research Report
  • [Presentation] STR多型解析による卵巣奇形腫の発生起源決定2013

    • Author(s)
      加来博志, 碓井宏和, 瞿佳,生水真紀夫
    • Organizer
      第65 回日本産科婦人科学会学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] Total hCG(IU/ml)はfree hCG-βサブユニット(ng/ml)に比べて残存絨毛の検出感度が高いか 同時測定128検体の比較検討2013

    • Author(s)
      三木谷敦子, 碓井宏和, 生水真紀夫
    • Organizer
      第65 回日本産科婦人科学会学術講演会
    • Place of Presentation
      札幌
    • Related Report
      2013 Annual Research Report
  • [Presentation] 絨毛性腫瘍に対する手術療法について2013

    • Author(s)
      碓井宏和
    • Organizer
      第31回日本絨毛性疾患研究会
    • Place of Presentation
      名古屋(ウインク愛知)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 婦人科悪性腫瘍と妊孕能温存その2絨毛性疾患2012

    • Author(s)
      碓井宏和
    • Organizer
      第53回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      岡山
    • Year and Date
      2012-11-23
    • Related Report
      2013 Final Research Report
  • [Presentation] The polymorphism of folate metabolic enzyme genes may predict the outcome of methotrexate therapy for low-risk gestational trophoblastic2012

    • Author(s)
      Hirokazu Usui, Jia Qu, Makio Shozu
    • Organizer
      neoplasia IFPA Meeting 2012
    • Place of Presentation
      Hiroshima, Japan
    • Year and Date
      2012-09-19
    • Related Report
      2013 Final Research Report
  • [Presentation] 絨毛性腫瘍に対する化学療法の卵巣機能への影響についての検討2012

    • Author(s)
      碓井宏和,木原真紀,山本憲子,錦見恭子,楯真一,三橋暁,生水真紀夫
    • Organizer
      第52回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      東京
    • Year and Date
      2012-07-20
    • Related Report
      2013 Final Research Report
  • [Presentation] 症例に学ぶ非順調型の臨床経過を辿る絨毛性疾患低単位hCGの取扱いについて2012

    • Author(s)
      碓井宏和
    • Organizer
      第64回日本産科婦人科学会学術講演会
    • Place of Presentation
      神戸
    • Year and Date
      2012-04-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Low-risk gestational trophoblastic neoplasiaに対するMTX療法の効果・副作用と葉酸代謝酵素遺伝子多型の関連絨毛性腫瘍の個別化医療を目指して2012

    • Author(s)
      碓井宏和,木原真紀,加来博志,山本憲子,錦見恭子,加藤一喜,楯真一,三橋暁,生水真紀夫
    • Organizer
      第64回日本産科婦人科学会学術講演会
    • Place of Presentation
      神戸
    • Year and Date
      2012-04-14
    • Related Report
      2013 Final Research Report
  • [Presentation] MANAGEMENT AND OUTCOME OF WOMEN WITH GESTATIONAL TROPHOBLASTIC NEOPLASIA WITHOUT A CLINICALLY DETECTABLE TUMOR2011

    • Author(s)
      Hirokazu Usui, Maki Kihara, Hiroshi Kaku, Qu Jia, Hideo Matsui, Makio Shozu
    • Organizer
      World Congress on Gestational Trophoblastic Diseases
    • Place of Presentation
      Budapest, Hungary
    • Year and Date
      2011-10-17
    • Related Report
      2013 Final Research Report
  • [Presentation] C677T polymorphisms of invasive mole predict the effect of methotrexate therapy2011

    • Author(s)
      Hirokazu Usui, Jia Qu, Akira Mitsuhashi, Makio Shozu
    • Organizer
      第70回日本癌学会学術総会
    • Place of Presentation
      名古屋
    • Year and Date
      2011-10-03
    • Related Report
      2013 Final Research Report
  • [Presentation] FIGO 2000 systemについて2011

    • Author(s)
      碓井宏和
    • Organizer
      第29回絨毛性疾患研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-30
    • Related Report
      2013 Final Research Report
  • [Presentation] Quiescent GTDと考えられた2症例2011

    • Author(s)
      碓井宏和,木原真紀,加来博志,錦見恭子,山本憲子、植原貴史,加藤一喜,楯真一,三橋暁,生水真紀夫
    • Organizer
      第29回絨毛性疾患研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-09-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 核発生奇胎の推定に血清hCG値は有用か2011

    • Author(s)
      碓井宏和、生水真紀夫雄
    • Organizer
      第12回ホルモンと癌研究会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-15
    • Related Report
      2013 Final Research Report
  • [Presentation] Quiescent gestational trophoblastic diseaseと考えられた2症例2011

    • Author(s)
      碓井宏和,木原真紀,加来博志,錦見恭子,山本憲子、植原貴史,加藤一喜,楯真一,三橋暁,生水真紀夫
    • Organizer
      第121回関東連合産科婦人科学会学術集会
    • Place of Presentation
      東京
    • Year and Date
      2011-06-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 絨毛性腫瘍に対するメトトレキサート療法の有害事象の検討2011

    • Author(s)
      碓井宏和, 木原真紀, 加来博志, 山本憲子, 錦見恭子, 植原貴史, 加藤一喜, 楯真一, 三橋暁, 生水真紀夫
    • Organizer
      第63回日本産科婦人科学会学術講演会
    • Place of Presentation
      リーガロイヤルホテル大阪
    • Related Report
      2011 Research-status Report
  • [Presentation] Quiescent gestational trophoblastic diseaseと考えられた2症例2011

    • Author(s)
      碓井宏和, 木原真紀, 加来博志, 錦見恭子, 山本憲子, 植原貴史, 加藤一喜, 楯真一, 三橋暁, 生水真紀夫.
    • Organizer
      第50回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      札幌コンベンションセンター
    • Related Report
      2011 Research-status Report
  • [Presentation] 症例に学ぶ 非順調型の臨床経過を辿る絨毛性疾患 低単位hCGの取扱いについて

    • Author(s)
      碓井宏和
    • Organizer
      第64回日本産科婦人科学会学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 婦人科悪性腫瘍と妊孕能温存 その2 絨毛性疾患

    • Author(s)
      碓井宏和
    • Organizer
      第53回日本婦人科腫瘍学会学術講演会
    • Place of Presentation
      岡山
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] he polymorphisms of folate metabolic enzyme gene and gestational trophoblastic disease

    • Author(s)
      Hirokazu Usui
    • Organizer
      IFPA Meeting 2012
    • Place of Presentation
      Hiroshima
    • Related Report
      2012 Research-status Report
  • [Presentation] Low-risk gestational trophoblastic neoplasiaに対するMTX療法の効果・副作用と葉酸代謝酵素遺伝子多型の関連 ~絨毛性腫瘍の個別化医療を目指して~

    • Author(s)
      碓井宏和、木原真紀、加来博志、山本憲子、錦見恭子、加藤一喜、楯真一、三橋暁、生水真紀夫
    • Organizer
      第64回日本産科婦人科学会学術講演会
    • Place of Presentation
      神戸
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.m.chiba-u.ac.jp/class/gyne/index.html

    • Related Report
      2013 Final Research Report
  • [Remarks] 千葉大学大学院医学研究院生殖医学

    • URL

      http://www.m.chiba-u.ac.jp/class/gyne/

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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