| Project/Area Number |
23592443
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ITO Naoki 岐阜大学, 医学部附属病院, 講師 (30184675)
FURUI Tatsurou 岐阜大学, 大学院医学系研究科, 准教授 (00313883)
TOYOKI Hiroshi 岐阜大学, 医学部附属病院, 講師 (40402169)
HAYASAKI You 岐阜大学, 大学院医学系研究科, 助教 (20566492)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | 卵巣癌 / 癌幹細胞 / 低酸素 / HIF / がん幹細胞 |
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| Research Abstract |
In the solid tumor, the region away from the vasculatures are hypoxic and recent studies have revealed that hypoxia inducible factor (HIF) increases the fraction of cancer stem cells (CSCs). TX-402, developed as a hypoxic cytotoxin, inhibited the expression of HIF.We supposed that TX-402 might be a possible candidate as a molecular-targeted drug for ovarian cancer by inhibiting CSC expansion. Hypoxia induced the expression of HIF, stem cell factors, and putative CSC markers. Furthermore, TX-402 inhibited these stem cell factors and CSC markers, dose-dependently.
Next, we constructed the spheroids of ovarian cancer cell line, as a 3D hypoxia model, in which the internal hypoxic region contained CSCs. These hypoxic regions containing CSCs was disappeared by the treatment with TX-402.In conclusion, hypoxia might promote the expansion of CSCs, and TX-402 could be useful for ovarian cancer by inhibiting the hypoxia-induced CSC expansion.
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