Quantitative analysis of nuclear chromatin distribution in uterine cervical squamous cell carcinoma, LSIL, HSIL and ASCUS.
| Project/Area Number |
23592454
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Kyushu University |
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Principal Investigator |
TSUNEHISA Kaku 九州大学, 医学(系)研究科(研究院), 教授 (60185717)
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| Co-Investigator(Kenkyū-buntansha) |
OHKI Masafumi 九州大学, 医学研究院, 教授 (10160441)
KOBAYASHI Hiroaki 九州大学, 医学研究院, 准教授 (70260700)
SUGISHIMA Setsuo 九州大学, 医学研究院, 教授 (50380382)
WATANABE Sumiko 九州大学, 医学研究院, 助教 (90404087)
田宮 貞史 九州大学, 医学(系)研究科(研究院), 准教授 (60284486)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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| Keywords | 細胞診断学 / 子宮頸癌 / 子宮頸部異形成 / 核クロマチン / ベゼスダ・システム / 扁平上皮癌 / LSIL / HSIL / ベセスダ・システム |
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| Research Abstract |
The chromatin pattern of nuclei is one of the most important criteria for cytological diagnosis. The quantitative assessment of nuclear chromatin distribution using image analysis by computer was applied for objective classification for cervical squamous epithelial lesions. An image analysis program was developed as a plug-in macro program of an image processing software, ImageJ. The radial distribution (RD) value that represents the gradient of the staining intensity from the center to the edge of a nucleus was defined as an index of the chromatin distribution. The RD values of LSIL (Low grade squamous intraepithelial lesion), HSIL (High grade squamous intraepithelial lesion) and squamous cell carcinoma were significantly difference among these three categories, and the RD value of HSIL was maximum. In the cervical squamous epithelial lesion, the RD value could be useful as an index for malignancy.
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Report
(4 results)
Research Products
(36 results)
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[Journal Article] Preoperative differential diagnosis of minimal deviation adenocarcinoma and lobular end cervical glandular hyperplasia of the uterine cervix : a multicenter study of clinico pathology and magnetic resonance imaging findings2011
Author(s)
Takatsu A, Shiozawa T, Miyamoto T, Kurosawa K, Kashima H, Yamada T, Kaku T, Mikami Y, Kiyokawa T, Tsuda H, Ishii K, Togashi K, Koyama T, Fujinaga Y, Kadoya M, Hashi A, Susumu N, Konishi I.
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Journal Title
Int J Gynecol Cancer
Volume: 21(7)
Issue: 7
Pages: 1287-96
DOI
Related Report
Peer Reviewed
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[Presentation] Correlation between nuclear chromatin pattern and cell cycle2013
Author(s)
Sumiko Watanabe, Tsunehisa Kaku, Masafumi Ohki, Sadafumi Tamiya, Setsuo Sugishima, Setsuko Murata, Yoshihiro Ohishi, Masatoshi Yokoyama, Yoshiko Kashimura, Masamichi Kashimura, Tsuyoshi Iwasaka
Organizer
18th INTERNATIONAL CONGRESS OF CYTOLOGY
Place of Presentation
PALAIS DES CONGRES(FRANCE PARIS)
Related Report
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[Presentation] Correlation between nuclear chromatin pattern and cell cycle.2013
Author(s)
Sumiko Watanabe, Tsunehisa Kaku, Masafumi Ohki, Sadafumi Tamiya, Setsuo Sugishima, Setsuko Murata, Yoshihiro Ohishi, Masatoshi Yokoyama, Yoshiko Kashimura, Masamichi Kashimura, Tsuyoshi Iwasaka
Organizer
18th INTERNATIONAL CONGRESS OF CYTOLOGY.
Place of Presentation
PALAIS DES CONGRES(FRANCE PARIS)
Related Report
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