• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Live imaging of the cochlear mitochondria and reactive oxygen spcies

Research Project

Project/Area Number 23592475
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionThe University of Tokyo

Principal Investigator

KIKKAWA Yayoi S.  東京大学, 医学部附属病院, 病院診療医 (00452350)

Co-Investigator(Kenkyū-buntansha) SAKAMOTO Takashi  東京大学, 医学部附属病院, 講師 (50323548)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords耳科学 / 抗加齢医学 / 内耳 / 有毛細胞 / 難聴
Outline of Final Research Achievements

In this study, we developed a real-time imaging method of reactive oxygen species (ROS) in the live inner ears (cochlea). Also, we analyzed the mechanisms of cochlear protective agents against ROS.
[Contents] (1) Live ROS imaging in the cochlea. (a) Mouse cochlear explants were imaged using an ultra-high-speed confocal microscope (Nikon A1R). Tomographic images were then 3D reconstructed to pinpoint the active position for producing inner ear ROS. (b) Live, in vivo ROS production was imaged using cochlear-exposed guinea pigs. (2) Monitoring mitochondria distribution in the damaged inner ear: Mouse cochlear explants were incubated in medium containing various concentrations of cisplatin, and mitochondria distribution and ROS production were examined. (3) Elucidation of anti-ROS mechanism in the inner ear: Mouse cochlear explants were cultured in cisplatin-containing medium with hydrogen gas dissolved directly into the media and ROS production and hair cell survival was evaluated.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • Research Products

    (4 results)

All 2015 2014

All Journal Article (4 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results)

  • [Journal Article] Prognostic factors for long-term hearing preservation after canal-tympanoplasty for congenital aural atresia.2015

    • Author(s)
      Sakamoto T, Kikuta S, Kikkawa YS, Kinoshita M, Saito Y, Kobayashi K, Kakigi A, Suzuki M, Yamasoba T
    • Journal Title

      Europian Archives of Otorhinolaryngology

      Volume: 272 Issue: 11 Pages: 3151-6

    • DOI

      10.1007/s00405-014-3164-6

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hydrogen protects auditory hair cells from cisplatin-induced free radicals2014

    • Author(s)
      Kikkawa Y. S., Nakagawa T., Taniguchi M., and Ito J
    • Journal Title

      Neurosci Lett

      Volume: 579 Pages: 125-129

    • DOI

      10.1016/j.neulet.2014.07.025

    • NAID

      120005466694

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Growth factor-eluting cochlear implant electrode: impact on residual auditory function, insertional trauma, and fibrosis2014

    • Author(s)
      Kikkawa Y. S., Nakagawa T., Ying L., Tabata Y., Tsubouchi H., Ido A., and Ito J.
    • Journal Title

      J Transl Med

      Volume: 12 Issue: 1 Pages: 280-280

    • DOI

      10.1186/s12967-014-0280-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Favorable prognostic factors for long-term postoperative hearing results after canal tympanoplasty for congenital aural stenosis2014

    • Author(s)
      Sakamoto T, Kikkawa YS, Kikuta S, Kinoshita M, Ueha R, Suzukawa K, Kashio A, Kakigi A, Ito K, Suzuki M, Yamasoba T
    • Journal Title

      Otology & Neurotology

      Volume: 35 Issue: 6 Pages: 966

    • DOI

      10.1097/mao.0000000000000335

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi