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Analysis of the kinocilia influence in the cochlea outer hair cells

Research Project

Project/Area Number 23592476
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionTokyo Medical and Dental University

Principal Investigator

TOKANO Hisashi  東京医科歯科大学, 医歯(薬)学総合研究科, 助教 (70334422)

Co-Investigator(Kenkyū-buntansha) KITAMURA Ken  東京医科歯科大学, 大学院医歯学総合研究科, 教授 (90010470)
KIKKAWA Yoshiaki  東京都立総合医学研究所, 哺乳類遺伝プロジェクト, プロジェクトリーダー (20280787)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsレプチン受容体 / 外有毛細胞 / 高血糖 / マウス / ストレプトゾトシン / アディポネクチン / C57BL/6J / レプチンレセプター / C3H/HeJ / 糖尿病 / 難聴 / PAS染色 / 酸化障害 / 8-OHdG
Research Abstract

Because C3H/HeJ mice have leptin receptor in their cochlear outer hair cell kinocilia, we analyze leptin receptor knock out Lepr db/db mice, which were known to model of diabetes mellitus. ABR thresholds were proximately same but accepted early attenuation in DPOAEs compared with control one, the responsible part of their hearing loss was regarded as outer hair cells. There were no stereocilia displacement and no hair cell bundle transformation but abnormal PAS stained follicles in cytoplasm, these meant DPOAEs attenuation were due to outer hair cell body disorder rather than stereocilia disfunction. On the other hand, in leptin receptor intact hyperglycosemia state C3H/HeJ mice, ABR thresholds and DPOAEs were proximately same compared with control one and abnormal PAS stained follicles in cytoplasm were never seen these mice. In conclusion, Absence of outer hair cell kinocilia were related to cytoplasmic abnormality and there ware no affection only in a simple hyperglycosemia state.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (26 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 3 results) Presentation (21 results)

  • [Journal Article] RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection?A technical report2013

    • Author(s)
      Kimura Y, Kubo S, Koda H, Shigemoto K, Sawabe M, Kitamura K
    • Journal Title

      Hear res

      Volume: 302 Pages: 26-31

    • DOI

      10.1016/j.heares.2013.04.008

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNA analysis of inner ear cells from formalin fixed paraffin embedded (FFPE) archival human temporal bone section using laser microdissection; A technical report.2013

    • Author(s)
      Kimura Y, Kubo S, Koda H, Shigemoto K, Sawabe M, Kitamura K
    • Journal Title

      Hearing Research

      Volume: 302 Pages: 26-31

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 糖尿病モデルマウスLeprdb/dbの聴覚機能及び形態学的解析2011

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村健
    • Journal Title

      日本耳科学会雑誌

      Volume: 21 Pages: 379-379

    • URL

      https://www.jstage.jst.go.jp/browse/otoljpn/21/0/_contents/-char/ja/

    • Related Report
      2013 Final Research Report
  • [Journal Article] Generating mouse models of degenerative diseases using Cre/lox-mediated in vivo mosaic cell ablation2011

    • Author(s)
      Fujioka M, Tokano H, Shiina-Fujioka K, Okano H and Edge AS
    • Journal Title

      J Clin Invest

      Volume: 121 Issue: 6 Pages: 2462-2469

    • DOI

      10.1172/jci45081

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 糖尿病モデルマウスLeprdb/dbの聴覚機能及び形態学的解析2011

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村 健
    • Journal Title

      日本耳科学会雑誌

      Volume: 21 Pages: 379-379

    • Related Report
      2011 Research-status Report
  • [Presentation] ペンドレッド症候群の新規マウスモデル2014

    • Author(s)
      伊藤卓,喜多村健
    • Organizer
      平成25年度第2回厚生労働省急性高度難聴調査研究班会議
    • Place of Presentation
      東京
    • Year and Date
      2014-02-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 培養前庭神経細胞における過分極誘発カチオンチャネルの日齢変化2014

    • Author(s)
      吉本亮一,喜多村健
    • Organizer
      平成25年度第2回厚生労働省急性高度難聴調査研究班会議
    • Place of Presentation
      東京
    • Year and Date
      2014-02-08
    • Related Report
      2013 Final Research Report
  • [Presentation] 培養前庭神経細胞における過分極誘発カチオンチャネルの日齢変化2014

    • Author(s)
      吉本亮一、喜多村 健
    • Organizer
      平成25年度第2回厚生労働省急性高度難聴調査研究班会議
    • Place of Presentation
      東京(慶応大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] ペンドレッド症候群の新規マウスモデル2014

    • Author(s)
      伊藤 卓、喜多村 健
    • Organizer
      平成25年度第2回厚生労働省急性高度難聴調査研究班会議
    • Place of Presentation
      東京(慶応大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 培養前庭神経節細胞における過分極誘発カチオンチャネルの特性2013

    • Author(s)
      吉本亮一,岩崎真一,鷹合秀輝,喜多村健
    • Organizer
      第23回日本耳科学会総会学術講演会
    • Place of Presentation
      宮崎
    • Year and Date
      2013-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 培養前庭神経節細胞における過分極誘発カチオンチャネルの特性2013

    • Author(s)
      吉本亮一、岩崎真一、高合秀輝、喜多村 健
    • Organizer
      第23回日本耳科学会総会学術講演会
    • Place of Presentation
      宮崎(シーガイアコンベンションセンター)
    • Related Report
      2013 Annual Research Report
  • [Presentation] Generating Mouse Models of Early Stages of Degenerative diseases Using Cre/lox-mediated in Vivo Mosaic Cell Abration2012

    • Author(s)
      Fujioka M, Tokano H, Mizutari K, Ogawa K, Okano H, Edge AS.
    • Organizer
      1st Asian Otology Meeting/ 3rd East Asian Symposium on Otology
    • Place of Presentation
      Nagasaki, Japan
    • Year and Date
      2012-06-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 突発性難聴純音聴力固定後の語音弁別能の変化2012

    • Author(s)
      野口佳裕、高橋正時、戸叶尚史、西尾綾子、山本 桂、喜多村 健
    • Organizer
      第113回日本耳鼻咽喉科学会総会
    • Place of Presentation
      新潟
    • Related Report
      2012 Research-status Report
  • [Presentation] Generating Mouse Models of Early Stages of Degenerative diseases Using Cre/lox-mediated in Vivo Mosaic Cell Abration.2012

    • Author(s)
      Fujioka M, Tokano H, Mizutari K, Ogawa K, Okano H, Edge AS.
    • Organizer
      The 1st Asian Otology Meeting/ 3rd East Asian Symposium on Otology
    • Place of Presentation
      Nagasaki
    • Related Report
      2012 Research-status Report
  • [Presentation] 高度難聴に対してステロイド鼓室内投与と高気圧酸素治療を併用した1例2012

    • Author(s)
      岸川正大、岩崎朱見、戸叶尚史、角田篤信、喜多村 健
    • Organizer
      第21回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 歪成分耳音響放射を検出し、かつ語音弁別能が不良であった症例の検討2012

    • Author(s)
      丸山絢子、戸叶尚史、吉本亮一、野口佳裕、喜多村 健
    • Organizer
      第21回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Presentation] 突発性難聴純音聴力固定後の語音弁別能、ABR、THIの変化2012

    • Author(s)
      野口佳裕、高橋正時、戸叶尚史、西尾綾子、山本 桂、澤田光毅、喜多村 健
    • Organizer
      第57回聴覚医学会総会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 東京医科歯科大学における原田病症例の聴平衡覚所見2012

    • Author(s)
      西尾綾子、野口佳裕、高橋正時、戸叶尚史、山本 桂、吉本亮一、澤田光毅、喜多村 健
    • Organizer
      第57回聴覚医学会総会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] 歪み成分耳音響放射が認められる語音弁別能不良例の検討2012

    • Author(s)
      丸山絢子、戸叶尚史、吉本亮一、野口佳裕、喜多村 健
    • Organizer
      第57回聴覚医学会総会
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Presentation] Cre/loxと調節性Caspaseを用いたモザイク様細胞死モデルの樹立?病気の「なりはじめを」科学する2011

    • Author(s)
      藤岡正人、戸叶尚史、小川郁
    • Organizer
      第21回日本耳科学会総会
    • Place of Presentation
      那覇
    • Year and Date
      2011-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病モデルマウスLeprdb/dbの聴覚機能及び形態学的解析2011

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村健
    • Organizer
      第21回日本耳科学会総会学術講演会
    • Place of Presentation
      那覇
    • Year and Date
      2011-11-24
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病モデルマウスの内耳におけるPAS染色陽性細胞の局在2011

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村健
    • Organizer
      第20回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病モデルマウス解析の現状2011

    • Author(s)
      戸叶尚史、岩崎朱見、鈴木康弘、喜多村健:
    • Organizer
      第20回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Year and Date
      2011-07-30
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病モデルマウス解析の現状

    • Author(s)
      戸叶尚史、岩崎朱見、鈴木康弘、喜多村 健
    • Organizer
      第20回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖尿病モデルマウスの内耳におけるPAS染色陽性細胞の局在

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村 健
    • Organizer
      第20回御茶ノ水耳鼻咽喉科頭頸部外科集談会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖尿病モデルマウスLeprdb/dbの聴覚機能及び形態学的解析

    • Author(s)
      岩崎朱見、戸叶尚史、鈴木康弘、喜多村 健
    • Organizer
      第21回日本耳科学会総会学術講演会
    • Place of Presentation
      那覇
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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