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new gene transduction to inner ear with confocal-micro endoscope

Research Project

Project/Area Number 23592480
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionKyoto University

Principal Investigator

TAURA Akiko  京都大学, 医学(系)研究科(研究院), 助教 (70515345)

Co-Investigator(Kenkyū-buntansha) NAKAGAWA Takayuki  京都大学, 大学院医学研究科, 講師 (50335270)
SAKAMOTO Tatsunori  京都大学, 大学院医学研究科, 助教 (60425626)
KITAJIRI Shinichiro  京都大学, 大学院医学研究科, 助教 (00532970)
EBISU Fumi  京都大学, 大学院医学研究科, 特定研究員 (70596197)
Co-Investigator(Renkei-kenkyūsha) FUNABIKI Kazuo   (00301234)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords内耳有毛細胞 / 遺伝子導入 / コンフォーカルマイクロエンドスコープ / 有毛細胞 / 内耳 / ポリエチレンイミン / カチオン化ポリマー / 穿孔パッチ法
Outline of Final Research Achievements

At present, it is difficult to transfer genes into hair cells in inner ear. Also it is hard to transfer genes into only hair cells. In this study, we identified hair cell and tried to induce GFP into hair cells. Using glass micro-pipette, we injected GFP gene into inner ear tissues and passed an electric current. By adding electric current, the transduction efficiency increased. Furthermore, we attached glass pipette to cell surface and make micropore chemically and passed weak electric current. In this method, we could successfully induce GFP genes into hair cells. However, the efficiency was not good.
Also using confocal micro endoscope, we could observe hair cells.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (23 results)

All 2015 2014 2013 2012 2011

All Journal Article (5 results) (of which Open Access: 3 results,  Peer Reviewed: 4 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Invited: 1 results) Book (5 results)

  • [Journal Article] トピックス 内耳再生医療2014

    • Author(s)
      田浦晶子
    • Journal Title

      Equilibrium Research

      Volume: 73 Pages: 538-542

    • Related Report
      2014 Annual Research Report
    • Open Access
  • [Journal Article] Effects of mouse utricle stromal tissues on hair cell induction from induced pluripotent stem cells.2014

    • Author(s)
      Taura A., Ohnishi H., Ochi S., Ebisu F., Nakagawa T., and Ito J
    • Journal Title

      BMC Neurosci

      Volume: 15 Issue: 1 Pages: 121-121

    • DOI

      10.1186/s12868-014-0121-7

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] One-third of vertiginous episodes during the follow-up period are caused by benign paroxysmal positional vertigo in patients with Meniere's disease2014

    • Author(s)
      Taura, A. Funabiki, K. Ohgita, H. Ogino, E. Torii, H. Matsunaga, M. Ito, J.
    • Journal Title

      Acta Otolaryngol

      Volume: 134 Issue: 11 Pages: 1140-5

    • DOI

      10.3109/00016489.2014.936624

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Benign paroxysmal positional vertigo related to allergic otitis.2011

    • Author(s)
      Akiko Taura ;Eriko Ogino ;Hideaki Ohgita ;Takayuki Nakagawa ;Kazuo Funabiki ;Juichi Ito.
    • Journal Title

      The American Journal of Case Reports.

      Volume: 12 Pages: 169-172

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Short-arm型後半規管BPPVが疑われた4症例の検討2011

    • Author(s)
      田浦晶子 ;三浦誠 ;扇田秀章 ;船曳和雄 ;伊藤壽一
    • Journal Title

      Equilibrium Research

      Volume: 70 Pages: 151-158

    • NAID

      10029512420

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] マウスiPS細胞から蝸牛有毛細胞誘導への試み2015

    • Author(s)
      田浦晶子
    • Organizer
      第14回 日本再生医療医学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞を用いた前庭障害治療2014

    • Author(s)
      田浦晶子
    • Organizer
      第73回 日本めまい平衡医学会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2014-11-05 – 2014-11-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] The Regenerative Therapy for Vestibular Disorders with human induced pluripotent stem (iPS) cells.2014

    • Author(s)
      Taura AKiko
    • Organizer
      Inner Ear Biology Workshop 2014
    • Place of Presentation
      Kyoto(JAPAN)
    • Year and Date
      2014-11-01 – 2014-11-04
    • Related Report
      2014 Annual Research Report
  • [Presentation] iPS細胞を用いた内耳再生治療への試み2014

    • Author(s)
      田浦晶子
    • Organizer
      第115回 日本耳鼻咽喉科学会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡県福岡市)
    • Year and Date
      2014-05-16
    • Related Report
      2014 Annual Research Report
  • [Presentation] ヒトiPS細胞を用いた前庭障害治療2014

    • Author(s)
      田浦晶子 、坂本達則、大西弘恵、中川隆之 、伊藤壽一
    • Organizer
      平成25年度前庭機能異常に関する調査研究報告会
    • Place of Presentation
      東京医大病院
    • Related Report
      2013 Research-status Report
  • [Presentation] ヒトiPS細胞を用いた前庭障害治療への試み2014

    • Author(s)
      田浦晶子、越智 翔平、大西弘恵、中川隆之、伊藤壽一
    • Organizer
      第67回日本再生医療医学会
    • Place of Presentation
      京都国際会議場
    • Related Report
      2013 Research-status Report
  • [Presentation] 未来の前庭障害治療2013

    • Author(s)
      田浦晶子
    • Organizer
      第4回大阪めまいフォーラム
    • Place of Presentation
      スイスホテル南海大阪
    • Related Report
      2013 Research-status Report
    • Invited
  • [Presentation] Ionophoresisによる遺伝子導入についての検討2012

    • Author(s)
      田浦晶子 ;中川隆之 ;伊藤壽一
    • Organizer
      第22回 日本耳科学会
    • Place of Presentation
      名古屋国際会議場(名古屋市)
    • Related Report
      2012 Research-status Report
  • [Presentation] PKC経路活性化による内耳障害治療2011

    • Author(s)
      田浦晶子 ;中川隆之 ;伊藤壽一
    • Organizer
      第21回 日本耳科学会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 内耳障害におけるPKC経路についての検討2011

    • Author(s)
      田浦晶子 ;中川隆之 ;伊藤壽一
    • Organizer
      第112 回 日本耳鼻咽喉科学会
    • Place of Presentation
      国立京都国際会館(京都市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 眼振ベクトル解析と眼球運動に関する検討2011

    • Author(s)
      田浦晶子 ;鳥居紘子 ;荻野枝里子 ;扇田秀章 ;船曳和雄 ;伊藤壽一
    • Organizer
      第70回 日本めまい平衡医学会
    • Place of Presentation
      ホテルニューオータニ幕張(千葉県)
    • Related Report
      2011 Research-status Report
  • [Presentation] 起立性低血圧症の眼球運動所見についての検討2011

    • Author(s)
      田浦晶子 ;鳥居紘子 ;荻野枝里子 ;扇田秀章 ;船曳和雄 ;伊藤壽一
    • Organizer
      第29回 頭頸部自律神経研究会
    • Place of Presentation
      ホテルグランヴィア大阪(大阪市)
    • Related Report
      2011 Research-status Report
  • [Presentation] 頸性めまい症例の特徴的検査所見の検討2011

    • Author(s)
      田浦晶子 ;鳥居紘子 ;荻野枝里子 ;扇田秀章 ;船曳和雄 ;伊藤壽一
    • Organizer
      第12回 京滋めまいカンファレンス
    • Place of Presentation
      メルパルク京都(京都府)
    • Related Report
      2011 Research-status Report
  • [Book] ガイドライン外来診療20142014

    • Author(s)
      田浦晶子
    • Total Pages
      6
    • Publisher
      日経メディカル開発
    • Related Report
      2014 Annual Research Report
  • [Book] Regenerative Medicine for the Inner Ear: Gene Therapy2014

    • Author(s)
      Taura Akiko
    • Total Pages
      7
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] Regenerative Medicine for the Inner Ear: Self repair2014

    • Author(s)
      Taura Akiko
    • Total Pages
      9
    • Publisher
      Springer
    • Related Report
      2014 Annual Research Report
  • [Book] ガイドライン外来診療20142014

    • Author(s)
      田浦晶子、中川隆之
    • Total Pages
      6
    • Publisher
      日経メディカル開発
    • Related Report
      2013 Research-status Report
  • [Book] Clinical Neuroscience2011

    • Author(s)
      田浦晶子 ;伊藤壽一
    • Publisher
      有毛細胞の再生治療
    • Related Report
      2011 Research-status Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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