• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Morphological regulation of the developmental inner ear hair cells by non-muscle myosin II

Research Project

Project/Area Number 23592491
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionKyoto Prefectural University of Medicine

Principal Investigator

SAKAGUCHI Hirofumi  京都府立医科大学, 医学(系)研究科(研究院), 准教授 (00515223)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords内耳 / ミオシン / 上皮細胞 / 有毛細胞 / 蝸牛 / 聴覚 / サルコメア / 頂側結合 / アクチン / II型ミオシン / 細胞間結合 / 国際情報交換 / アメリカ / 難聴 / 支持細胞
Research Abstract

Non-muscle myosin II (NMII) is known to regulate cell shapes through the contraction of actin filaments, major components of the cytoskeleton. In muscle cells, NMII is known to form a complex which alined alternately with actin filaments and to provide force by contracting this "sarcomere" structure. On the other hand, the mechanism for providing NMII-based contraction force in the epithelial cells is still unclear. In this research, we demonstrated that NMII localized to the apical cell junction in the inner ear sensory epithelia, forming sarcomeric structures with the apical actin ring to provide string-like contraction force to the apical surface of the cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2014 2013 2012 2011 Other

All Journal Article (4 results) (of which Peer Reviewed: 3 results) Presentation (13 results) (of which Invited: 1 results) Remarks (2 results)

  • [Journal Article] NMII forms a contractile transcellular sarcomeric network to regulate apical cell junctions and tissue geometry2013

    • Author(s)
      Ebrahim S, Fujita T, Millis BA, Kozin E, Ma X, Kawamoto S, Baird MA, Davidson M, Yonemura S, Hisa Y, Conti MA, Adelstein RS, Sakaguchi H, Kachar B
    • Journal Title

      Curr Biol

      Volume: 23 Pages: 731-6

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 発生期蝸牛有毛細胞の形態変化とmyosin IIの関与2013

    • Author(s)
      藤田朋己,坂口博史,米村重信,久育男
    • Journal Title

      耳鼻咽喉科ニューロサイエンス

      Volume: 27 Pages: 19-21

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] NMII Forms a Contractile Transcellular Sarcomeric Network to Regulate Apical Cell Junctions and Tissue Geometry.2013

    • Author(s)
      Ebrahim S, Fujita T, Millis BA, Kozin E, Ma X, Kawamoto S, Baird MA, Davidson M, Yonemura S, Hisa Y, Conti MA, Adelstein RS, Sakaguchi H, Kachar B
    • Journal Title

      Current Biology

      Volume: 23(8) Issue: 8 Pages: 731-6

    • DOI

      10.1016/j.cub.2013.03.039

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 発生期蝸牛有毛細胞の形態変化とmyosin IIの関与2013

    • Author(s)
      藤田朋己,坂口博史,米村重信,久 育男
    • Journal Title

      耳鼻 咽喉科ニューロサイエンス

      Volume: 27 Pages: 19-21

    • Related Report
      2013 Annual Research Report
  • [Presentation] The morphological change of Reticular Lamina by three myosin II inhibitors2014

    • Author(s)
      Fujita T, Sakaguchi H, Yonemura S, Hisa Y
    • Organizer
      36th Association for Research in Otolaryngology (ARO) Midwinter Meeting
    • Place of Presentation
      サンディエゴ
    • Related Report
      2013 Final Research Report
  • [Presentation] The M orphological C hange of Reticular Lamina by T hree M yosin II I nhibitors2014

    • Author(s)
      Tomoki Fujita; Hirofumi Sakaguchi; Sigenobu Yonemura; Yasuo Hisa
    • Organizer
      The 37th MidWinter Meeting of the Association for Research in Otolaryngology
    • Place of Presentation
      San Diego, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] Molecular Mechanism of the Dynamic Morphological Regulation in Hair Cells2013

    • Author(s)
      Sakaguchi H
    • Organizer
      The 20th IFOS Congress
    • Place of Presentation
      ソウル(シンポジウム)
    • Related Report
      2013 Final Research Report
  • [Presentation] The morphological change of Reticular Lamina by ROCK inhibitor"2013

    • Author(s)
      Fujita T, Sakaguchi H, Yonemura Y, Hisa Y
    • Organizer
      36th Association for Research in Otolaryngology (ARO) Midwinter Meeting
    • Place of Presentation
      ボルチモア
    • Related Report
      2013 Final Research Report
  • [Presentation] The involvement of ROCK pathway in the apical cell contraction in murine organ of Corti2013

    • Author(s)
      Tomoki Fujita, Hirofumi Sakaguchi, Sigenobu Yonemura, Yasuo Hisa
    • Organizer
      36th Association for Research in Otolaryngology MidWinter Meeting
    • Place of Presentation
      Baltimore, USA
    • Related Report
      2012 Research-status Report
  • [Presentation] II型ミオシンによる有毛細胞形態の制御機構2012

    • Author(s)
      坂口博史,藤田朋己,足立直子,神谷透,中村高志,瀧正勝,兵庫美砂子,鈴木敏弘,米村重信,久育男.
    • Organizer
      第113回日本耳鼻咽喉科学会
    • Place of Presentation
      新潟
    • Related Report
      2013 Final Research Report
  • [Presentation] From Bench to Clinic : Next Generation in Otology. Dynamic Regulation of the Apical Hair Cell Structures2012

    • Author(s)
      Sakaguchi H
    • Organizer
      The 14th Japan-Korea Joint Meeting of ORL-HS
    • Place of Presentation
      京都(シンポジウム)
    • Related Report
      2013 Final Research Report
  • [Presentation] 内耳発生・再生に関する基礎研究.内耳感覚上皮細胞の形態形成?ミオシンによる動的制御に関して?2012

    • Author(s)
      坂口博史
    • Organizer
      第22回日本耳科学会.
    • Place of Presentation
      名古屋(シンポジウム)
    • Related Report
      2013 Final Research Report
  • [Presentation] Dynamic Structural Regulation in Hair Cells.2012

    • Author(s)
      Sakaguchi H.
    • Organizer
      Collegium Oto-Rhino-Laryngologicum Amicitiae Sacrum
    • Place of Presentation
      Rome, Italy
    • Related Report
      2012 Research-status Report
  • [Presentation] Dynamic Regulation of the Apical Hair Cell Structures.2012

    • Author(s)
      Sakaguchi H.
    • Organizer
      14th Japan-Korea Joint Meeting of Otorhinolaryngology
    • Place of Presentation
      Kyoto, Japan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 坂口博史2012

    • Author(s)
      内耳感覚上皮細胞の形態形成  ―ミオシンによる動的制御に関して―
    • Organizer
      第22回 日本耳科学会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
  • [Presentation] 坂口博史、藤田朋己、神谷 透、中村高志、足立直子、 瀧 正勝、鈴木敏弘、米村重信、久 育男2012

    • Author(s)
      II型ミオシンによる 有毛細胞形態の制御機構
    • Organizer
      第113回 日本耳鼻咽喉科学会学術講演会
    • Place of Presentation
      新潟
    • Related Report
      2012 Research-status Report
  • [Presentation] The Role of Actomyosin Contractility in Shaping the Apical Circumference of Hair Cells2011

    • Author(s)
      Fujita T, Hirofumi S, Suzuki T, Yonemura S, Hisa Y
    • Organizer
      48th Inner Ear Biology Workshop
    • Place of Presentation
      Lisbon
    • Related Report
      2011 Research-status Report
  • [Remarks] 京都府立医科大学耳鼻咽喉科/研究/内耳研 究グループ

    • URL

      http://www.f.kpu-m.ac.jp/k/ent/research

    • Related Report
      2013 Final Research Report
  • [Remarks] 京都府立医科大学耳鼻咽喉科・頭頸部外科学教室>研究>内耳研究グループ

    • URL

      http://www.f.kpu-m.ac.jp/k/ent/research.html#2

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi