| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
To investigate the autophagy in inner ear cultured cell line of HEI-OC1 under the oxidative stress condition, GFP-LC3 was introduced to HEI-OC1. As a result, the appearance of GFP-LC3 was observed under the confocal microscope in cytoplasm of the cultured cell. In addition the necrosis was confirmed morphologically. From the observation of autophagic vacuoles was confirmed under the electron microscope, the both autophagy and necrosis was observed in the inner ear culture cell under the oxidative stress condition. Rapamysine as a autophagy inducer was applied the HEI-OC1 treated with reactive oxygen, the cell viability was significantly improved than Rapamysine (-) condition.
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