analysis on the number of osteoclasts in auditory ossicles in middle ea disease and middle ear development

• Project/Area Number: 23592495
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Keio University
• Principal Investigator: KANZAKI Sho 慶應義塾大学, 医学部, 講師 (50286556)
• Co-Investigator(Renkei-kenkyūsha): 松尾 光一 慶應義塾大学, 医学部, 教授 (40229422)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 耳小骨 / 破骨細胞 / 中耳炎 / 真珠腫 / 中耳 / 難聴 / 耳硬化症

Outline of Final Research Achievements

1) Bone remodeling may be important for auditory ossicular structure. Osteoclasts are specialized multinuclear macrophages that secrete HCL and hydrolases causing bone resorption. Therefore, regulating of the number of osteoclastsis critical for maintaining the shape and size of auditory ossicles. We used Cg-Gt(ROSA)26sor<tm14(CAG-tdTomato)Hez/J4 mice to analyze the location of osteoclasts during development. The signals were shown in mice aged postnatal day 4.
2) osteoclasts in patients with cheloesteatoma We counted the number of Cathepsin K positive cells which are markers of osteoclasts. There are significant differences of the number of cathepsin K cells between normal people and patients with cholesteatoma.
The number of Cathepsin K positive cells and/or osteoclasts are significantly increased in the ossicles in cases of cholesteatoma. The anti-bone-resorptive medication may prevent bone resorption of cholesteatoma.

Research Products

• [Journal Article] Panel 3: Recent advances in anatomy, pathology, and cell biology in relation to otitis media pathogenesis. (2013)
  • Author(s): Cayé-Thomasen P, Hermansson A, Bakaletz L, Hellstrøm S, Kanzaki S, Kerschner J, Lim D, Lin J, Mason K, Spratley J.
  • Journal Title: Otolaryngol Head Neck Surg Volume: 148
  • Peer Reviewed
• [Journal Article] Panel 3: recent advances in anatomy, pathology, and cell biology in relation to otitis media pathogenesis. (2013)
  • Author(s): Cayé-Thomasen P, Hermansson A, Bakaletz L, Hellstrøm S, Kanzaki S, Kerschner J, Lim D, Lin J, Mason K, Spratley J.
  • Journal Title: Otolaryngol Head Neck Surg. Volume: 148(4 Suppl): Issue: S4
  • DOI: 10.1177/0194599813476257
• [Journal Article] Osteoclasts Regulate for Maintaining Morphology of Auditory Ossicles, (2013)
  • Author(s): Kanzaki S, Takada Y, Ogawa K, Matsuo K,
  • Journal Title: Middle Ear and Hearing 9thInternational Cholesteatoma and Ear Surgery Meeting Volume: proceeding
• [Journal Article] Regulating Osteoclasts for the Maintenance of Auditory Ossicular Morphology, the Middle Ear and Hearing (2012)
  • Author(s): Kanzaki S et al
  • Journal Title: Recent Advances in Otitis Media Volume: in press
• [Presentation] Regulation of osteoclasts is required to maintain morphology and function of ossicles in middle ear” (2012)
  • Author(s): Kanzaki S
  • Organizer: (Symposium) Bone resorption, Cholesteatoma and Ear surgery Meeting
  • Place of Presentation: Nagasaki Japan
  • Invited
• [Presentation] Regulating Osteoclasts for the Maintenance of Auditory Ossicular Morphology, the Middle Ear and Hearing (2011)
  • Author(s): Kanzaki S et al
  • Organizer: 10th International Symposium on Recent Advances in Otitis Media(招待講演)
  • Place of Presentation: New Orleans, USA