| Project/Area Number |
23592496
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Juntendo University |
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Principal Investigator |
MURATA junko 順天堂大学, 医学部, 准教授 (80332740)
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| Co-Investigator(Kenkyū-buntansha) |
KIMURA Thoru 北里大学, 理学部, 教授 (50280962)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | Pi3K/Aktシグナル伝達系 / PTEN / PTEN ckoマウス / ほ乳類 / 内耳発生 / 感覚上皮予定領域 / 有毛細胞 / PI3K/Aktシグナル径路 / 蝸牛 / PI3K/Aktシグナル経路 / PTEN cko マウス / convergent extension / PI3/Aktシグナル伝達系 |
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| Research Abstract |
PTEN, a product of a tumor suppressor gene, inhibits the PI3K/Akt pathway by dephosphorylating phosphatidylinositol (3,4,5)-triphosphate. We tried to elucidate the functions of PTEN during mammalian cochlear development by deleting PTEN conditionally in the mouse inner ear.
In PTEN-conditional knockout (cko) mutant cochlea, the diameter of cochlear ducts was increased and the cell proliferation continued till the later stage outside of the prosensory region. Extra rows of OHCs and IHCs were observed, whereas the average length of the cochlear ducts was shorter than that of the control mice. The expression patterns of early prosensory markers were almost same between the PTEN-CKO and the control mice. However, the hair cell differentiation was accelerated in the PTEN-CKO cochleae. The results indicate that PTEN may regulate the cell differentiation and proliferation after the prosensory determination, and contribute to the convergent extension of the cochlear duct.
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