| Project/Area Number |
23592500
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAKAMOTO Takashi 東京大学, 医学部附属病院, 講師 (50323548)
KASHIO Akinori 東京大学, 医学部附属病院, 助教 (20451809)
IKEMIYAGI Yoshihiro 東邦大学, 医学部, 助教 (50439931)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMASOBA Tatsuy 東京大学, 医学部附属病院, 教授 (60251302)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | C57BL/6 マウス / 加齢性難聴 / polyethyleneimine / 陰性荷電 / IgG / 免疫組織学 / 血管条 / 毛細血管基底膜 / 血液―内耳関門 / 毛細血管 / 基底膜 / ヘパラン硫酸プロテオグリカン / 基底膜陰性荷電 / C57BL/6マウス / 蝸牛 / ヘパラン硫酸 |
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| Research Abstract |
Purpose: To examine age-related morphological changes in the cochlear lateral wall. Methods: C57BL/6 mice were grouped according to age as follows: 3 days, 4 weeks, 8 weeks, 6months and 12 months. Polyethyleneimine (PEI) was used to examine changes in the basement membrane anionic sites(BMAS) of the strial vessels. Serial mid-modiolarparaffin thin sections were performed to measure the widths of the stria vascularis, and then incubate with anti-mouse IgG antibody. Results: In a width of the stria vascularis, a significant difference was observed between the 4- week- and 12- month-old mice. A statistically significant difference was observed in the distribution of PEI particles between 3-day- and 8-week-, 6- and 12- month-old mice. IgG deposition was detected in the strial capillary BM in 8-week-old, 6- month-old, and 12-month-old mice. Since IgG is a cationized antibody molecule, the decrease in the BMAS shown may be due to IgG deposition in the strial vessels.
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