| Project/Area Number |
23592502
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kawasaki Medical School |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Hisaki 川崎医科大学, 医学部, 講師 (70309574)
MORITA Norimasa 川崎医科大学, 医学部, 講師 (40341119)
TACHI Toshihiro 川崎医科大学, 医学部, 助教 (70341120)
浜本 真一 川崎医科大学, 医学部, 助教 (30551204)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 耳科学 / スパーオキサイド / 内耳 / 一酸化窒素（NO) / 薬剤性内耳障害 / スーパーオキサイド / CDDP / 一酸化窒素（ＮＯ） / ＣＤＤＰ |
|---|
| Research Abstract |
Free radicals, particularly nitrogen monoxide (NO), are involved in the pathology of various conditions. Several studies have reported that NO is also intimately involved in the pathology of sudden-onset and drug-induced hearing loss, but the results appear speculative. For this reason, we examined the role of NO and nitrogen monoxide synthetase (NOS) in the inner ear, using previously reported guinea pig with inner ear disorders induced by cisplatin administration. The following results were obtained:1)The obvious presence of NO in the lymphatic fluid of the tympanic cavity was detected, but measured values were not consistent;2)real-time polymerase chain reaction and western blotting revealed levels of NOS in inner ear samples; and 3)the level of NOS expression correlated with the auditory brain response threshold. Based on these findings, NOS is likely involved in inner ear disorders.
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