Identification of tumor suppressive nucleic acid medicine in maxillary sinus squamous cell carcionma
| Project/Area Number |
23592505
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Chiba University |
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Principal Investigator |
HANAZAWA Toyoyuki 千葉大学, 医学(系)研究科(研究院), 准教授 (90272327)
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| Co-Investigator(Kenkyū-buntansha) |
SEKI Naohiko 千葉大学, 大学院医学研究院, 准教授 (50345013)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | microRNA / 上顎洞癌 / 癌抑制遺伝子 / miR-874 / HDAC1 / 上顎癌 / マイクロRNA / がん / ゲノム |
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| Research Abstract |
Our recent studies of microRNA (miRNA) expression signature of maxillary sinus squamous cell carcinoma (MSSCC) demonstrated that microRNA-874 (miR-874) was significantly downregulated in cancer tissues, suggesting miR-874 function as a tumor suppressor. Restoration of mature miR-874 significantly inhibited cancer cell proliferation and induced apoptosis. Expression data of miR-874 transfectants and in silico analysis demonstrated that histone deacetylase 1 (HDAC1) was a direct target of miR-874 regulation. Silencing of the HDAC1 gene significantly inhibited cell proliferation and induced G2/M arrest and cell apoptosis in cancer cell. Downregulation of miR-874 was a frequent event in HNSCC. miR-874 acted as a tumor suppressor and directly targeted HDAC1. Recognition of tumor-suppressive miRNA-mediated cancer pathways provides new insights into the potential mechanisms of MSSCC oncogenesis and suggests novel therapeutic strategies for the disease.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] Tumour-suppressive microRNA-29s inhibit cancer cell migration and invasion by targeting laminin-integrin signalling in head and neck squamous cell carcinoma.2013
Author(s)
Kinoshita T, Nohata N, Hanazawa T, Kikkawa N, Yamamoto N, Yoshino H, Itesako T, Enokida H, Nakagawa M, Okamoto Y, Seki N.
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Journal Title
Br J Cancer
Volume: 109
Issue: 10
Pages: 2636-2645
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma.2013
Author(s)
Nohata N, Hanazawa T, Kinoshita T, Inamine A, Kikkawa N, Itesako T, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N.
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Journal Title
Br J Cancer
Volume: 108
Issue: 8
Pages: 1648-1658
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumor suppressive microRNA-375 regulates lactate dehydrogenase B in maxillary sinus squamous cell carcinoma2012
Author(s)
Kinoshita T, Nohata N, Yoshino H, Hanazawa T, Kikkawa N, Fujimura L, Chiyomaru T, Kawakami K, Enokida H, Nakagawa M, Okamoto Y, Seki N.
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Journal Title
Int J Oncol
Volume: 40
Pages: 185-93
DOI
Related Report
Peer Reviewed
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[Journal Article] Actin-related protein 2/3 complex subunit 5 (ARPC5) contributes to cell migration and invasion and is directly regulated by tumor-suppressive microRNA-133a in head and neck squamous cell carcinoma2012
Author(s)
Kinoshita T, Nohata N, Watanabe-Takano H, Yoshino H, Hidaka H, Fujimura L, Fuse M, Yamasaki T, Enokida H, Nakagawa M, Hanazawa T, Okamoto Y, Seki N.
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Journal Title
Int. J. Oncol
Volume: (印刷中)
Pages: 1770-8
DOI
Related Report
Peer Reviewed
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[Journal Article] Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion through targeting laminin-332 in head and neck squamous cell carcinoma.2012
Author(s)
Kinoshita T, Hanazawa T, Nohata N, Kikkawa N, Enokida H, Yoshino H, Yamasaki T, Hidaka H, Nakagawa M, Okamoto Y, Seki N.
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Journal Title
Oncotarget
Volume: 3
Pages: 1386-400
Related Report
Peer Reviewed
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[Presentation] Tumor suppressive microRNA-218 inhibits cancer cell migration and invasion through targeting laminin-332 in head and neck squamous cell carcinoma2013
Author(s)
Takashi Kinoshita, Toyoyuki Hanazawa, Nijiro Nohata, Naoko Kikkawa, Noriko Yamamoto, Hideki Enokida, Hirofumi Yoshino, Takeshi Yamasaki, Hideo Hidaka, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
Organizer
AACR Annual 104th Meeting
Place of Presentation
Washington, DC, USA)
Related Report
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[Presentation] Tumor suppressive microRNA-1 and microRNA-133a cluster regulating molecular targets in head and neck squamous cell carcinoma2012
Author(s)
Nijiro Nohata, Toyoyuki Hanazawa, Takashi Kinoshita, Naoko Kikkawa, Daiju Sakurai, Miki Fuse, Hirofumi Yoshino, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
Organizer
8th International Conference on Head and Neck Cancer
Place of Presentation
Toronto, ON, Canada
Related Report
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[Presentation] microRNA-874 as a tumor suppressor in maxillary sinus squamous cell carcinoma based on microRNA expression signature2012
Author(s)
Toyoyuki Hanazawa, Nijiro Nohata, Takashi Kinoshita,Naoko Kikkawa,Miki Fuse, Takeshi Chiyomaru, Hirofumi Yoshinoi, Hideki Enokida, Masayuki Nakagawa, Yoshitaka Okamoto, Naohiko Seki
Organizer
AACR 103rd Annual Meeting
Place of Presentation
Chicago, IL, USA
Related Report
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