Mucosal immunity in the upper respiratory tract

• Project/Area Number: 23592512
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Oita University
• Principal Investigator: SUZUKI MASASHI 大分大学, 医学部, 教授 (60211314)
• Co-Investigator(Kenkyū-buntansha): HIRANO Takashi 大分大学, 医学部, 講師 (20305056) ; 野田 謙二 大分大学, 医学部, 助教 (90511171)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 粘膜免疫 / 樹状細胞 / NKT細胞 / 上気道 / Th17細胞

Research Abstract

The efficacy of alpha-galactosylceramide (aGalCer) as a mucosal adjuvant was examined. Mice were immunized intranasally with nontypeable Haemophilus influenzae (NTHi) P6 protein and a-GalCer. P6-specific antibody responses in the form of P6-specific IgA in nasal washes and serum IgG titers were significantly elevated. Splenic CD4+ T cells expressed P6-specific Th1 and Th2 cytokine mRNA. In addition, NTHi was quantified in nasal washes following NTHi challenges, and the clearance of NTHi from the nasopharynx was also enhanced. These results indicate that aGalCer might be an effective mucosal adjuvant.

Research Products

• [Journal Article] Pli play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarhalis (2013)
  • Author(s): Kawano T, Hirano T, Kodama S, Mitsui MT, Ahmed K, Nishizono A, Suzuki M
  • Journal Title: Pathog Dis Volume: 67 Pages: 119-131
  • Peer Reviewed
• [Journal Article] Effect of low-intensity focused ultrasound on the middle ear in a mouse model of acute otitis media (2013)
  • Author(s): Noda K, Hirano T, Noda K, Kodama S, Ichimiya I, Suzuki M.
  • Journal Title: Ultrasound Med Biol Volume: 39 Pages: 413-423
  • Peer Reviewed
• [Journal Article] Pli play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarhalis. (2013)
  • Author(s): Kawano T, Hirano T, Kodama S, Mitsui MT, Ahmed K, Nishizono A, Suzuki M.
  • Journal Title: Pathog Dis Volume: 67 Issue: 3 Pages: 119-131
  • DOI: 10.1016/j.ultrasmedbio.2012.10.003
  • Peer Reviewed
• [Journal Article] Pili play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarrhalis. (2013)
  • Author(s): Kawano T, Hirano T, Kodama S, Mitsui MT, Ahmed K, Nishizono A, Suzuki M.
  • Journal Title: Pathogens Dis Volume: 67 Pages: 119-131
  • Peer Reviewed
• [Journal Article] Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenza-specific long term mucosal immune responses in the nasopharynx (2011)
  • Author(s): Kodama S, Hirano T, Noda K, Umemoto S, Suzuki M
  • Journal Title: Vaccine Volume: 29 Pages: 1881-1890
  • Peer Reviewed
• [Journal Article] A single nasal dose of CCL20 chemokine induces dendritic cell recruitment and enhances nontypeable Haemophilus influenzae-specific immune responses in the nasal mucosa (2011)
  • Author(s): Kodama S, Abe N, Hirano T, Suzuki M
  • Journal Title: Acta Otolaryngol Volume: 131 Pages: 989-996
  • Peer Reviewed
• [Journal Article] Nasal-associated lymphoid tissue immunity and vaccine development (2011)
  • Author(s): Kodama S, Suzuki M
  • Journal Title: Adv Otorhinolaryngol Volume: 172 Pages: 110-112
  • Peer Reviewed
• [Journal Article] Th17 cells contribute to nontypeable Haemophilus influenza-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and a-galactosylceramide (2011)
  • Author(s): Noda K, Kodama S, Umemoto S, Nomi N, Hirano T, Suzuki M
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 584-581
  • Peer Reviewed
• [Journal Article] Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenza and Moraxella catarrhalis fron the nasopharynx in mice (2011)
  • Author(s): Hirano T, Kodama S, Kawano T, Maeda K, Suzuki M
  • Journal Title: FEMS immunol Med Microbiol Volume: 63 Pages: 407-417
  • Peer Reviewed
• [Journal Article] Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and a-galactosylceramide (2011)
  • Author(s): Noda K, Kodama S, Umemoto S, Nomi N, Hirano T, Suzuki M
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 584-581
  • Peer Reviewed
• [Journal Article] Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenzae and Moraxella catarrhalis fron the nasopharynx in mice (2011)
  • Author(s): Hirano T, Kodama S, Kawano T, Maeda K, Suzuki M
  • Journal Title: FEMS immunol Med Microbiol Volume: 63 Pages: 407-417
  • Peer Reviewed
• [Journal Article] Expression of bone morphogenic protein in sinonasal inverted papilloma with new bone formation (2011)
  • Author(s): Okamoto T, Kodama S, Nomi N, Umemoto S, Suzuki M
  • Journal Title: Allergy Rhinol Volume: 2 Pages: 16-20
  • Peer Reviewed
• [Journal Article] Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and α-galactosylceramide. (2011)
  • Author(s): Noda K, Kodama S, Umemoto S, Nomi N, Hirano T, Suzuki M.
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 574-581
  • NAID: 10031121506
  • Peer Reviewed
• [Presentation] Vaccination in otitis media (2013)
  • Author(s): Kodama S, Suzuki M
  • Organizer: 20th IFOS(第20回世界耳鼻咽喉科学会)
  • Place of Presentation: Seoul
  • Year and Date: 2013-06-01
• [Presentation] 上気道粘膜免疫におけるNKT細胞の機能解析 (2013)
  • Author(s): 梅本真吾、児玉悟、野田謙二、鈴木正志
  • Organizer: 第31回日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 倉敷市
• [Presentation] NKT細胞による上気道粘膜免疫応答の解析 (2012)
  • Author(s): 梅本真吾、児玉悟、野田謙二、鈴木正志
  • Organizer: 日本耳鼻咽喉科免疫アレルギー学会
  • Place of Presentation: 大津市
• [Presentation] Vaccination in otitis media.
  • Author(s): Kodama S, Suzuki M．
  • Organizer: 20th IFOS
  • Place of Presentation: COEX Convention Center, Seoul, Korea
  • Invited
• [Remarks]
  • URL: http://www.med.oita-u.ac.jp/ent/