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Dendritic cell-targeted nasal vaccine development

Research Project

Project/Area Number 23592513
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Otorhinolaryngology
Research InstitutionOita University

Principal Investigator

KODAMA SATORU  大分大学, 医学部, 講師 (40325717)

Co-Investigator(Kenkyū-buntansha) SUZUKI Masashi  大分大学, 医学部, 教授 (60211314)
NOMI Nozomi  大分大学, 医学部, 助教 (40468020)
野田 謙二  大分大学, 医学部, 助教 (90511171)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords樹状細胞 / 上気道 / 粘膜免疫 / 経鼻ワクチン / NKT細胞 / DNAワクチン / 上気道感染症
Research Abstract

Dendritic cells (DCs) are essential for the induction of antigen (Ag)-specific immune responses, DC-targeted vaccination might be an effective strategy for the induction of the specific immune responses. A DNA plasmid encoding the P6 outer membrane protein of nontypeable Haemophilus influenzae (NTHi) was constructed. Mice were immunized 3 times intranasally with the DNA plasmid and Matrix-M, an immunostimulatory complex adjuvant. P6-specific nasal wash IgA and serum IgG were elevated following immunization with the DNA plasmid and Matrix-M. The number of specific IgA-producing cells increased in the nasal passages of the immunized mice. Moreover, DNA vaccination enhanced bacterial clearance. These findings suggest that a successful DNA vaccination protocol has been developed. Nasal vaccination with P6 DNA vaccine and Matrix-M might be a new effective regimen for the induction of specific protective immunity in the upper respiratory tract.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (20 results)

All 2013 2012 2011 Other

All Journal Article (14 results) (of which Peer Reviewed: 13 results) Presentation (5 results) (of which Invited: 1 results) Remarks (1 results)

  • [Journal Article] Pli play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarhalis2013

    • Author(s)
      Kawano T, Hirano T, Kodama S, Mitsui MT, Ahmed K, Nishizono A, Suzuki M
    • Journal Title

      Pathog Dis

      Volume: 67 Pages: 119-131

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effect of low-intensity focused ultrasound on the middle ear in a mouse model of acute otitis media2013

    • Author(s)
      Noda K, Hirano T, Noda K, Kodama S, Ichimiya I, Suzuki M
    • Journal Title

      Ultrasound Med Biol

      Volume: 39 Pages: 413-423

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pili play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarrhalis2013

    • Author(s)
      Kawano T.,Hirano T.,Kodama S., Mitsui MT.,Ahmed K.,Nishizono A., Suzuki M
    • Journal Title

      pathogens and disease

      Volume: 67 Issue: 2 Pages: 119-131

    • DOI

      10.1111/2049-632x.12025

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pli play an important role in enhancing the bacterial clearance from the middle ear in a mouse model of acute otitis media with Moraxella catarhalis.2013

    • Author(s)
      Kawano T, Hirano T, Kodama S, Mitsui MT, Ahmed K, Nishizono A, Suzuki M.
    • Journal Title

      Pathog Dis

      Volume: 67 Issue: 3 Pages: 119-131

    • DOI

      10.1016/j.ultrasmedbio.2012.10.003

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] 経鼻ワクチンによる免疫誘導2012

    • Author(s)
      児玉悟
    • Journal Title

      日本鼻科学会誌

      Volume: 51 Pages: 58-59

    • NAID

      130004546123

    • Related Report
      2013 Final Research Report
  • [Journal Article] Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenza-specific long term mucosal immune responses in the nasopharynx2011

    • Author(s)
      Kodama S, Hirano T, Noda K, Umemoto S, Suzuki M
    • Journal Title

      Vaccine

      Volume: 29 Pages: 1881-1890

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A single nasal dose of CCL20 chemokine induces dendritic cell recruitment and enhances nontypeable Haemophilus influenzae-specific immune responses in the nasal mucosa2011

    • Author(s)
      Kodama S, Abe N, Hirano T, Suzuki M
    • Journal Title

      Acta Otolaryngol

      Volume: 131 Pages: 989-996

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nasal-associated lymphoid tissue immunity and vaccine development2011

    • Author(s)
      Kodama S, Suzuki M
    • Journal Title

      Adv Otorhinolaryngol

      Volume: 72 Pages: 110-112

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Th17 cells contribute to nontypeable Haemophilus influenza-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and a-galactosylceramide2011

    • Author(s)
      Noda K, Kodama S, Umemoto S, Nomi N, Hirano T, Suzuki M
    • Journal Title

      Microbiol Immunol

      Volume: 55 Pages: 584-581

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenza and Moraxella catarrhalis fron the nasopharynx in mice2011

    • Author(s)
      Hirano T, Kodama S, Kawano T, Maeda K, Suzuki M
    • Journal Title

      FEMS immunol Med Microbiol

      Volume: 63 Pages: 407-417

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Th17 cells contribute to nontypeable Haemophilus influenzae-specific protective immunity induced by nasal vaccination with P6 outer membrane protein and a-galactosylceramide2011

    • Author(s)
      Noda K, Kodama S, Umemoto S, Nomi N, Hirano T, Suzuki M
    • Journal Title

      Microbiol Immunol

      Volume: 55 Pages: 584-581

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Hirano T, Kodama S, Kawano T, Maeda K, Suzuki M. Monophosphoryl lipid A induced innate immune responses via TLR4 to enhance clearance of nontypeable Haemophilus influenzae and Moraxella catarrhalis fron the nasopharynx in mice.
    • Journal Title

      FEMS immunol Med Microbiol

      Volume: 63 Pages: 407-417

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Expression of bone morphogenic protein in sinonasal inverted papilloma with new bone formation2011

    • Author(s)
      Okamoto T, Kodama S, Nomi N, Umemoto S, Suzuki M
    • Journal Title

      Allergy Rhinol

      Volume: 2 Pages: 16-20

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Nasal immunization with plasmid DNA encoding P6 protein and immunostimulatory complexes elicits nontypeable Haemophilus influenzae-specific long-term mucosal immune responses in the nasopharynx.2011

    • Author(s)
      Kodama S, Hirano T, Noda K, Umemoto S, Suzuki M.
    • Journal Title

      Vaccine

      Volume: 29 Pages: 1881-1890

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Vaccination in otitis media. Keynote lecture2013

    • Author(s)
      Kodama S
    • Organizer
      20th IFOS(第20回世界耳鼻咽喉科学会.基調講演)
    • Place of Presentation
      Seoul
    • Year and Date
      2013-06-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 経鼻ワクチンによる免疫誘導2011

    • Author(s)
      児玉悟
    • Organizer
      第18回日本鼻科学会鼻科学会賞受賞講演.第50回日本鼻科学会
    • Place of Presentation
      岡山
    • Year and Date
      2011-12-02
    • Related Report
      2013 Final Research Report
  • [Presentation] 経鼻ワクチンによる免疫誘導2011

    • Author(s)
      児玉 悟
    • Organizer
      日本鼻科学会
    • Place of Presentation
      岡山市
    • Related Report
      2011 Research-status Report
  • [Presentation] Vaccination in otitis media.

    • Author(s)
      Kodama S.
    • Organizer
      20th IFOS(第20回世界耳鼻咽喉科学会. 基調講演).
    • Place of Presentation
      COEX Convention Center, Seoul, Korea
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 上気道粘膜免疫におけるNKT細胞の機能解析

    • Author(s)
      梅本真吾、児玉悟、野田謙二、鈴木正志
    • Organizer
      第31回日本耳鼻咽喉科免疫アレルギー学会
    • Place of Presentation
      倉敷市
    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.med.oita-u.ac.jp/ent/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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