Development of the novel approaches to diagnose and treat head and neck cancer using nanotechnology

• Project/Area Number: 23592520
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Iwate Medical University
• Principal Investigator: SHIGA Kiyoto 岩手医科大学, 医学部, 教授 (10187338)
• Co-Investigator(Kenkyū-buntansha): KODAMA Tetuya 東北大学, その他の研究科, 教授 (40271986)
• Co-Investigator(Renkei-kenkyūsha): MORI Shirou (80230069)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 頭頸部癌 / 頸部リンパ節転移 / 造影超音波診断 / 血管新生 / マイクロバブル / ナノバブル / 超音波診断 / 腫瘍血管 / 分子導入

Research Abstract

It is well known that angiogenic processes in growing tumors promote increased vessel density and structural abnormalities. Lymph node metastasis of the head and neck cancer also showed this neo-vascularization features in active phase of metastasis. The purpose of this study is to evaluate the vascular density and morphology in metastatic lymph nodes by using microbubbles as ultrasound contrast-enhancing agent. On the next stage, we intend to use hybrid nanobubbles to introduce some anti-tumor agents or transformed constructs into cancer cells as drug delivery system. We developed novel image-analyzing software to analyze capillary vessels in lymph nodes of the patients. After they underwent neck dissection, all of the patients who showed contrast-enhancement in their lymph nodes had carcinoma metastases in their lymph nodes by histopathological examination. Contrast ultrasound using our software is useful to detect lymph node metastasis of the head and neck cancer.

Research Products

• [Journal Article] Lymphatic mapping of mice with systemic lymphoproliferative disorder : Usefulness as an inter-lymph node metastasis model of cancer (2013)
  • Author(s): Lenan Shao, Shiro Mori, Yoko Yagishita, Tatsuki Okuno, Yuriko Hatakeyama, Takuma Sato, Tetsuya Kodama
  • Journal Title: J Immuno Methods Volume: 386 Pages: 69-73
• [Journal Article] Mouse Model of Lymph Node Metastasis via Afferent Lymphatic Vessels for Development of Imaging Modalities (2013)
  • Author(s): Li Li, Shiro Mori, Maya Sakamoto, Shoki Takahashi, Tetsuya Kodama.
  • Journal Title: PLOS ONE Volume: 8
• [Journal Article] Enhancedsonographic imaging to diagnose lymph node metastasis: importance of bloodvessel volume and density (2013)
  • Author(s): Li Li, Mori S, Kodama M, Sakamoto M, Takahashi S, KodamaT
  • Journal Title: Cancer Reseach Volume: 73 Issue: 7 Pages: 2082-2092
  • DOI: 10.1158/0008-5472.can-12-4200
  • Peer Reviewed
• [Journal Article] Lymphatic mapping of mice with systemic lymphoproliferative disorder: Usefulness as an inter-lymph node metastasis model of cancer. (2013)
  • Author(s): Lenan Shao, Shiro Mori, Yoko Yagishita, Tatsuki Okuno, Yuriko Hatakeyama, Takuma Sato, Tetsuya Kodama.
  • Journal Title: J Immuno Methods Volume: 389 Pages: 69-73
  • Peer Reviewed
• [Journal Article] Mouse Model of Lymph Node Metastasis via Afferent Lymphatic Vessels for Development of Imaging Modalities. (2013)
  • Author(s): Li Li, Shiro Mori, Maya Sakamoto, Shoki Takahashi, Tetsuya Kodama.
  • Journal Title: PLOS ONE Volume: 8
  • Peer Reviewed
• [Journal Article] Malignant melanoma of the head and neck : A multi-institutional retrospective analysis of cases in Northern Japan (2012)
  • Author(s): Shiga K, Ogawa T, Kobayashi T, Ueda S, Kondo A, Nanba A, Kuwashima S, Asada Y, Suzuki S, Nagahashi T, Takahashi M, Suzuki M, Ishida A, Watanabe K, Harabuchi Y, Himi T, Shinkawa H, Sato H, Saijyo S, Fukuda S, Tanaka K, Ishikawa K, Omori K, Aoyagi M, Hashimoto S
  • Journal Title: Head Neck Volume: (in press) Issue: 11 Pages: 1537-1541
  • DOI: 10.1002/hed.21984
  • Peer Reviewed
• [Journal Article] Difficulty of the differential diagnosis of basal cell adenoma and basal cell adenocarcinoma of parotid gland by magnetic resonance imaging: case reports and literature review (2012)
  • Author(s): Kojima I, Sakamoto M, Watanabe M,Takahashi S, Mugikura S, Shiga K, Iikubo M, Yamamura MJ, Sasano T.
  • Journal Title: Oral Radiol Volume: 28 Issue: 1 Pages: 24-31
  • DOI: 10.1007/s11282-011-0076-7
  • Peer Reviewed
• [Journal Article] miR-34a is downregulated in cis-diamminedichloroplatinum treated sinonasal squamous cell carcinoma patients with poor prognosis (2012)
  • Author(s): Takenori Ogawa, Yuriko Saiki, Kiyoto Shiga, Na Chen, Shinichi Fukushige, Makoto Sunamura, Hiroki Nagase, Sho Hashimoto, Kazuto Matsuura, Shigeru Saijo, Toshimitsu Kobayashi, Akira Horii
  • Journal Title: Cancer Sci Volume: 103 Pages: 1737-1743
  • Peer Reviewed
• [Journal Article] Differences between Oral Cancer and Cancer of the Pharynx & Larynx on Molecular level. (2012)
  • Author(s): Kiyoto Shiga, Takenori Ogawa, Katsunori Katagiri, Fumiaki Yoshida, Masaru Tateda, Toshimitsu Kobayashi.
  • Journal Title: Oncology Letters Volume: 3 Pages: 238-243
  • Peer Reviewed
• [Journal Article] Distinct Features of Second Primary Malignancies in Head and Neck Cancer Patients in Japan. (2011)
  • Author(s): Kiyoto Shiga, Masaru Tateda, Katsunori Katagiri, Ayako Nakanome, Takenori Ogawa, Yukinori Asada, Kengo Kato and Toshimitsu Kobayashi.
  • Journal Title: Tohoku J Exp Med Volume: 225 Pages: 5-12
  • Peer Reviewed
• [Journal Article] Concomitant chemo-radiotherapy as a standard treatment for squamous cell carcinoma of the temporal bone. (2011)
  • Author(s): Shiga K, Ogawa T, Maki A, Amano M, Kobayashi T.
  • Journal Title: Skull Base Volume: 21 Pages: 153-158
  • Peer Reviewed
• [Journal Article] Surgical treatment is recommended for advanced oral squamous cell carcinoma. (2011)
  • Author(s): Ogawa T, Matsuura K, Shiga K, Tateda M, Katagiri K, Kato K, Saijo S, Kobayashi T.
  • Journal Title: Tohoku J Exp Med Volume: 223 Pages: 17-25
  • Peer Reviewed
• [Presentation] Contrast ultrasound to detect lymph node metastasis of head and neck cancer (2013)
  • Author(s): Daisuke Saito, Kiyoto Shiga, Katsunori Katagiri, Shun Sagai
  • Organizer: EUROSON2013
  • Place of Presentation: Germany
• [Presentation] Contrast ultrasound to detect lymph node metastasis of head and neck cancer (2013)
  • Author(s): Daisuke Saito, Kiyoto Shiga, Katsunori Katagiri, Shun Sagai
  • Organizer: EUROSON2013
  • Place of Presentation: Stuttgart, Germany