Analysis of Head and Neck Cancer malignancy related to EMT and Cancer Stem Cells

• Project/Area Number: 23592521
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Otorhinolaryngology
• Research Institution: Tohoku University
• Principal Investigator: KATO KENGO 東北大学, 医学(系)研究科(研究院), 非常勤講師 (40455788)
• Co-Investigator(Kenkyū-buntansha): TANAKA Nobuyuki 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 部長 (60280872) ; MATSUURA Kazuto 地方独立法人宮城県立病院機構宮城県立がんセンター(研究所), がん先進治療開発研究部, 特任研究員 (70271947)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 癌 / 癌幹細胞 / EMT / 頭頸部癌 / ポリコム

Research Abstract

Malignant phenotype of Head and Neck Cancer (HNC) is related to EMT and polycomb gene induction, and emerging evidence suggests a role of EMT/Polycombs on Cancer stem cell (CSC) induction. In this study, we investigated whether EMT and polycomb genes control CSC of HNC cells. Forced expression of the Bmi1, EZH2 and Slug in HNC cell lines induced cancer stemness phenotypes, such as ALDH1 activity. Two inflammatory cytokines, TGFb and TNFa potentiated Bmi1-related CSC phenotypes. These results suggest that polycomb and EMT genes under inflammatory environment regulate malignant phenotypes of HNC.

Research Products

• [Journal Article] The role of initial neck dissection for patients with node-positive oropharyngeal squamous cell carcinomas (2014)
  • Author(s): T. Sakashita, A. Homma, R. Hayashi, K. Kawabata, K. Yoshino, S. Iwae, Y. Hasegawa, K. Nibu, T. Kato, K. Shiga, K. Matsuura, N. Monden, M. Fuji
  • Journal Title: Oral Oncology Volume: 40 Issue: 7 Pages: 211-215
  • DOI: 10.1016/j.oraloncology.2014.03.003
  • NAID: 120005464719
  • Peer Reviewed
• [Journal Article] Multi-institutional retrospective study for the evaluation of ocular function-preservation rates in maxillary sinus squamous cell carcinomas with orbital invasion (2014)
  • Author(s): T. Sakashita, R. Hayashi, A. Homma, K. Matsuura, K. Kato, K. Kawabata, N. Monden, Y. Hasegawa, T. Onitsuka, Y. Fujimoto, S. Iwae, K.Okami, T. Matsuzuka, K. Yoshino, M. Fujii
  • Journal Title: HEAD & NECK
  • NAID: 120005740326
  • Peer Reviewed
• [Journal Article] Hepatocyte Growth Factor Regulated Tyrosine Kinase Substrate in the peripheral development and function of B-cells (2014)
  • Author(s): Nagata T, Murata K, Murata R, Sun S-L, Saito Y, Yamaga S, Tanaka N, Tamai K, Moriya K, Kasai N, Sugamura K, and Ishii N.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 443 Issue: 2 Pages: 351-356
  • DOI: 10.1016/j.bbrc.2013.11.029
  • Peer Reviewed
• [Journal Article] Large noncoding RNA HOTAIR enhances aggressive biological behavior and is associated with short disease-free survival in human non-small cell lung cancer (2013)
  • Author(s): Nakagawa T, Endo H, Yokoyama M, Abe J, Tamai K, Tanaka N, Sato I, Takahashi S, Kondo T, Satoh K
  • Journal Title: Biochem Biophy Res Commun Volume: 436 Pages: 319-324
  • Peer Reviewed
• [Journal Article] 化学放射線療法に対する栄養管理・口腔ケア (2013)
  • Author(s): 加藤健吾、松浦一登
  • Journal Title: 耳鼻と臨床 Volume: 59巻Suppl.1 Pages: 8-13
  • NAID: 130004862310
• [Journal Article] 上顎洞原発扁平上皮癌T4症例の多施設による後ろ向き観察研究 (2013)
  • Author(s): 本間明宏,林隆一,松浦一登,加藤健吾,川端一喜,門田伸也,長谷川泰久,鬼塚哲郎,藤本保志,岩江法,大上研二,松塚崇,吉野邦俊,藤井正人
  • Journal Title: 頭頸部癌(1349-5747) Volume: 39 Pages: 310-316
  • NAID: 130004510170
• [Journal Article] 活動性の低下した高齢者に対し補中益気湯とアマンタジンが改善効果を示した2症例 (2013)
  • Author(s): 加藤健吾,松村治,菅野元,山崎克彦,齋藤文則,渡會恵介,菅野龍彦.
  • Journal Title: 漢方医学(0288-2485) Volume: 37 Pages: 232-234
  • NAID: 40019787760
• [Journal Article] CD271 Defines a Stem Cell-Like Population in Hypopharyngeal Cancer (2013)
  • Author(s): Imai T
  • Journal Title: PLoS One Volume: 8 Issue: 4 Pages: e62002-e62002
  • DOI: 10.1371/journal.pone.0062002
  • Peer Reviewed
• [Journal Article] Enhanced expression of long non-coding RNA HOTAIR is associated with the development of gastric cancer. (2013)
  • Author(s): Endo H, Shiroki T, Nakagawa T, Yokoyama M, Tamai K, Yamanami H, Fujiya T, Sato I, Yamaguchi K, Tanaka N, Iijima K, Shimosegawa T, Sugamura K, Satoh K.
  • Journal Title: PlosONE Volume: 8 Issue: 10 Pages: e77070-e77070
  • DOI: 10.1371/journal.pone.0077070
  • Peer Reviewed
• [Journal Article] Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ape1/Ref-1) is essential for IL-21-induced signal transduction through ERK1/2 pathway (2012)
  • Author(s): Juliana FM, Nara H, Onoda T, Rahman M, Araki A, Jin L, Fujii H, Tanaka N, Hoshino T, Asao H
  • Journal Title: Biochem Biophys Res Commun Volume: 420(3) Pages: 628-34
  • Peer Reviewed
• [Journal Article] Regulation of hepatitis C virus secretion by the Hrs-dependent exosomal pathway (2012)
  • Author(s): Tamai K, Shiina M, Tanaka N, Nakano T, Yamamoto A, Kondo Y, Kakazu E, Inoue J, Fukushima K, Sano K, Ueno Y, Shimosegawa T, Sugamura K
  • Journal Title: Virology Volume: 422(2) Pages: 377-85
  • Peer Reviewed
• [Journal Article] Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma (2012)
  • Author(s): Inoue D, Suzuki T, Mitsuishi Y, Miki Y, Suzuki S, Sugawara S, Watanabe M, Sakurada A, Endo C, Uruno A, Sasano H, Nakagawa T, Satoh K, Tanaka N, Kubo H, Motohashi H, Yamamoto M
  • Journal Title: Cancer Sci Volume: 103(4) Pages: 760-6
  • Peer Reviewed
• [Journal Article] Accumulation of p62/SQSTM1 is associated with poor prognosis in patients with lung adenocarcinoma (2012)
  • Author(s): Inoue D, Suzuki T, Mitsuishi Y, Miki Y, Suzuki S, Sugawara S, Watanabe M, Sakurada A, Endo C, Uruno A, Sasano H, Nakagawa T, Satoh K, Tanaka N, Kubo H, Motohashi H, Yamamoto M
  • Journal Title: Cancer Sci Volume: 103 Issue: 4 Pages: 760-766
  • DOI: 10.1111/j.1349-7006.2012.02216.x
  • Peer Reviewed
• [Journal Article] The AAA-ATPase VPS4 regulates extracellular secretion and lysosomal targeting ofα-synuclein (2011)
  • Author(s): Hasegawa T, Konno M, Baba T, Sugeno N, Kikuchi A, Kobayashi M, Miura E, Tanaka N, Tamai K, Furukawa K, Arai H, Mori F, Wakabayashi K, Aoki M, Itoyama Y, Takeda A
  • Journal Title: PLoS One Volume: 6(12)
  • Peer Reviewed
• [Journal Article] AMSH is required to degrade ubiquitinated proteins in the central nervous system (2011)
  • Author(s): Suzuki S, Tamai K, Watanabe M, Kyuuma M, Ono M, Sugamura K, Tanaka N
  • Journal Title: Biochem Biophys Res Commun Volume: 408(4) Pages: 582-8
  • Peer Reviewed
• [Journal Article] Hrs Recognizes a Hydrophobic Amino Acid Cluster in Cytokine Receptors during Ubiquitin-independent Endosomal Sorting (2011)
  • Author(s): Amano Y, Yamashita Y, Kojima K, Yoshino K, Tanaka N, Sugamura K, Takeshita T
  • Journal Title: J Biol Chem Volume: 286 Pages: 15458-72
  • NAID: 120007110518
  • Peer Reviewed
• [Journal Article] WSB-1, a novel IL-21 receptor binding molecule, enhances the maturation of IL-21 receptor (2011)
  • Author(s): Nara H, Onoda T, Rahman M, Araki A, Juliana FM, Tanaka N, Asao H
  • Journal Title: Cell Immunol Volume: 269(1) Pages: 54-9
  • Peer Reviewed
• [Journal Article] 経皮内視鏡的胃瘻造設術(PEG)を行った頭頸部癌患者の検討 (2011)
  • Author(s): 角田梨紗子,松浦一登,野口哲也,加藤健吾,片桐克則,今井隆之,石田英一,西條茂
  • Journal Title: 頭頸部癌(1349-5747) Volume: 37 Pages: 433-438
  • NAID: 10030815358
• [Journal Article] 嚥下障害に対して半夏厚朴湯とアマンタジンおよび嚥下訓練の併用療法が著効した1症例 (2011)
  • Author(s): 16.加藤健吾,松村治,太田信之介,菅野龍彦
  • Journal Title: 漢方医学 Volume: 35 Pages: 276-278
• [Journal Article] 当科における喉頭全摘術後の合併症の検討 (2011)
  • Author(s): 志賀清人,小川武則,加藤健吾
  • Journal Title: 喉頭 Volume: 23 Pages: 22-25
  • NAID: 10030273642
• [Journal Article] The AAA-A TPase VPS4 Regulates Extracellular Secretion and Lysosomal Targeting of alpha-Synuclein (2011)
  • Author(s): Hasegawa T, Konno M, Baba T, Sugeno N, Kikuchi A, Kobayashi M, Miura E, Tanaka N, Tamai K, Furukawa K, Arai H, Mori F, Wakabayashi K, Aoki M, Itoyama Y, Takeda A
  • Journal Title: PLoS One Volume: 6 Issue: 12 Pages: 1-14
  • DOI: 10.1371/journal.pone.0029460
  • Peer Reviewed
• [Journal Article] CD271 defines a stem cell-like population in hypopharyngeal cancer
  • Author(s): Imai, T.Tamai, K.Oizumi, S.Oyama, K.Yamaguchi, K.Sato, I.Satoh, K.Matsuura, Saijo, S.Sugamura, K. and Tanaka, N
  • Journal Title: PLoS ONE Volume: 8(4)
  • Peer Reviewed
• [Presentation] 下咽頭癌における新規がん幹細胞表面マーカー"CD271"の同定 (2013)
  • Author(s): 今井隆之、玉井恵一、山口壱範、佐藤郁郎、佐藤賢一、松浦一登、西條茂、菅村和夫、田中伸幸
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] 下咽頭癌における新規がん幹細胞表面マーカー”CD271”の同定
  • Author(s): 今井隆之、玉井恵一、山口壱範、佐藤郁郎、佐藤賢一、松浦一登、西條茂、菅村和夫、田中伸幸
  • Organizer: 第７２回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Book] 第VII章各種がんにおける栄養管理1.頭頸部がん.NST・緩和ケアチームのための癌栄養管理完全ガイド(比企直樹・土師誠二・向山雄人編) (2014)
  • Author(s): 松浦一登、加藤健吾
  • Publisher: 文光堂