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Development of molecular targeting therapy for diabetic retionopathy based on gene expression profiling of fibrovascular membranes

Research Project

Project/Area Number 23592574
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionKyushu University

Principal Investigator

YOSHIDA Shigeo  九州大学, 医学(系)研究科(研究院), 講師 (50363370)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Tatsuro  九州大学, 医学研究院, 教授 (30150428)
KONO Toshihiro  福岡大学, 医学部, 講師 (80580315)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsゲノムワイド遺伝子発現解析 / ペリオスチン / 増殖糖尿病網膜症 / 増殖硝子体網膜症 / 核酸医薬 / 糖尿病網膜症
Research Abstract

We found increased periostin expression in the vitreous of patients with proliferative diabetic retinopathy (PDR). Immunohistochemical analysis showed colocalization of periostin and a-SMA in PDR-ERMs. In vitro, periostin increased proliferation, adhesion, migration, and collagen production in RPE cells. Periostin blockade suppressed migration and adhesion induced by TGFb2. In vivo, periostin inhibition had the inhibitory effect on experimental retinal fibrovascular formartion without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for ERM formation as well as a promising therapeutic target for PDR.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (29 results)

All 2014 2013 2012 2011 Other

All Journal Article (10 results) (of which Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (18 results) (of which Invited: 5 results) Remarks (1 results)

  • [Journal Article] Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy2014

    • Author(s)
      Ishikawa K, Yoshida S, Nakao S, Nakama T, Kita T, Asato R, Sassa Y, Arita R, Miyazaki M, Enaida H, Oshima Y, Murakami N, Niiro H, Ono J, Matsuda A, Goto Y, Akashi K, Izuhara K, Kudo A, Kono T, Hafezi-Moghadam A, and Ishibashi T
    • Journal Title

      FASEB J

      Volume: 28 Pages: 131-142

    • Related Report
      2013 Final Research Report
  • [Journal Article] Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy2014

    • Author(s)
      Ishikawa K, Yoshida S, Nakao S, Nakama T, Kita T, Asato R, Sassa Y, Arita R, Miyazaki M, Enaida H, Oshima Y, Murakami N, Niiro H, Ono J, Matsuda A, Goto Y, Akashi K, Izuhara K, Kudo A, Kono T, Hafezi-Moghadam A, Ishibashi T.
    • Journal Title

      FASEB J

      Volume: 28 Issue: 1 Pages: 131-142

    • DOI

      10.1096/fj.13-229740

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes2013

    • Author(s)
      Asato R, Yoshida S, Ogura A, Nakama T, Ishikawa K, Nakao S, Sassa Y, Enaida H, Oshima Y, Ikeo K, Gojobori T, Kono T, and Ishibashi T
    • Journal Title

      PLoS One

      Volume: 8

    • NAID

      120005227330

    • Related Report
      2013 Final Research Report
  • [Journal Article] Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes2013

    • Author(s)
      Asato R, et. al.
    • Journal Title

      PLoS One.

      Volume: 8 Issue: 1 Pages: e54191-e54191

    • DOI

      10.1371/journal.pone.0054191

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antiangiogenic shift in vitreous after vitrectomy in patients with proliferative diabetic retinopathy2012

    • Author(s)
      Yoshida S, Nakama T, Ishikawa K, Arima M, Tachibana T, Nakao S, Sassa Y, Yasuda M, Enaida H, Oshima Y, Kono T, and Ishibashi T
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53 Pages: 6997-7003

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Bone marrow-derived monocyte lineage cells recruited by MIP-1beta promote physiological revascularization in mouse model of oxygen-induced retinopathy2012

    • Author(s)
      Ishikawa K, Yoshida S, Nakao S, Sassa Y, Asato R, Kohno R, Arima M, Kita T, Yoshida A, Ohuchida K, and Ishibashi T
    • Journal Title

      Lab Invest

      Volume: 92 Pages: 91-101

    • Related Report
      2013 Final Research Report 2012 Research-status Report
  • [Journal Article] Involvement of periostin in regression of hyaloidvascular system during ocular development2012

    • Author(s)
      Arima M, Yoshida S, Nakama T, Ishikawa K, Nakao S, Yoshimura T, Asato R, Sassa Y, Kita T, Enaida H, Oshima Y, Matsuda A, Kudo A, and Ishibashi T
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 53 Pages: 6495-6503

    • Related Report
      2013 Final Research Report
  • [Journal Article] Involvement of periostin in regression of hyaloidvascular system during ocular development2012

    • Author(s)
      Arima M, et al.
    • Journal Title

      Invest Ophthalmol Vis Sci.

      Volume: 53 Issue: 10 Pages: 6495-6503

    • DOI

      10.1167/iovs.12-9684

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Increased expression of periostin in vitreous and fibrovascular membranes obtained from patients with proliferative diabetic retinopathy2011

    • Author(s)
      Yoshida S, Ishikawa K, Asato R, Arima M, Sassa Y, Yoshida A, Yoshikawa H, Narukawa K, Obika S, Ono J, Ohta S, Izuhara K, Kono T, and Ishibashi T
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 52 Pages: 5670-5678

    • Related Report
      2013 Final Research Report
  • [Journal Article] Increased expression of periostin in vitreous and fibrovascular membranes obtained from patients with proliferative diabetic retinopathy2011

    • Author(s)
      Yoshida, S. Ishikawa, K. Asato, R. Arima, M. Sassa, Y. Yoshida, A. Yoshikawa, H. Narukawa, K. Obika, S. Ono, J. Ohta, S. Izuhara, K. Kono, T. Ishibashi, T.
    • Journal Title

      Invest Ophthalmol Vis Sci

      Volume: 52 Pages: 5670-8

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 糖尿病網膜症に対する分子標的治療2014

    • Author(s)
      吉田茂生
    • Organizer
      第24回やまがたDM meeting
    • Place of Presentation
      山形市
    • Year and Date
      2014-03-01
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病網膜症の薬物治療2014

    • Author(s)
      吉田茂生
    • Organizer
      第11回福島糖尿病眼研究会
    • Place of Presentation
      郡山市
    • Year and Date
      2014-02-08
    • Related Report
      2013 Final Research Report
  • [Presentation] ゲノム医科学的アプローチによる増殖性網膜硝子体疾患の責任遺伝子同定と治療への展開2013

    • Author(s)
      吉田茂生
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京都
    • Year and Date
      2013-04-06
    • Related Report
      2013 Final Research Report
  • [Presentation] 網膜硝子体サンプルが教えてくれる次世代医療へのヒント2012

    • Author(s)
      吉田茂生
    • Organizer
      第93回秋田県眼科集談会
    • Place of Presentation
      秋田市
    • Year and Date
      2012-12-09
    • Related Report
      2013 Final Research Report
  • [Presentation] The role of M2 macrophages in the development of fibrovascular membranes in diabetic retinopathy2012

    • Author(s)
      Yoshida S
    • Organizer
      The Fifth Joint Meeting of Korea-China-Japan Ophthalmologists
    • Place of Presentation
      Fukuoka
    • Year and Date
      2012-11-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Fibrovascular membranes associated with PDR : Identification of molecular targets by global gene expression profiling2012

    • Author(s)
      Yoshida Shigeo
    • Organizer
      The Third Kyushu-Youngnam Retina Meeting
    • Place of Presentation
      Busan
    • Year and Date
      2012-08-18
    • Related Report
      2013 Final Research Report
  • [Presentation] 遺伝性眼疾患の基本知識2011

    • Author(s)
      吉田茂生
    • Organizer
      第4回愛知県眼科医会学術講演会
    • Place of Presentation
      名古屋市
    • Year and Date
      2011-11-12
    • Related Report
      2013 Final Research Report
  • [Presentation] 糖尿病合併症の克服を目指して-基礎から臨床へ包括的遺伝子発現解析を用いた糖尿病網膜症に対する治療標的の抽出2011

    • Author(s)
      吉田茂生,石橋達朗
    • Organizer
      第49回日本糖尿病学会
    • Place of Presentation
      福岡市
    • Year and Date
      2011-10-14
    • Related Report
      2013 Final Research Report
  • [Presentation] Increased Expression of Periostin in Vitreous and Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy2011

    • Author(s)
      Yoshida S, Ishikawa K, Asato R, Sassa Y, Yoshikawa H, Izuhara K, Kono T, Ishibashi T
    • Organizer
      The Association for Research in Vision and Ophthalmology Annual Meeting
    • Place of Presentation
      Fort Laudardale
    • Related Report
      2013 Final Research Report
  • [Presentation] Increased Expression of Periostin in Vitreous and Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy2011

    • Author(s)
      Yoshida, S. Ishikawa, K. Asato, R. Sassa, Y. Yoshikawa, H. Izuhara, K. Kono, T. Ishibashi, T.
    • Organizer
      Association for Research in Vision and Ophthalmology
    • Place of Presentation
      Fort Laudardale, USA
    • Related Report
      2011 Research-status Report
  • [Presentation] 遺伝性眼疾患の基本知識2011

    • Author(s)
      吉田茂生
    • Organizer
      第4回愛知県眼科医会学術講演会(招待講演)
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] 糖尿病合併症の克服を目指して-基礎から臨床へ 包括的遺伝子発現解析を用いた糖尿病網膜症に対する治療標的の抽出2011

    • Author(s)
      吉田茂生、石橋達朗
    • Organizer
      第49回日本糖尿病学会(招待講演)
    • Place of Presentation
      福岡
    • Related Report
      2011 Research-status Report
  • [Presentation] ゲノム医科学的アプローチによる増殖性網膜硝子体疾患の責任遺伝子同定と治療への展開,第117回日本眼科学会総会

    • Author(s)
      吉田 茂生
    • Organizer
      第117回日本眼科学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 糖尿病網膜症の薬物治療

    • Author(s)
      吉田 茂生
    • Organizer
      第11回福島糖尿病眼研究会
    • Place of Presentation
      郡山市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ,糖尿病網膜症に対する分子標的治療

    • Author(s)
      吉田 茂生
    • Organizer
      第24回やまがたDM meeting
    • Place of Presentation
      山形市
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] ,Fibrovascular membranes associated with PDR: Identification of molecular targets by global gene expression profiling.

    • Author(s)
      Yoshida, S.
    • Organizer
      The Third Kyushu-Youngnam Retina Meeting
    • Place of Presentation
      Busan
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] The role of M2 macrophages in the development of fibrovascular membranes in diabetic retinopathy.

    • Author(s)
      Yoshida, S.
    • Organizer
      The Fifth Joint Meeting of Korea-China-Japan Ophthalmologists.
    • Place of Presentation
      Fukuoka
    • Related Report
      2012 Research-status Report
  • [Presentation] 網膜硝子体サンプルが教えてくれる次世代医療へのヒント.

    • Author(s)
      吉田 茂生
    • Organizer
      第93回秋田県眼科集談会
    • Place of Presentation
      秋田
    • Related Report
      2012 Research-status Report
    • Invited
  • [Remarks]

    • URL

      http://www.eye.med.kyushu-u.ac.jp/

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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