Effects of protease-activated receptors (PARs) on intracellular calcium dynamics of acinar cells in rat lacrimal glands.
| Project/Area Number |
23592585
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Iwate Medical University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
SAINO Tomoyuki 岩手医科大学, 医学部, 教授 (40305991)
SATOH Yoh-ichi 岩手医科大学, 医学部, 教授 (40118253)
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| Co-Investigator(Renkei-kenkyūsha) |
KUROSAKA Daijiro 岩手医科大学, 医学部, 教授 (20215099)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 涙腺 / プロテアーゼ活性化型受容体 / 細胞内カルシウムイオン / 共焦点レーザー顕微鏡 / RT-PCR / 外分泌 / calcium entry / カルシウムシグナリング / 涙液分泌機構 / カルシウム流入機構 |
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| Research Abstract |
Protease-activated receptors (PARs) are G-protein-coupled receptors, activated by proteolytic cleavage. Here, lacrimal gland acinar cell responses to PAR activation were examined, with respect to intracellular Ca2+ concentration ([Ca2+]i) dynamics. Only PAR2 mRNA was detected in these acinar cells. A PAR2-activating peptide (PAR2-AP) induced an increase in [Ca2+]i in these cells. The removal of extracellular Ca2+ and the use of Ca2+ channel blockers did not inhibit PAR2-AP-induced [Ca2+]i increases. The NO donor mimicked the effects of PAR2 in activating non-capacitative calcium entry (NCCE). However, both calyculin A and a low concentration of Gd3+ did not completely block the PAR2-AP-induced increase in [Ca2+]i. These findings indicated that PAR2 activation resulted primarily in Ca2+ mobilization from intracellular Ca2+ stores and that PAR2-mediated [Ca2+]i changes were mainly independent of IP3. We suggest that PAR2-AP differentially regulates both NCCE and CCE, predominantly NCCE.
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Report
(4 results)
Research Products
(53 results)
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[Journal Article] World's first telepathology experiments employing WINDS ultra-high-speed internet satellite, nicknamed "KIZUNA"2013
Author(s)
Sawai T, Uzuki M, Miura Y, Kamataki A, Matsumura T, Saito K, Kurose A, Osamura YR, Yoshimi N, Kanno H, Moriya T, Ishida Y, Satoh Y, Nakao M, Ogawa E, Matsuo S, Kasai H, Kumagai K, Motoda T, Hopson N
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Journal Title
J Pathol Inform
Volume: 4
Pages: 24-24
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