Differentiation to keratocyte from oral mucosal non-ephthelial cells
| Project/Area Number |
23592592
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | Tokyo Dental College |
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Principal Investigator |
SHIMAZAKI Jun 東京歯科大学, 歯学部, 教授 (40170930)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 口腔粘膜 / 間葉系幹細胞 / 神経堤細胞 / 角膜実質 / 分化誘導 |
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| Research Abstract |
To establish method of differentiation into keratocyte from oral mucosal non-epithelial cells. Oral mucosal non-epithelial cells were isolated from oral mucosal tissues, cultured with methyl cellulose from single cell, and analyzed ability of multi-differentiation to mesenchymal cells, neural cells, or keratocytes. Oral mucosal non-epithelial cells were estimated the expression of mesenchymal stem cell markers. Oral mucosal non-epithelial cells selected neural crest marker, CD56 and PDGFRa, were differentiated into osteoblasts, adipocytes, chondrocytes, or neural cells. Keratocan, a marker of keratocytes, were detected in oral mucosal non-epithelial cells expressed mesenchymal stem cells markers. These data suggested that oral mucosal non-epithelial cells would be expected to a potential cell source for regeneration of corneal stroma.
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Report
(4 results)
Research Products
(2 results)
