Establishment of new concepts in cone electroretinogram using transgenic rabbits

• Project/Area Number: 23592603
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Mie University
• Principal Investigator: KONDO MINEO 三重大学, 医学(系)研究科(研究院), 教授 (80303642)
• Co-Investigator(Kenkyū-buntansha): TERASAKI Hiroko 名古屋大学, 医学(系)研究科(研究院), 教授 (40207478) ; KACHI Shu 名古屋大学, 医学部附属病院, 講師 (30345904) ; KOMEIMA Keiichi 名古屋大学, 医学部附属病院, 講師 (40362256) ; UENO Shinji 名古屋大学, 医学(系)研究科(研究院), 助教 (80528670)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 網膜色素変性 / 網膜電図 / 動物モデル / トランスジェニック / 錐体 / ウサギ / ロドプシン

Research Abstract

The purpose of this study was to determine the contributions of cones and post- photoreceptoral neurons to the a-wave of the photopic ERGs in rhodopsin Pro347Leu transgenic (Tg) rabbits. Post-photoreceptral neural function was completely suppressed by intravitreal injections of CNQX and APB. We found that the percentage contribution of the cone photoreceptorsto the photopic ERG a-waves ranged from 54% to 75% in wild-type (WT) rabbits. In contrast, the percentage contribution of the cone photoreceptors in Tg rabbits ranged from 32% to 51%. These results suggest that the relative contribution of cone photoreceptors to the photopic ERG a-wave is smaller in retinas with inherited photoreceptor degeneration.

Research Products

• [Journal Article] Clinical Phenotype in Ten Unrelated Japanese Patients with Mutations in the EYS Gene (2014)
  • Author(s): Suto K, Hosono K, Takahashi M, Hirami Y, Arai Y, Nagase Y, Ueno S, Terasaki H, Minoshima S, Kondo M, Hotta Y.
  • Journal Title: Ophthalmic Genet. Volume: 35(1) Issue: 1 Pages: 25-34
  • DOI: 10.3109/13816810.2013.768673
  • Peer Reviewed
• [Journal Article] Autoantibodies to transient receptor potential cation channel, subfamily M, member 1 in a Japanese patient with melanoma-associated retinopathy (2014)
  • Author(s): Morita Y, Kimura K, Fujitsu Y, Enomoto A, Ueno S, Kondo M, Sonoda KH
  • Journal Title: Japanese Journal of Ophthalmology Volume: 58 Issue: 2 Pages: 166-171
  • DOI: 10.1007/s10384-013-0300-6
  • Peer Reviewed
• [Journal Article] Degeneration of retinal on bipolar cells induced by serum including autoantibody against TRPM1 in mouse model of paraneoplastic retinopathy. (2013)
  • Author(s): Ueno S, Nishiguchi KM, Tanioka H, Enomoto A, Yamanouchi T, Kondo M, Yasuma TR, Yasuda S, Kuno N, Takahashi M, Terasaki H.
  • Journal Title: PLoS One Volume: 8(11) Issue: 11 Pages: e81507-e81507
  • DOI: 10.1371/journal.pone.0081507
  • Peer Reviewed
• [Journal Article] Relationship between retinal layer thickness and focal macular electroretinogram components after epiretinal membrane surgery. (2013)
  • Author(s): Hibi N, Ueno S, Ito Y, Piao CH, Kondo M, Terasaki H.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54(12) Issue: 12 Pages: 7207-7214
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  • Peer Reviewed
• [Journal Article] Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene (2013)
  • Author(s): Nishiguchi KM, Tearle RG, Liu YP, Oh EC, Miyake N, Benaglio P, Harper S, Koskiniemi-Kuendig H, Venturini G, Sharon D, Koenekoop RK, Nakamura M, Kondo M, Ueno S, Yasuma TR, Beckmann JS, Ikegawa S, Matsumoto N, Terasaki H, Berson EL, Katsanis N, Rivolta C
  • Journal Title: Proc Natl Acad Sci U S A Volume: 110 Issue: 40 Pages: 16139-16144
  • DOI: 10.1073/pnas.1308243110
  • Peer Reviewed
• [Journal Article] Focal cone ERGs of rhodopsin Pro347Leu transgenic rabbits. (2013)
  • Author(s): Ueno S, Koyasu T, Kominami T, Sakai T, Kondo M, Yasuda S, Terasaki H
  • Journal Title: Vision Res. Volume: 91 Pages: 118-123
  • DOI: 10.1016/j.visres.2013.08.006
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• [Journal Article] Functional endothelial progenitor cells selectively recruit neurovascular protective monocyte-derived F4/80(+) /Ly6c(+) macrophages in a mouse model of retinal degeneration (2013)
  • Author(s): Fukuda S, Nagano M, Yamashita T, Kimura K, Tsuboi I, Salazar G, Ueno S, Kondo M, Kunath T, Oshika T, Ohneda O
  • Journal Title: Stem Cells Volume: 31 Issue: 10 Pages: 2149-2161
  • DOI: 10.1002/stem.1469
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• [Journal Article] Age- dependent alteration of intraocular soluble heparan sulfate levels and its implications for proliferative diabetic retinopathy (2013)
  • Author(s): Nishiguchi KM, Ushida H, Tomida D, Kachi S, Kondo M, Terasaki H
  • Journal Title: Mol Vis Volume: 19 Pages: 1125-1131
  • URL: http://www.molvis.org/molvis/v19/1125
  • Peer Reviewed
• [Journal Article] Uniform suspension of the clustered triamcinolone acetonide particle (2013)
  • Author(s): Sugimoto M, Kondo M, Horiguchi M
  • Journal Title: J Ophthalmol Volume: 2013 Pages: 315658-315658
  • DOI: 10.1155/2013/315658
  • Peer Reviewed
• [Journal Article] Peripheral capillary nonperfusion and full-field electroretinographic changes in eyes with frosted branch-like appearance retinal vasculitis (2013)
  • Author(s): Matsui Y, Tsukitome H, Uchiyama E, Wada Y, Yagi T, Matsubara H, Kondo M
  • Journal Title: Clin Ophthalmol Volume: 7 Pages: 137-140
  • DOI: 10.2147/opth.s40110
• [Journal Article] Influence of preoperative intravitreal bevacizumab on visual function in eyes with proliferative diabetic retinopathy. (2013)
  • Author(s): Ushida H
  • Journal Title: Ophthalmic Res. Volume: 49（１） Issue: 1 Pages: 30-36
  • DOI: 10.1159/000324135
  • Peer Reviewed
• [Journal Article] Focal cone ERGs of rhodopsin Pro347Leu transgenic rabbits (2013)
  • Author(s): Ueno S, Koyasu T, Kominami T, Sakai T, Kondo M, Yasuda S, Terasaki H
  • Journal Title: Vision Research Volume: 91 Pages: 118-123
  • Peer Reviewed
• [Journal Article] Focal macular electroretinograms after intravitreal injections of bevacizumab for age-related macular degeneration. (2012)
  • Author(s): Iwata E
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53(7) Issue: 7 Pages: 4185-4190
  • DOI: 10.1167/iovs.11-9335
  • Peer Reviewed
• [Journal Article] Transcorneal electrical stimulation promotes survival of photoreceptors and improves retinal function in rhodopsin P347L transgenic rabbits. (2012)
  • Author(s): Morimoto T
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 53 Issue: 7 Pages: 4254-4261
  • DOI: 10.1167/iovs.11-9067
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• [Journal Article] Real-time imaging of rabbit retina with retinal degeneration by using spectral-domain optical coherence tomography. (2012)
  • Author(s): Muraoka Y et al.
  • Journal Title: PLoS One Volume: 7 Issue: 4 Pages: e36135-e36135
  • DOI: 10.1371/journal.pone.0036135
  • Peer Reviewed
• [Journal Article] Presynaptic dystroglycan-pikachurin complex regulates the proper synaptic connection between retinal photoreceptor and bipolar cells (2012)
  • Author(s): Omori, et al
  • Journal Title: J Neurosci Volume: 32 Issue: 18 Pages: 6126-6137
  • DOI: 10.1523/jneurosci.0322-12.2012
  • Peer Reviewed
• [Journal Article] Two Novel Mutations in the EYS Gene Are Possible Major Causes of Autosomal Recessive Retinitis Pigmentosa in the Japanese Population. (2012)
  • Author(s): Nakanishi H, Ueno S, Yokoi T, Hikoya A, Fujita T, Zhao Y, Nishina S, Shin JP, Kim IT, Yamamoto S, Azuma N, Terasaki H, Sato M, Kondo M, Minoshima M, Hotta Y.
  • Journal Title: PLoS One Volume: 7(2) Issue: 2 Pages: e31036-e31036
  • DOI: 10.1371/journal.pone.0031036
  • Peer Reviewed
• [Journal Article] Photoreceptor and post-photoreceptoral contributions to photopic ERG a-wave in rhodopsin P347L transgenic rabbits. (2012)
  • Author(s): Hirota R
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 53(3) Issue: 3 Pages: 1467-1472
  • DOI: 10.1167/iovs.11-9006
  • Peer Reviewed
• [Journal Article] Clinical features of a Japanese case with Bothnia dystrophy (2012)
  • Author(s): Nojima K, Hosono K, Zhao Y, Toshiba T, Hikoya A, Asai T, Kato M. Kondo M, Minoshima S, Hotta Y
  • Journal Title: Ophthalmic Genet Volume: 33(2) Issue: 2 Pages: 83-88
  • DOI: 10.3109/13816810.2011.634877
  • Peer Reviewed
• [Journal Article] International Society For Clinical Electrophysiology of Vision. ISCEV standard for clinical multifocal electroretinography (mfERG) (2011 edition) (2012)
  • Author(s): Hood DC, Bach M, Brigell M, Keating D, Kondo M, Lyons JS, Marmor MF, McCulloch DL, Palmowski-Wolfe AM
  • Journal Title: Doc Ophthalmol Volume: 124 Issue: 1 Pages: 1-13
  • DOI: 10.1007/s10633-011-9296-8
  • Peer Reviewed
• [Journal Article] Short-interfering RNAs induce retinal degeneration via TLR3 and IRF3 (2012)
  • Author(s): Kleinman ME
  • Journal Title: Mol Ther Volume: 20 Issue: 1 Pages: 101-8
  • DOI: 10.1038/mt.2011.212
  • Peer Reviewed
• [Journal Article] Retinal remodeling. (2012)
  • Author(s): Jones BW
  • Journal Title: Jpn J Ophthalmol Volume: 56(4) Issue: 4 Pages: 289-306
  • DOI: 10.1007/s10384-012-0147-2
  • Peer Reviewed
• [Journal Article] Enhancement of ON-bipolar cell responses of cone electroretinograms in rabbits with the Pro347Leu rhodopsin mutation. (2011)
  • Author(s): Nishimura T, Machida S, Kondo M, Terasaki H, Yokoyama D, Kurosaka D.
  • Journal Title: Invest. Ophthalmol. Vis. Sci. Volume: 52(10) Issue: 10 Pages: 7610-7617
  • DOI: 10.1167/iovs.11-7611
  • Peer Reviewed
• [Journal Article] miR-124a is required for hippocampal axogenesis and retinal cone survival through Lhx2 suppression (2011)
  • Author(s): Sanuki R, Onishi A, Koike C, Muramatsu R, Watanabe S, Muranishi Y, Irie S, Uneo S, Koyasu T, Matsui R, Cherasse Y, Urade Y, Watanabe D, KondoM, Yamashita T, Furukawa T
  • Journal Title: Nat Neurosci Volume: 14 Issue: 9 Pages: 1125-1134
  • DOI: 10.1038/nn.2897
  • Peer Reviewed
• [Journal Article] Significant correlation between electroretinogram parameters and ocular vascular endothelial growth factor concentration in central retinal vein occlusion eyes (2011)
  • Author(s): Yasuda S, Kachi S, Kondo M, Ushida H, Uetani R, Terui T, Piao CH, Terasaki H
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 52 Issue: 8 Pages: 5737-5742
  • DOI: 10.1167/iovs.10-6923
• [Journal Article] Analysis of transcriptional regulatory pathways of photoreceptor genes by expression profiling of the Otx2-deficient retina (2011)
  • Author(s): Omori Y, Katoh K, Sato S, Muranishi Y, Chaya T, Onishi A, Minami T, Fujikado T, Furukawa T
  • Journal Title: PLoS ONE Volume: 6(5) Issue: 5 Pages: e19911-e19911
  • DOI: 10.1371/journal.pone.0019911
  • Peer Reviewed
• [Journal Article] Rebound of macular edema after intravitreal bevacizumab therapy in eyes with macular edema secondary to branch retinal vein occlusion (2011)
  • Author(s): Yasuda S, Kondo M, Kachi S, Ito Y, Terui T, Ueno S, Terasaki H
  • Journal Title: Retina Volume: 31 Issue: 6 Pages: 1075-1082
  • DOI: 10.1097/iae.0b013e318206cf4b
  • Peer Reviewed
• [Journal Article] Changes in areas of capillary nonper- fusion after intravitreal injection of bevacizumab in eyes with branch retinal vein occlusion (2011)
  • Author(s): Terui T, Kondo M, Sugita T, Ito Y, Kondo N, Ota I, Miyake K, Terasaki H
  • Journal Title: Retina Volume: 31 Issue: 6 Pages: 1068-1074
  • DOI: 10.1097/iae.0b013e31820c83c2
  • Peer Reviewed
• [Journal Article] Autoantibodies to transient receptor potential cation channel, subfamily M, member 1 in a Japanese patient with melanoma-associated retinopathy
  • Author(s): Morita Y, Kimura K, Fujitsu Y, Enomoto A, Ueno S, Kondo M, Sonoda KH
  • Journal Title: Jpn J Ophthalmol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Transient increase of retinal nerve fiber layer thickness after macular hole surgery
  • Author(s): Hibi N, Kondo M, Ishikawa K, Ueno S, Komeima K, Terasaki H
  • Journal Title: Int Ophthalmol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Clinical Phenotype in Ten Unrelated Japanese Patients with Mutations in the EYS Gene
  • Author(s): Suto K, Hosono K, Takahashi M, Hirami Y, Arai Y, Nagase Y, Ueno S, Terasaki H, Minoshima S, Kondo M, Hotta Y
  • Journal Title: Jpn J Ophthalmol Volume: (in press)
  • Peer Reviewed
• [Presentation] A naturally occurring canine model of autosomal Recessive complete-type congenital stationary night blindness (2013)
  • Author(s): Kondo M, Imai R, Nakashita T, Imawaka M, Ueda K, Ohtsuka H, Sakai K, Matsubara H, Sugimoto M, Ueno S, Nishizawa Y, Das G, Miyadera K, Aguirre G
  • Organizer: Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Seatle, USA
• [Presentation] Photo- receptor and post-photoreceptoral contributions to photopic ERG a-wave in rhodopsin P347L transgenic rabbits (2012)
  • Author(s): Kondo M, Hirota R, Ueno S, Sakai T, Koyasu T, Terasaki H
  • Organizer: International Society for Electrophysiology of Vision
  • Place of Presentation: Quebec, Canada
  • Year and Date: 2012-06-11
• [Presentation] Retinal degeneration in Oguchi disease with SAG mutations (2012)
  • Author(s): Kondo M, Ueno S, Terasaki H, Hayashi T, Ishigami C, Takahashi M
  • Organizer: International Society of Clinical Electrophysiology of Vision
  • Place of Presentation: Valencia, Spain
• [Presentation] Identification of autoanti- bodies against TRPM1 in patients with paraneoplastic retinopathy associated with ON bipolar cell dysfunction (2011)
  • Author(s): Kondo M, Sanuki R, Ueno S, Nishizawa Y, Hashimoto N, Ohguro H, Yamamoto S, Machida S, Terasaki H, Adamus G, Furukawa T
  • Organizer: Annual Meeting of the Association for Research in Vision and Ophthalmology
  • Place of Presentation: Florida, USA
  • Year and Date: 2011-05-03
• [Presentation] Photoreceptor and Post-Photoreceptoral Contributions to Photopic ERG a-Wave in Rhodopsin P347L Transgenic Rabbits (2011)
  • Author(s): Kondo M, Hirota R, Ueno S, Sakai T, Koyasu T, Terasaki H
  • Organizer: International Society for Clinical Electrophysiology of Vision
  • Place of Presentation: Quebec City
• [Book] 「若年網膜分離症」眼科疾患最新の治療 (2013)
  • Author(s): 近藤峰生
  • Total Pages: 192
  • Publisher: 南江堂
• [Remarks]
  • URL: http://www.medic.mie-u.ac.jp/ophthalmology/