Development of a Rat Model for Retrobulbar Angiography of X-Ray Computed Tomography with an Iodine-Containing Polymer

• Project/Area Number: 23592605
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Osaka University
• Principal Investigator: MATSUSHITA Kenji 大阪大学, 医学(系)研究科(研究院), 講師 (40437405)
• Co-Investigator(Kenkyū-buntansha): 三木 篤也 大阪大学, 助教 (30437404)
• Co-Investigator(Renkei-kenkyūsha): 福山 直人 東海大学, 準教授 (50349338) ; 静間 徹 東海大学, 専任講師 (20307523)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 医用工学 / 放射光微小血管造影法 / 緑内障 / 眼医工学 / 放射光

Research Abstract

Recent adavances in optical coherence tomography allow visualization of the optic nerve structure. However, the infrared light source is limited in its ability to analyze the retrobulbar structure because of insufficient penetration; in contrast, X-rays can penetrate deeper. We developed a model for retrobulbar angiography with computed tomography to reveal the retrobulbar structures around the optic nerve head. Four-week-old SD rats were observed alive with CT. Trough the catheter, we had injected a contrast agent, iohexol and an iodine-containing polymer solution. After contrast agent washout, the CT values were recorded at baseline, maximal contrast agent enhancement, washout, and maximal iodine-containing polymer enhancement. We localized the area governed by ICA. The polymer stayed in the eye after the contrast was flushed from the orbit. We developed a model for retrobulbar angiography that makes it easy to adjust the imaging conditions.

Research Products

• [Journal Article] Circle- and grid-wise analyses of peripapillary nerve fiber layers by spectral domain optical coherence tomography in early-stage glaucoma (2013)
  • Author(s): Mayama C,Saito H, Hirasawa H, Konno S, Tomidokoro A, Araie M, Iwase A, Ohkubo S, Sugiyama K, Otani T, Kishi S, Matsushita K, Maeda N, Hangai M, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54(7) Pages: 4519-26
• [Journal Article] A case of aniridia with a heterozygous PAX6 mutation whose pituitary function was partially impaired (2013)
  • Author(s): Shimo N, Yasuda T, Kitamura T, Matsushita K, Osawa S, Yamamoto Y, Kozawa J, Otsuki M, Funahashi T, Imagawa A, Kaneto H, Nishida K, Shimomura I
  • Journal Title: Internal medicine Volume: (on publication)
• [Journal Article] Interface fluid syndrome after laser in situ keratomileusis following herpetic keratouveitis (2013)
  • Author(s): Goto S, Koh S, Toda R, Soma T, Matsushita K, Maeda N, Nishida K
  • Journal Title: J Cataract Refract Surg Volume: 39(8) Pages: 1267-70
• [Journal Article] Reproducibility of Thickness Measurements of Macular Inner Retinal Layers Using SD-OCT with or without Correction of Ocular Rotation (2013)
  • Author(s): Hirasawa H, Araie M, Tomidokoro A, Saito H, Iwase A, Ohkubo S, Sugiyama K, Ootani T, Kishi S, Matsushita K, Maeda N, Hangai M, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54(4) Pages: 2562-70
• [Journal Article] Circle- and grid-wise analyses of peripapillary nerve fiber layers by spectral domain optical coherence tomography in early-stage glaucoma. (2013)
  • Author(s): Mayama C,Saito H, Hirasawa H, Konno S, Tomidokoro A, Araie M, Iwase A, Ohkubo S, Sugiyama K, Otani T, Kishi S, Matsushita K, Maeda N, Hangai M, Yoshimura N.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54 Pages: 4519-26
  • Peer Reviewed
• [Journal Article] Reproducibility of Thickness Measurements of Macular Inner Retinal Layers Using SD-OCT with or without Correction of Ocular Rotation (2013)
  • Author(s): Hirasawa H, Araie M, Tomidokoro A, Saito H, Iwase A, Ohkubo S, Sugiyama K, Ootani T, Kishi S, Matsushita K, Maeda N, Hangai M, Yoshimura N
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 54 Pages: 2562-70
  • Peer Reviewed
• [Journal Article] Intraocular pressure elevation is a delayed-onset complication after successful vitrectomy for stages 4 and 5 retinopathy of prematurity (2012)
  • Author(s): Iwahashi-Shima C, Miki A, Hamasaki T, Otori Y, Matsushita K, Kiuchi Y, Okada M, Kusaka S
  • Journal Title: Retina Volume: 32(8) Pages: 1636-1642
• [Journal Article] Clinical outcomes and changes in aqueous vascular endothelial growth factor levels after intravitreal bevacizumab for iris neovascularization and neovascular glaucoma : a retrospective two-dose comparative study (2012)
  • Author(s): Sasamoto Y, Oshima Y, Miki A, Wakabayashi T, Song D, Matsushita K, Hamasaki T, Nishida K
  • Journal Title: J Ocul Pharmacol Ther Volume: 28(1) Pages: 41-8
• [Journal Article] Evaluation of the choroidal thickness using high-penetration optical coherence tomography with long wavelength in highly myopic normal-tension glaucoma (2012)
  • Author(s): Usui S, Ikuno Y, Miki A, Matsushita K, Yasuno Y, Nishida K
  • Journal Title: Am J Ophthalmol Volume: 153(1)
  • NAID: 120007137336
• [Journal Article] 良好な近見視力を示した黄斑低形成の一例 (2012)
  • Author(s): 藤本聡子、松下賢治、森本壮、不二門尚、西田幸二
  • Journal Title: 眼科臨床紀要 Volume: 5(6) Pages: 561-7
  • NAID: 40019356483
• [Journal Article] Intraocular Pressure Elevation Is a Delayed-onset Complication after Successful Vitrectomy for Stages 4 and 5 Retinopathy of Prematurity (2012)
  • Author(s): Iwahashi-Shima C, Miki A, Hamasaki T, Otori Y, Matsushita K, Kiuchi Y, Okada M, Kusaka S.
  • Journal Title: Retina Volume: 32(8) Issue: 8 Pages: 1636-42
  • DOI: 10.1097/iae.0b013e3182551c54
  • Peer Reviewed
• [Journal Article] Clinical outcomes and changes in aqueous vascular endothelial growth factor levels after intravitreal bevacizumab for iris neovascularization and neovascular glaucoma: a retrospective two-dose comparative study (2012)
  • Author(s): Sasamoto Y, Oshima Y, Miki A, Wakabayashi T, Song D, Matsushita K, Hamasaki T, Nishida K
  • Journal Title: J Ocul Pharmacol Ther. Volume: 28 Issue: 1 Pages: 41-8
  • DOI: 10.1089/jop.2011.0059
  • Peer Reviewed
• [Journal Article] Evaluation of the choroidal thickness using high-penetration optical coherence tomography with long wavelength in highly myopic normal-tension glaucoma. (2012)
  • Author(s): Usui S, Ikuno Y, Miki A, Matsushita K, Yasuno Y, Nishida K.
  • Journal Title: Am J Ophthalmol. Volume: 153 Pages: 10-16
  • NAID: 120007137336
  • Peer Reviewed
• [Journal Article] Effects of age, sex, and axial length on the three-dimensional profile of normal macular layer structures (2011)
  • Author(s): Ooto S, Hangai M, Tomidokoro A, Saito H, Araie M, Otani T, Kishi S, Matsushita K, Maeda N, Shirakashi M, Abe H, Ohkubo S, Sugiyama K, Iwase A, Yoshimura N
  • Journal Title: Ophthalmol Vis Sci Volume: 52(12) Pages: 8769-79
• [Journal Article] One-year results of intravitreal bevacizumab as an adjunct to trabeculectomy for neovascular glaucoma in eyes with previous vitrectomy (2011)
  • Author(s): Miki A, Oshima Y, Otori Y, Matsushita K, Nishida K
  • Journal Title: Eye (Lond) Volume: 25(5) Pages: 658-9
• [Journal Article] One-year results of intravitreal bevacizumab as an adjunct to trabeculectomy for neovascular glaucoma (2011)
  • Author(s): Miki A, Oshima Y, Otori Y, Matsushita K, Nishida K.
  • Journal Title: Eye Volume: 25(5): Pages: 658-9
  • Peer Reviewed
• [Journal Article] Effects of age, sex, and axial length on the three-dimensional profile of normal macular layer structures. (2011)
  • Author(s): Ooto S, Hangai M, Tomidokoro A, Saito H, Araie M, Otani T, Kishi S, Matsushita K,Maeda N, Shirakashi M, Abe H, Ohkubo S, Sugiyama K, Iwase A, Yoshimura N.
  • Journal Title: Invest Ophthalmol Vis Sci. Volume: 52 Pages: 8769-79
  • Peer Reviewed
• [Presentation] EXPRESSION OF AQUA/AQUAGLYCEROPORINS IN MURINE OPTIC NERVE HEAD ASTROCYTES (2014)
  • Author(s): Rumi Kawashima, Kenji Matsushita, Kohji Nishida
  • Organizer: ARVO
  • Place of Presentation: 米国オーランドコンベンションセンター
• [Presentation] ヨード含有ポリマー併用によるX線CT視神経乳頭造影法に対するラットモデルの作成 (2013)
  • Author(s): 松下賢治、河嶋瑠美、桑村里佳、臼井審一、盛英三、西田幸二
  • Organizer: 日本緑内障学会
  • Place of Presentation: 東京京王プラザ
• [Presentation] 培養視神経乳頭アストロサイトによる乳頭シンチウム形成の検討 (2013)
  • Author(s): 松下賢治
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] マウス視神経乳頭アストロサイトの単離培養法の確立 (2013)
  • Author(s): 河嶋瑠美
  • Organizer: 日本眼科学会
  • Place of Presentation: 東京
• [Presentation] Development of the model for rat retrobalbar angiography of X-ray CT with iode-containing polymer. (2013)
  • Author(s): 松下賢治
  • Organizer: ARVO
  • Place of Presentation: シアトルコンベンションセンター
• [Presentation] Functional Analysis of Cultured Astrocytes from the Murine Optic Nerve Head (2013)
  • Author(s): 河嶋瑠美
  • Organizer: ARVO
  • Place of Presentation: シアトルコンベンションセンター
• [Presentation] Development of the model for rat retrobulbar angiography of X ray CT with iode-containing polymer
  • Author(s): Matsushita K, Kawashima R Nishida K
  • Organizer: ARVO2013
• [Presentation] Functional analysis of cultured astrocytes from the murine optic nerve head
  • Author(s): Kawashima R, Matsushita K, Nishida K
  • Organizer: ARVO2013
• [Presentation] ヨード含有ポリマー併用によるX線CT視神経乳頭造影法に対するラットモデルの作成
  • Author(s): 松下賢治、河嶋瑠美、桑村里佳、臼井審一、盛英三、西田幸二
  • Organizer: 日本緑内障学会2013
• [Presentation] 培養視神経乳頭アストロサイトによる乳頭シンチウム形成の検討
  • Author(s): 松下賢治、河嶋瑠美、西田幸二
  • Organizer: 日本眼科学会2013
• [Presentation] マウス視神経乳頭アストロサイトの単離培養
  • Author(s): 河嶋瑠美松下賢治西田幸二
  • Organizer: 日本眼科学会2013
• [Book] 緑内障診断学における生体計測の進歩 (3夏号) (2012)
  • Author(s): 松下賢治
  • Total Pages: 64
  • Publisher: 生産と枝術