Analysis of Krt12 gene expression mechanism

Research Project

Project/Area Number	23592608
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Ophthalmology
Research Institution	Ehime University
Principal Investigator	OHASHI Yuichi 愛媛大学, 医学(系)研究科(研究院), 教授 (00116005)
Co-Investigator(Kenkyū-buntansha)	HAYASHI Yasuhito 愛媛大学, 医学部附属病院, 講師 (70314953)
Project Period (FY)	2011 – 2013
Project Status	Completed (Fiscal Year 2013)
Budget Amount *help	¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000) Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000) Fiscal Year 2012: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	K12 / 角膜上皮 / 幹細胞 / stem cell / K12遺伝子 / 遺伝子組換えマウス / バイオイメージング
Research Abstract	A mouse line, which has green fluorescent protein expression in K12 gene expressed cells, was established in this study. In this mouse cornea, we found corneal epithelial stem cells in basal layer of corneal epithelia. Farthermore, we examined CpG methylation pattern analysis between K12 promoter region and exon2.Compare to K12 non-expression cells, K12 expressed cells have less methylated CpG in CpG island and exon1 of the K12 gene.

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(4 results)