Roles of miRNA in the development of ocular inflammatory diseases

• Project/Area Number: 23592609
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Ophthalmology
• Research Institution: Kochi University
• Principal Investigator: NISHIUCHI TAKASHI 高知大学, 教育研究部医療学系, 助教 (90423475)
• Co-Investigator(Kenkyū-buntansha): FUKUSHIMA Atsuki 高知大学, 教育研究部医療学系, 教授 (40281737)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 眼炎症 / アレルギー性結膜炎 / マイクロＲＮＡ / ocular inflammation / allergic conjunctivitis / microRNA / アレルギー・ぜんそく / 遺伝子 / 免疫学

Research Abstract

Balb/c mice were immunized with ragweed in alum. Ten days later, the mice were challenged with ragweed in eye drops to induce allergic conjunctivitis. Twenty-four hours after the challenge, conjunctivas were harvested to extract RNA. As a control, conjunctivas were collected from immunized mice just prior to ragweed challenge. Expression of miRNA were compared between the two groups by using microarray analysis. Forty-four miRNAS were identified to be downregulated to be less than half by induction of conjunctivitits, while 74 miRNAS were identified to be upregulated to be more than 2-fold by induction of conjunctivitis.
Combined evaluation identified the target genes of miRNAS such as Lrp2 binding protein and lactoperoxidase.

Research Products

• [Journal Article] Oral administration of Ag suppresses Ag-induced allergic conjunctivitis in mice : critical timing and dose of Ag (2013)
  • Author(s): Ishida W, Fukuda Y, Taguchi O, Tsuda M, Y agita H, Fukushima A
  • Journal Title: Br J Oph thalmol Volume: 97(4) Pages: 492-497
  • Peer Reviewed
• [Journal Article] Behavior of bone marrow-derived cells following in vino transplantation : differenti ation into stromal cells with roles in organ maintenance (2013)
  • Author(s): Taguchi O, Tsujimura K ,Kontani K ,Hara da Y, Nomura S, Ikeda H, Morita A, Sugiur a H, Hayashi N, Yatabe Y, Seto M, Tatemat su M, Takahashi T, Fukushima A
  • Journal Title: Am J Pathol Volume: 182(4) Pages: 1255-1262
  • Peer Reviewed
• [Journal Article] Rebamipide increases barri sr function and attenuales TNF-αin duced barrier disruption and cytokyin e expression in human corneal epithel ial cells (2013)
  • Author(s): Tanaka H, Fukuda K, Ishida W, Harada Y, Fukushima A
  • Journal Title: Br J Ophthalmol Volume: 97(7) Pages: 912-916
  • Peer Reviewed
• [Journal Article] Alarmi ns from corneal epithelial cells up-r egulate CCLll and VCAM-1 in corneal f ibroblasts (2013)
  • Author(s): Fukuda K, Ishida W, Tanaka H, Harada Y, Matsuda A, Ebihara N, Fukushima A
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54(8) Pages: 5817-5823
  • Peer Reviewed
• [Journal Article] Identif ication of keratocyte-like cells diff erentiated from circulating bone marrow-derived cells in the mouse cornea (2013)
  • Author(s): Harada Y, Ishida W, Fukuda K, Sumi T, Kawakita T, Taguchi O, Fukushima A
  • Journal Title: Med Mol Morphol Volume: 46 Pages: 233-238
  • Peer Reviewed
• [Journal Article] Oral administration of Ag suppresses Ag-induced allergic conjunctivitis in mice: critical timing and dose of Ag. (2013)
  • Author(s): Ishida W, Fukuda K, Harada Y, Sumi T, Taguchi O, Tsuda M, Yagita H, Fukushima A.
  • Journal Title: Br J Ophthalmol Volume: 97(4) Issue: 4 Pages: 492-497
  • DOI: 10.1136/bjophthalmol-2012-302639
  • Peer Reviewed
• [Journal Article] Behavior of bone marrow-derived cells following in vivo transplantation: differentiation into stromal cells with roles in organ maintenance. (2013)
  • Author(s): Taguchi O, Tsujimura K, Kontani K, Harada Y, Nomura S, Ikeda H, Morita A, Sugiura H, Hayashi N, Yatabe Y, Seto M, Tatematsu M, Takahashi T, Fukushima A.
  • Journal Title: Am J Pathol Volume: 182(4) Issue: 4 Pages: 1255-1262
  • DOI: 10.1016/j.ajpath.2012.12.034
  • Peer Reviewed
• [Journal Article] Rebamipide increases barrier function and attenuates TNF-α-induced barrier disruption and cytokine expression in human corneal epithelial cells. (2013)
  • Author(s): Tanaka H, Fukuda K, Ishida W, Harada Y, Sumi T, Fukushima A.
  • Journal Title: Br J Ophthalmol Volume: 97(7) Issue: 7 Pages: 912-916
  • DOI: 10.1136/bjophthalmol-2012-302868
  • Peer Reviewed
• [Journal Article] Alarmins from corneal epithelial cells up-regulate CCL11 and VCAM-1 in corneal fibroblasts. (2013)
  • Author(s): Fukuda K, Ishida W, Tanaka H, Harada Y, Matsuda A, Ebihara N, Fukushima A.
  • Journal Title: Invest Ophthalmol Vis Sci Volume: 54(8) Issue: 8 Pages: 5817-5823
  • DOI: 10.1167/iovs.13-11969
  • Peer Reviewed
• [Journal Article] Identification of keratocyte-like cells differentiated from circulating bone marrow-derived cells in the mouse cornea. (2013)
  • Author(s): Harada Y, Ishida W, Fukuda K, Sumi T, Kawakita T, Taguchi O, Fukushima A.
  • Journal Title: Med Mol Morphol Volume: 46 Issue: 4 Pages: 233-238
  • DOI: 10.1007/s00795-013-0031-2
  • Peer Reviewed
• [Journal Article] High expression of nuclear factor 90 (NF90) leads to mitochondrial degradation in skel etal and cardiac muscles (2012)
  • Author(s): Higuchi T, Sakamoto S, Kakinuma Y, Ka i S, Yagyu K, Todaka H, Chi E, Okada S, Ujihara T, Morisawa K, Ono M, Sugi yama Y, Ishida W, Fukushima A, Tsuda M, Agata Y, Taniguchi T
  • Journal Title: PLoS One Volume: 7(8)
  • Peer Reviewed
• [Journal Article] Dynamic changes of microRNAs in the eye during the development of experimental autoimmune uveoretinitis (2011)
  • Author(s): Ishida W, Fukuda K, Higuchi T, Kajisa ko M, Sakamoto S, Fukushima A
  • Journal Title: Invest Ophth almol Vis Sci Volume: 52 Pages: 611-7
  • Peer Reviewed