| Budget Amount *help |
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Research Abstract |
Balb/c mice were immunized with ragweed in alum. Ten days later, the mice were challenged with ragweed in eye drops to induce allergic conjunctivitis. Twenty-four hours after the challenge, conjunctivas were harvested to extract RNA. As a control, conjunctivas were collected from immunized mice just prior to ragweed challenge. Expression of miRNA were compared between the two groups by using microarray analysis. Forty-four miRNAS were identified to be downregulated to be less than half by induction of conjunctivitits, while 74 miRNAS were identified to be upregulated to be more than 2-fold by induction of conjunctivitis.
Combined evaluation identified the target genes of miRNAS such as Lrp2 binding protein and lactoperoxidase.
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