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Development of ocular irritancy test using cultured corneal epithelial sheet

Research Project

Project/Area Number 23592611
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Ophthalmology
Research InstitutionTottori University (2013)
Tokyo Institute of Technology (2012)
Yokohama City University (2011)

Principal Investigator

ITO Norihiko  鳥取大学, 農学部, 准教授 (80264654)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Takahiro  同志社大学, 生命医科学部, 准教授 (30411078)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Keywords眼刺激性試験 / 動物実験代替法 / 培養角膜上皮細胞 / 再生医学 / ドレイズ試験 / 眼科学 / 獣医眼科学 / 再生医療 / 眼科 / 獣医眼科 / 角膜 / 培養角膜上皮 / 幹細胞 / 組織幹細胞 / 角膜毒性 / 角膜上皮細胞毒性
Research Abstract

The in vivo Draize eye test, which has become the international standard assay for eye irritation is often criticized for both ethical and scientific reasons. The 7th amendment of the Cosmetic directive will lead to the ban of animal testing for cosmetic ingredients. Thus, alternative strategies are necessary which allow the testing of chemicals with wide physicochemical properties under conditions similar to in vivo exposure.
We recently created an experimental model of cell damage repair closer to the live body than conventional models, by using layered sheets of cultured corneal epithelium. The corneal epithelial defect was repaired in the ophthalmic MFLX solution treatment and the ophthalmic LVFX solution treatment to a degree similar to that in the negative control group. Furthermore, we have developed more convenient test that enable us to perform high through put eye irritancy test.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (15 results)

All 2014 2013 2012 2011

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (12 results) (of which Invited: 2 results) Patent(Industrial Property Rights) (1 results) (of which Overseas: 1 results)

  • [Journal Article] Comparison of moxifloxacin and levofloxacin in an epithelial disorder model using cultured rabbit corneal epithelial cell sheets2012

    • Author(s)
      Miyake, T., N. Ito, et al
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: 250(7) Pages: 1035-1041

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Comparison of moxifloxacin and levofloxacin in an epithelial disorder model using cultured rabbit corneal epithelial cell sheets2012

    • Author(s)
      Taku Miyake, Norihiko Ito, Kazuki Tajima, Hiroshi Goto, Toshinori Furukawa
    • Journal Title

      Graefes Arch Clin Exp Ophthalmol

      Volume: in press Issue: 7 Pages: 1035-1041

    • DOI

      10.1007/s00417-011-1916-1

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 原子間力顕微鏡と再生医療材料である培養細胞を用いた薬剤評価方法の開発2014

    • Author(s)
      関禎子, (計7名)
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都国際会議場(京都市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 原子間力顕微鏡を用いた角膜上皮・内皮細胞の観察、及び薬剤の評価方法の開発2014

    • Author(s)
      関禎子, (計7名)
    • Organizer
      角膜カンファランス2014
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 原子間力顕微鏡を用いた角膜上皮・内皮細胞の観察、及び薬剤の評価方法の開発2014

    • Author(s)
      関禎子、月村亘、関宏也、伊藤典彦、田島一樹、高橋広樹、後藤浩
    • Organizer
      角膜カンファランス2014
    • Place of Presentation
      沖縄コンベンションセンター(宜野湾市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 原子間力顕微鏡と再生医療材料である培養細胞を用いた薬剤評価方法の開発2014

    • Author(s)
      関禎子、月村亘、関宏也、伊藤典彦、田島一樹、高橋広樹、後藤浩
    • Organizer
      第13回日本再生医療学総会
    • Place of Presentation
      京都国際会議場(京都市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 再生から創成2013

    • Author(s)
      伊藤典彦
    • Organizer
      鶴田フォーラム
    • Place of Presentation
      東工大蔵前会館(東京都)
    • Year and Date
      2013-12-14
    • Related Report
      2013 Final Research Report
  • [Presentation] 原子間力顕微鏡を用いた3次元皮膚モデルの評価方法の検討2013

    • Author(s)
      関禎子, (計8人)
    • Organizer
      第26回日本動物実験代替法学会
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2013 Final Research Report
  • [Presentation] AFMによる細胞培養基質の解析2013

    • Author(s)
      関禎子, (計6名)
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      タワーホール船橋(東京都)
    • Related Report
      2013 Final Research Report
  • [Presentation] 再生医療、動物から人へ、人から動物へ2013

    • Author(s)
      伊藤典彦
    • Organizer
      第34回動物臨床医学会総会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Related Report
      2013 Final Research Report
  • [Presentation] 再生医療、動物から人へ、人から動物へ2013

    • Author(s)
      伊藤典彦
    • Organizer
      第34回動物臨床医学会総会
    • Place of Presentation
      大阪国際会議場(大阪市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] AFMによる細胞培養基質の解析2013

    • Author(s)
      関禎子、月村亘、伊藤典彦、田島一樹、高橋広樹、関宏也
    • Organizer
      第35回日本バイオマテリアル学会大会
    • Place of Presentation
      タワーホール船堀(東京都)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 再生から創成2013

    • Author(s)
      伊藤典彦、籍禎子
    • Organizer
      鶴田フォーラム
    • Place of Presentation
      東工大蔵前会館(東京都)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 原子間力顕微鏡を用いた3次元培養皮膚モデルの評価方法の検討2013

    • Author(s)
      関禎子、竹内千晶、松田紗苗、皮間未来、月村亘、関宏也、久間將義、伊藤典彦
    • Organizer
      日本実験動物代替法学会
    • Place of Presentation
      京都テルサ(京都市)
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] 生体に対する負荷を評価する方法2011

    • Inventor(s)
      伊藤典彦
    • Industrial Property Rights Holder
      伊藤典彦
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2011-197442
    • Filing Date
      2011-09-09
    • Related Report
      2013 Final Research Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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