Establishment of serum and feeder-free cultured method in human corneal limbal epithelium
| Project/Area Number |
23592618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Ophthalmology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YOKOO Seiichi 東京大学, 医学部附属病院, 特任研究員 (20345052)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 幹細胞疲弊症 / 無血清 / 無フィーダー / 幹細胞疲弊症眼 / 角膜上皮細胞 / 培養法 / KGF / 角膜輪部上皮細胞 / 幹細胞 / 無血清培養 |
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| Outline of Final Research Achievements |
Cultured human corneal epithelial sheet transplantation enable us to treat corneal stem cell deficiency, but this culture technique required bovine serum and mouse 3T3 feeder cells for successful culture. We established culture technique of human corneal limbal sheet with basal medium, B-27 supplement and KGF and are performing clinical study with this undifferentiated culture sheet.
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Report
(5 results)
Research Products
(2 results)
