Establishment of serum and feeder-free cultured method in human corneal limbal epithelium

Research Project

Project/Area Number	23592618
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Multi-year Fund
Section	一般
Research Field	Ophthalmology
Research Institution	The University of Tokyo
Principal Investigator	YAMAGAMI SATORU 東京大学, 医学部附属病院, 准教授 (10220245)
Co-Investigator(Renkei-kenkyūsha)	YOKOO Seiichi 東京大学, 医学部附属病院, 特任研究員 (20345052)
Project Period (FY)	2011-04-28 – 2015-03-31
Project Status	Completed (Fiscal Year 2014)
Budget Amount *help	¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000) Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords	幹細胞疲弊症 / 無血清 / 無フィーダー / 幹細胞疲弊症眼 / 角膜上皮細胞 / 培養法 / KGF / 角膜輪部上皮細胞 / 幹細胞 / 無血清培養
Outline of Final Research Achievements	Cultured human corneal epithelial sheet transplantation enable us to treat corneal stem cell deficiency, but this culture technique required bovine serum and mouse 3T3 feeder cells for successful culture. We established culture technique of human corneal limbal sheet with basal medium, B-27 supplement and KGF and are performing clinical study with this undifferentiated culture sheet.