Hedgehog signal inhibitors suppress the invasion of human rhabdomyosarcoma cells
| Project/Area Number |
23592629
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
OUE Takaharu 大阪大学, 医学(系)研究科(研究院), 講師 (50314315)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZAWA Masahiro 大阪大学, 医学部, 名誉教授 (60165272)
UEHARA Shuichiro 大阪大学, 医学部, 助教 (00448060)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | ヘッジホグ / Gli-1 / 阻害剤 / cyclopamine / forskolin / 細胞増殖 / 横紋筋肉腫 / 分子標的治療 / ヘッジホッグシグナル / 阻害 / Cyclopamine / Forskolin / ヘッジホグシグナル / Cyclipamine |
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| Research Abstract |
The objective of this study was to determine whether Hedgehog signaling plays a role in the invasion of RMS. Two kinds of specific Hh signaling inhibitors, cyclopamine and forskolin, were used to suppress activated Hh signals in three RMS cell lines. The effects of the Hh signaling inhibitors on tumor cell invasion and motility were investigated using Matrigel Invasion assays and wound closure assays, respectively. The number of invaded cells in the Matrigel chambers was significantly decreased. The wound closure assays revealed that a blockade of the Hh signaling pathway impairs RMS cell motility. Both the invasive capacity and motility of RMS cells are significantly suppressed by Hh signaling inhibitors, demonstrating that the Hh pathway plays an important role in the invasion of RMS. Hh inhibitors may provide a new paradigm for the treatment of RMS.
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Report
(4 results)
Research Products
(44 results)
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[Journal Article] Low serum concentrations of anti-Mullerian hormone are common in 53 female childhood cancer survivors2014
Author(s)
Miyoshi Y, Ohta H, Namba N, Tachibana M, Miyamura T, Miyashita E, Hashii Y, Oue T, Isobe A, Tsutsui T, Kimura T, Ozono K
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Journal Title
Hormone Research in Paediatrics
Volume: 79
Issue: 1
Pages: 17-21
DOI
Related Report
Peer Reviewed
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[Journal Article] Low serum concentrations of anti-Mullerian hormone are common in 53 female childhood cancer survivors2013
Author(s)
Miyoshi Y, Ohta H, Namba N, Tachibana M, Miyamura T, Miyashita E, Hashii Y, Oue T, Isobe A, Tsutsui T, Kimura T, Ozono K
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Journal Title
Horm Res Paediatr
Volume: 79
Pages: 17-21
Related Report
Peer Reviewed
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[Journal Article] Methylation of the RASSF1A promoter is predictive of poor outcome among patients with Wilms tumor2012
Author(s)
Ohshima J, Haruta M, Fujiwara W, Watanabe N, Arai Y, Ariga T, Okita H, Koshinaga T, Oue T, Hinotsu S, Nakadate H, Horie H, Fukuzawa M, Kaneko Y
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Journal Title
Pediatr Blood Cancer
Volume: 59
Issue: 3
Pages: 499-505
DOI
Related Report
Peer Reviewed
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[Presentation] Bilateral Wilms'tumors treated according to the Japan Wilms Tumor Study Group protocol2013
Author(s)
Oue T, Fukuzawa M, Okita H, HorieH, Saito M, Nozaki M, Chin M, Nakadate N, Hinotsu S, Koshinaga T, Kaneko Y, Tanaka Y
Organizer
45^<th> Congress of the International Society of Paediatric Oncology
Place of Presentation
Hong Kong, China
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