| Project/Area Number |
23592630
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Osaka University |
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Principal Investigator |
SHUICHIRO Uehara 大阪大学, 医学(系)研究科(研究院), 助教 (00448060)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUZAWA Masahiro 大阪大学, 大学院・医学系研究科, 名誉教授 (60165272)
OUE Takaharu 大阪大学, 大学院・医学系研究科, 講師 (50314315)
NOMURA Motonari 大阪大学, 医学部附属病院, 医員 (40546909)
NAKAHATA Kengo 大阪大学, 医学部附属病院, 医員 (50643532)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 横紋筋肉腫 / 癌幹細胞 / 無血清培地 / ALDH1 / 小児がん / 免疫療法 |
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| Research Abstract |
The objective of this study was to determine the role of cancer stem cell(CSC) in rhabdomyosarcoma. CSC of alveolar rhabdomyosaroma was induced under serum-free medium. These cells exhibited the increase of the abilities of sphere formation, colonization, and tumor formation.
In embryonal type rhabdomyosarcoma cell(RD), we developed the hypothesis that the cells with high ALDH1 activity had the character of the cancer stem cell. The cells with high ALDH1 activity showed the increase of the abilities of sphere formation, colonization, drug resistance and tumor formation. These results suggest that ALDH1 is a relevant marker for alveolar type rhabdomyosarcoma cancer stem cell. Our conclusion is that ALDH1 may be a good candidate for the treatment of RMS.
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