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The development of 3D cartilage regeneration therapy using the human auricular cartilage progenitor cells with autologous serum.

Research Project

Project/Area Number 23592650
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plastic surgery
Research InstitutionYokohama City University

Principal Investigator

KOBAYASHI Shinji  横浜市立大学, 医学(系)研究科(研究院), 客員准教授 (90464536)

Co-Investigator(Kenkyū-buntansha) MAEGAWA Jirou  横浜市立大学, 医学研究科, 教授 (70244449)
TANIGUCHI Hideki  横浜市立大学, 医学研究科, 教授 (70292555)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords軟骨前駆細胞 / 軟骨再生 / 軟骨膜細胞 / 軟骨膜 / 軟骨幹/前駆細胞
Research Abstract

We have succeeded in isolating Human cartilage progenitor cells (hCPCs) and regenerating cartilage derived from them. The hCPCs which were isolated from auricular perichondrium had not only high proliferative and multipotent capacity that human chondrocytes (hCs) did not have, but had same cartilage extracellular matrix capacity as hCs.
Our purpose is to develop handing skill that could apply hCPCs in clinical setting on the basis of their culture technique. Specifically, there are (1) Implementation of hCPCs characterization (2) Evaluation of regenerated cartilage in vivo (3) The development of 3D cartilage regeneration derived from hCPCs (4) The development of autologous serum technique.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (30 results)

All 2014 2012 2011 Other

All Journal Article (13 results) (of which Peer Reviewed: 12 results,  Open Access: 1 results) Presentation (16 results) (of which Invited: 2 results) Book (1 results)

  • [Journal Article] Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes2014

    • Author(s)
      M Mizuno, T Takebe, S Kobayashi, Shintarou Kimura, M Masutani, S Lee, YH Jo, J Ik Lee, H Taniguchi
    • Journal Title

      Transplant Proc

      Volume: 46(4) Pages: 1217-21

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of joint hyaline cartilage by autologous progenitor cells derived from ear elastic cartilage2014

    • Author(s)
      M Mizuno, S Kobayashi, T Takebe, H Kan, Y Yabuki, T Matsuzaki, H Y. Yoshikawa, S Nakabayashi, J,I Lee, J Maegawa, H Taniguchi
    • Journal Title

      Stem Cells

      Volume: 32(3) Pages: 816-21

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of Joint Hyaline Cartilage by Autologous Progenitor Cell s Derived from Ear Elastic Cartilage2014

    • Author(s)
      M. Mizuno, S. Kobayashi, T. Takebe, H. Kan, Y. Yabuki, T. Matsuzaki, H. Y. Yoshikawa, S. Nakabayashi, L. J. Ik, J. Maegawa, H. Taniguchi
    • Journal Title

      Stem Cells

      Volume: 32 Issue: 3 Pages: 816-821

    • DOI

      10.1002/stem.1529

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Elastic cartilage reconstruction by transplantation of cultured hyaline cartilage-derived chondrocytes.2014

    • Author(s)
      Mitsuru Mizuno, Takanori Takebe, Shinji Kobayashi, Shintarou Kimura, Mamiko Masutani, Soojung Lee, Yong Hwa Jo, Jeong Ik Lee, Hideki Taniguchi
    • Journal Title

      Transplant Proc,

      Volume: 46 Issue: 4 Pages: 1217-1221

    • DOI

      10.1016/j.transproceed.2013.12.006

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor2012

    • Author(s)
      T Takebe, S Kobayashi H Kan, Hiromu Suzuki, M Mizuno, Y Yabuki, TAdegawa, T Yoshioka , J Tanaka, J Maegawa, H Taniguchi
    • Journal Title

      Transplantation Proceedings

      Volume: 44(4) Pages: 1158-61

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Human elastic cartilage engineering from cartilage progenitor cells using rotating wall vessel bioreactor.2012

    • Author(s)
      Takanori Takebe, Shinji Kobayashi, Hiroomi Kan, Hiromu Suzuki, Mitsuru Mizuno, Yuichiro Yabuki, Takuro Adegawa, Tomohiko Yoshioka , Junzo Tanaka, Jiro Maegawa, Hideki Taniguchi.
    • Journal Title

      Transplantation Proceedings

      Volume: 44 Issue: 4 Pages: 1158-61

    • DOI

      10.1016/j.transproceed.2012.03.038

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Functional reconstruction of epignathus with cleft palate using part of a mature teratoma.2012

    • Author(s)
      Shinji Kobayashi,, Mari Tanaka, Yukie Ohashi,, Yukichi Tanaka, Jiro Maegawa.
    • Journal Title

      The Cleft Palate-Craniofacial J.

      Volume: 49 Issue: 6 Pages: 69-74

    • DOI

      10.1597/10-246

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium2011

    • Author(s)
      S Kobayashi,, T Takebe, M Mizuno, J Maegawa, H Taniguchi
    • Journal Title

      PLoS ONE

      Volume: 6(10)

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Reconstruction of human elastic cartilage by a CD44^+CD90^+stem cell in the ear perichondrium2011

    • Author(s)
      S Kobayashi, T Takebe, M Inui, S Iwai, H Kan, Y Zheng, J Maegawa, H Taniguchi
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108(35) Pages: 14479-14484

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 軟骨再生医療:こどもの心をいやす医療に向けて最新のコラーゲンサイエンス-生物多様性が促進するバイオ新素材の変革-BIO INDUSTRY2011

    • Author(s)
      小林眞司、谷口英樹
    • Journal Title

      シーエムシー出版

      Volume: BIO INDUSTRY Vol 18 No11 Pages: 32-37

    • Related Report
      2013 Final Research Report
  • [Journal Article] Reconstruction of human elastic cartilage by a CD44+CD90+stem cell in the ear perichondrium.2011

    • Author(s)
      Shinji Kobayashi, Takanori Takebe, Midori Inui, Sayaka Iwai, Hiroomi Kan, Yun-wen Zheng, Jiro Maegawa, Hideki Taniguchi
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108(35) Pages: 14479-14484

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Presence of cartilage stem/progenitor cells in adult mice auricular perichondrium2011

    • Author(s)
      Shinji Kobayashi, Takanori Takebe, Mitsuru Mizuno, Jiro Maegawa, Hideki Taniguchi
    • Journal Title

      PLoS ONE

      Volume: 6(10)

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Ear elevation using two-tiered costal cartilage on the same side as the reconstructed2011

    • Author(s)
      Shinji Kobayashi, J Maegawa
    • Journal Title

      J Craniofac Surg

      Volume: 22(5) Pages: 1796-9

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒト耳介軟骨幹/前駆細胞における自家血清培養法の検証2012

    • Author(s)
      矢吹雄一郎、小林眞司、水野満、廣冨浩一、安村和則、武部貴則、前川二郎、谷口英樹
    • Organizer
      第21回形成外科学会基礎学術集会
    • Place of Presentation
      福島
    • Related Report
      2013 Final Research Report
  • [Presentation] ヒト耳介軟骨膜由来幹/前駆細胞を用いた弾性軟骨再構築法の開発2012

    • Author(s)
      武部貴則、小林眞司、矢吹雄一郎、鈴木啓、水野満、安村和正、広富浩一、鄭允文、前川二郎、谷口英樹
    • Organizer
      第11回日本再生医療学会
    • Place of Presentation
      横浜
    • Related Report
      2013 Final Research Report
  • [Presentation] Reconstruction of human elastic cartilage by progenitor cells in the ear perichondrium2012

    • Author(s)
      Suzuki H, Kobayashi S, Takebe T, Mizuno M, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
    • Organizer
      International Society for Stem Cell Research 10^<th> annual meeting
    • Place of Presentation
      Yokohama,Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells2012

    • Author(s)
      Mizuno M, Kobayashi S Takebe T, Suzuki H, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
    • Organizer
      International Society for Stem Cell Research 10^<th> annual meeting
    • Place of Presentation
      Yokohama,Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Reconstruction of human elastic cartilage by progenitor cells in the ear perichondrium.2012

    • Author(s)
      Suzuki H, Kobayashi S, Takebe T, Mizuno M, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
    • Organizer
      International Society for Stem Cell Research 10th annual meeting.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Reconstitution of Articular(joint) cartilage defects by auricle(ear) derived stem/ progenitor cells.2012

    • Author(s)
      Mizuno M, Kobayashi S, Takebe T, Suzuki H, Murata S, Yabuki Y, Hirotomi K, Yasumura K, Maegawa J, Taniguchi H
    • Organizer
      International Society for Stem Cell Research 10th annual meeting.
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2012 Research-status Report
  • [Presentation] パネルディスカッション歯肉骨膜形成術(GPP)と多血小板血漿(PRP)による乳幼児期での顎裂部骨形成2012

    • Author(s)
      小林眞司、平川崇、府川俊彦、杉本孝之、菅原順、前川二郎
    • Organizer
      第36回日本口蓋裂学会総会・学術集会
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト耳介軟骨幹/前駆細胞における自家血清培養法の検証2012

    • Author(s)
      矢吹雄一郎、小林 眞司、水野 満、廣冨 浩一、安村 和則、武部 貴則、前川 二郎、谷口 英樹
    • Organizer
      第21回形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代(福島県)
    • Related Report
      2012 Research-status Report
  • [Presentation] 乳児期の真皮中には多分化能を持つ細胞が存在する2012

    • Author(s)
      小林眞司、水野満、村田駿介、武部貴則、鈴木啓、 矢吹雄一郎、 安村和則、 前川二郎、 谷口英樹
    • Organizer
      第21回形成外科学会基礎学術集会
    • Place of Presentation
      ホテルリステル猪苗代(福島県)
    • Related Report
      2012 Research-status Report
  • [Presentation] ヒト弾性軟骨膜細胞由来の再生軟骨を構成する軟骨膜の特性解析2011

    • Author(s)
      小林眞司、武部貴則、水野満、鈴木啓、矢吹雄一郎、鄭允文、前川二郎、谷口英樹
    • Organizer
      第20回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification and utilization of human cartilage stem cells from the ear2011

    • Author(s)
      Suzuki H, Kobayashi S, Takebe T, Mizuno M, Yabuki Y, Yasumura K, Murata S, Maegawa J, Taniguchi H
    • Organizer
      The 12th Congress of the Asian Society of Transplantation
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2013 Final Research Report
  • [Presentation] イヌ耳介由来軟骨幹/前駆細胞を用いた関節軟骨再生療法の開発2011

    • Author(s)
      水野満、小林眞司、武部貴則、鈴木啓、村田駿介、矢吹雄一郎、安村和則、前川二郎、谷口英樹
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪
    • Related Report
      2013 Final Research Report
  • [Presentation] Identification and utilization of human cartilage stem cells from the ear2011

    • Author(s)
      Hiromu Suzuki, Shinji Kobayashi, Takanori Takebe, Mitsuru Mizuno,Yuichiro Yabuki, Kazumusa Yasumura, Syunsuke Murata,Jiro Maegawa, Hideki Taniguchi
    • Organizer
      12th Congress of the Asian Society of Transplantation,
    • Place of Presentation
      Seoul, Korea
    • Related Report
      2011 Research-status Report
  • [Presentation] イヌ耳介由来軟骨幹/前駆細胞を用いた関節軟骨再生療法の開発2011

    • Author(s)
      水野満、小林眞司、武部貴則、鈴木啓、村田駿介、矢吹雄一郎、安村和則、前川二郎、谷口英樹
    • Organizer
      第152回日本獣医学会学術集会
    • Place of Presentation
      大阪府立大学(大阪府)
    • Related Report
      2011 Research-status Report
  • [Presentation] 軟骨間葉系幹/前駆細胞を用いたこれからの軟骨再生療法

    • Author(s)
      小林眞司
    • Organizer
      第47回日本形成外科学会中部支部学術集会
    • Place of Presentation
      信州大学(長野県)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 口唇口蓋裂に対する再生医学的アプローチ ~乳児期での多血小板血漿(PRP)による骨再生~

    • Author(s)
      小林眞司
    • Organizer
      第2回細胞再生医療研究会
    • Place of Presentation
      臨床研究情報センター(兵庫県)
    • Related Report
      2012 Research-status Report
    • Invited
  • [Book] シーエムシー出版2011

    • Author(s)
      小林眞司、谷口英樹
    • Total Pages
      6
    • Publisher
      軟骨再生医療:こどもの心をいやす医療に向けて
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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