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Effect of Adipose-Derived Stem Cells and Cross-linking of Collagen on Acellular Dermal Matrix

Research Project

Project/Area Number 23592658
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Plastic surgery
Research InstitutionNippon Medical School

Principal Investigator

TAKAMI Yoshihiro  日本医科大学, 医学部, その他 (30201601)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords無細胞真皮 / 結合組織 / 再生医学 / 幹細胞 / 脂肪組織
Research Abstract

It has been known that an acellular dermal matrix (ADM) is partially resorbed after transplanting. In this study, the effectiveness of adipose-derived stem cells (ASCs) and collagen cross-linking for the resorption of ADM was investigated. ADM was prepared from the skin of Fischer rats with trypsin. Cross-linking of ADM was performed with glutaraldehyde. ASCs from the inguinal fat pads were cultivated and the cells were seeded on ADMs. ADM with ASCs, cross-linked ADM and untreated ADM were respectively transplanted subcutaneously or into the defects of Achilles tendon of rats. As compared to the untreated ADMs, the subcutaneously transplanted ADM with ASCs significantly inhibited the ADM resorption. Although the cross-linked ADM inhibited the resorption, neo-vascularization was less induced than the ADM with ASCs. In the tendon regeneration, the ADMs with ASCs showed the better tendinous regeneration than the other types of ADMs. ASCs appeared effective for inhibiting ADM resorption.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (56 results)

All 2014 2013 2012 2011 Other

All Journal Article (18 results) (of which Peer Reviewed: 18 results) Presentation (31 results) (of which Invited: 1 results) Book (5 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix2014

    • Author(s)
      Yoshihiro Takami, Ryo Yamaguchi, Shimpei Ono, Hiko Hyakusoku
    • Journal Title

      J Nippon Med Sch

      Volume: 81in press

    • NAID

      130004926720

    • URL

      http://www.nms.ac.jp/jnms/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Clinical application and histological properties of autologous tissue-engineered skin equivalents using an acellular dermal matrix2014

    • Author(s)
      Yoshihiro Takami, et al.
    • Journal Title

      J Nippon Med Sch.

      Volume: 81

    • NAID

      130004926720

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Functional damage of human dermal lymphatic endothelial cells by a high glucose condition.2014

    • Author(s)
      Matsumoto N, Takami Y, Ono S, Sugimoto A, Hyakusoku H
    • Journal Title

      Wound Repair and Regeneration

      Volume: 22 Issue: 3 Pages: 415-423

    • DOI

      10.1111/wrr.12159

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 重症感染性糖尿病性足壊疽に対する治療戦略:治療のプロトコール化について2013

    • Author(s)
      高見佳宏、秋山豪、長嶋有紀、櫻井透、飯村剛史、百束比古
    • Journal Title

      Skin Surgery

      Volume: 22 Pages: 159-162

    • NAID

      40022698051

    • URL

      http://www.jsds.jp/.

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Feasibility of dermis grafts in burn surgery2013

    • Author(s)
      Takami Y, Hyakusoku H
    • Journal Title

      Burns

      Volume: 39 Pages: 369-370

    • URL

      http://www.journals.elsevier.com/burns/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] 外来の小外科―その標準化に向けて2013

    • Author(s)
      高見佳宏、百束比古
    • Journal Title

      日本医師会雑誌

      Volume: 141 (10) Pages: 2269-2269

    • URL

      http://www.med.or.jp/cme/jjma/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Takami Y, Hyakusoku H2013

    • Author(s)
      Takami Y, Hyakusoku H
    • Journal Title

      Burns

      Volume: 39 Pages: 369-370

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 重症感染性糖尿病性足壊疽に対する治療戦略:治療のプロトコール化について2013

    • Author(s)
      高見佳宏、秋山豪、長嶋有紀、櫻井透、飯村剛史、百束比古
    • Journal Title

      Skin Surgery

      Volume: 22 Pages: 159-162

    • NAID

      40022698051

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effect of adipose-derived stenm cells on tendon regeneration using acellular dermal matrix2013

    • Author(s)
      Yoshihiro Takami, et. al.
    • Journal Title

      Wound Repair and Regeneration

      Volume: 21

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Feasibility of dermis grafts in burn surgery.2013

    • Author(s)
      Takami Y, Hyakusoku H.
    • Journal Title

      Burns

      Volume: 39 Pages: 369-372

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] How large can a pedicled flap be?2012

    • Author(s)
      Ono S, Ogawa R, Hayashi H, Takami,Y, Hyakusoku H
    • Journal Title

      Plast Reconstr Surg

      Volume: 130(1)

    • URL

      http://journals.lww.com/plasreconsurg/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perforator-supercharged perforator-based propeller flaps2012

    • Author(s)
      Ono S, Ogawa R, Eura S, Takami Y, Hyakusoku H
    • Journal Title

      Plast Reconstr Surg

      Volume: 129(5)

    • URL

      http://journals.lww.com/plasreconsurg/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perforator -supercharged Occipito -cervico-pectoral (OCP)Flaps for Lower Face and Neck Reconstruction2012

    • Author(s)
      Ono S, Chung KC, Takami Y, Chin T,Ogawa R, Gao JH, Hyakusoku H
    • Journal Title

      Plast Reconstr Surg

      Volume: 129(4) Pages: 879-887

    • URL

      http://journals.lww.com/plasreconsurg/

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Perforator-supercharged local perforator -based (LP) flaps.2012

    • Author(s)
      Ono S , Ogawa R, Takami Y, Hyakusoku H.
    • Journal Title

      J Plast Reconstr Aesthetic Surg.

      Volume: 65 Pages: 834-836

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Perforator-supercharged perforator-based propeller flaps.2012

    • Author(s)
      Ono S, Ogawa R, Eura S, Takami Y, Hyakusoku H.
    • Journal Title

      Plast Reconstr Surg.

      Volume: 129

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Perforator-supercharged Occipito-cervico-pectoral (OCP) Flaps for Lower Face and2012

    • Author(s)
      Ono S, Chung KC, Takami Y, Chin T, Ogawa R, Gao JH, Hyakusoku H
    • Journal Title

      Plast Reconstr Surg

      Volume: 129 Pages: 879-887

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Influence of negative atmospheric pressure on cultured human dermal fibroblasts2012

    • Author(s)
      Yoshihiro Takami, Jingping Guo, Shimpei Ono, Hakan Orbay, Rei Ogawa, Hiko Hyakusoku
    • Journal Title

      Journal of Wound Technology,

      Volume: 15 Pages: 62-62

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Acellular Dermal Matrix Seeded with Adipose-Derived Stem Cells as a Subcutaneous Implant2011

    • Author(s)
      Orbay H, Takami Y, Hyakusoku H, Mizuno H
    • Journal Title

      Aesthetic Plast Surg

      Volume: 35(5) Issue: 5 Pages: 756-763

    • DOI

      10.1007/s00266-011-9683-2

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Presentation] 無細胞真皮マトリックスによる腱再生:脂肪組織由来幹細胞とコラーゲン架橋による影響について2013

    • Author(s)
      高見佳宏、小野真平、百束比古
    • Organizer
      第43回日本創傷治癒学会
    • Place of Presentation
      別府
    • Year and Date
      2013-11-15
    • Related Report
      2013 Final Research Report
  • [Presentation] 重症感染性足趾壊疽の治療指針:救肢までの段階的なプロトコルについて2013

    • Author(s)
      高見佳宏、秋山豪、長嶋有紀、櫻井透、百束比古
    • Organizer
      第55回全日本病院学会
    • Place of Presentation
      大宮
    • Year and Date
      2013-11-03
    • Related Report
      2013 Final Research Report
  • [Presentation] アルデヒド処理ラット血管を用いた微小血管吻合のトレーニング2013

    • Author(s)
      高見佳宏、大澤幸代、中馬久美子、石井啓子、百束比古
    • Organizer
      第81回日本医科大学医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2013-09-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 皮膚外科手術2013

    • Author(s)
      高見佳宏
    • Organizer
      2013年度日本臨床皮膚外科学会,教育セミナー
    • Place of Presentation
      東京
    • Year and Date
      2013-07-07
    • Related Report
      2013 Final Research Report
  • [Presentation] 大切断を回避する重症感染性糖尿病性壊疽の治療戦略2013

    • Author(s)
      高見佳宏、長嶋有紀、秋山豪、飯村剛史、百束比古
    • Organizer
      第31回日本臨床皮膚外科学会学術大会
    • Place of Presentation
      高雄、台湾
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂肪由来幹細胞とコラーゲン架橋による無細胞真皮マトリックスの移植特性の変化2012

    • Author(s)
      高見佳宏、Hakan Orbay、小野真平、百束比古
    • Organizer
      第42回日本創傷治癒学会
    • Place of Presentation
      札幌
    • Year and Date
      2012-12-04
    • Related Report
      2013 Final Research Report
  • [Presentation] 高濃度グルコース環境による皮膚リンパ管内皮細胞障害2012

    • Author(s)
      松本典子、高見佳宏、杉本貴子、小野真平、百束比古
    • Organizer
      第42回日本創傷治癒学会
    • Place of Presentation
      札幌
    • Year and Date
      2012-12-03
    • Related Report
      2013 Final Research Report
  • [Presentation] 高濃度グルコースによる培養皮膚リンパ管内皮細胞の変化2012

    • Author(s)
      高見佳宏、百束比古、他
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      福島
    • Year and Date
      2012-10-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Tendon regeneration with acellular dermal matrix and adipose-derived stem cells2012

    • Author(s)
      Yoshihiro Takami, Shimpei Ono, Hakan Orbay, Goh Akiyama, Yuki Nagashima, Teruyuki Dohi, Hiko Hyakusoku
    • Organizer
      4^<th> Congress of the world union of wound healing societies
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2012-09-05
    • Related Report
      2013 Final Research Report
  • [Presentation] 高濃度グルコースによる培養皮膚リンパ管内皮細胞の変化2012

    • Author(s)
      高見佳宏、松本典子、百束比古
    • Organizer
      第80回日本医科大学医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-01
    • Related Report
      2013 Final Research Report
  • [Presentation] ラット腹部皮弁におけるSuperchargeの効果について2012

    • Author(s)
      松本典子、高見佳宏、百束比古
    • Organizer
      第80回日本医科大学医学会総会
    • Place of Presentation
      東京
    • Year and Date
      2012-09-01
    • Related Report
      2013 Final Research Report
  • [Presentation] Conservative therapy using the artificial dermis for intractable ulcer of the skin2012

    • Author(s)
      Yuki Nagashima, Goh Akiyama, Teruyuki Dohi, Yoshihiro Takami, Hiko Hyakusoku
    • Organizer
      4^<th> Congress of the world union of wound healing societies
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] 脂肪組織由来幹細胞による無細胞真皮マトリックスの移植特性影響2012

    • Author(s)
      高見佳宏、Hakan Orbay、秋山豪、他
    • Organizer
      第30回日本臨床皮膚外科学会学術大会
    • Place of Presentation
      ハノイ、ベトナム
    • Related Report
      2013 Final Research Report
  • [Presentation] 皮膚難治性潰瘍に対する人工真皮を用いた保存的治療.2012

    • Author(s)
      高見佳宏、長嶋有紀、秋山豪ほか
    • Organizer
      第30回日本臨床皮膚外科学会学術大会
    • Place of Presentation
      ハノイ、ベトナム
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂肪組織由来幹細胞による無細胞真皮マトリックスの移植特性への影響.2012

    • Author(s)
      高見佳宏、Hakan Orbay、秋山豪ほか
    • Organizer
      第30回日本臨床皮膚外科学会学術大会
    • Place of Presentation
      ハノイ、ベトナム
    • Related Report
      2011 Research-status Report
  • [Presentation] 創傷治癒の基礎とアップデート2011

    • Author(s)
      高見佳宏
    • Organizer
      日本臨床皮膚外科学会,教育セミナー
    • Place of Presentation
      東京
    • Year and Date
      2011-07-04
    • Related Report
      2013 Final Research Report
  • [Presentation] Tissue regeneration using acellular dermal matrix2011

    • Author(s)
      Yoshihiro Takami
    • Organizer
      1^<st> Sino-European Joint meeting of Wound Healing
    • Place of Presentation
      Shanghai, China
    • Related Report
      2013 Final Research Report
  • [Presentation] 組織再生のための新知見2:脂肪組織幹細胞と無細胞真皮マトリックスによる腱組織再生2011

    • Author(s)
      高見佳宏、Hakan Orbay、他
    • Organizer
      第41回日本創傷治癒学会
    • Place of Presentation
      名古屋
    • Related Report
      2013 Final Research Report
  • [Presentation] 幹細胞と無細胞真皮マトリックスによる腱再生2011

    • Author(s)
      高見佳宏、Hakan Orbay、秋山豪、他
    • Organizer
      第20回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京
    • Related Report
      2013 Final Research Report
  • [Presentation] Tissue regeneration using acellular dermal matrix.2011

    • Author(s)
      Yoshihiro Takami
    • Organizer
      1st Sino-European Joint meeting of Wound Healing(招待講演)
    • Place of Presentation
      Shanghai, China.
    • Related Report
      2011 Research-status Report
  • [Presentation] 幹細胞と無細胞真皮マトリックスによる腱再生.2011

    • Author(s)
      高見佳宏、Hakan Orbay、秋山豪ほか
    • Organizer
      第20回日本形成外科学会基礎学術集会
    • Place of Presentation
      東京
    • Related Report
      2011 Research-status Report
  • [Presentation] 脂肪組織幹細胞と無細胞真皮マトリックスによる腱組織再生.2011

    • Author(s)
      高見佳宏、Hakan Orbay、秋山豪ほか
    • Organizer
      第41回日本創傷治癒学会
    • Place of Presentation
      名古屋
    • Related Report
      2011 Research-status Report
  • [Presentation] 無細胞真皮マトリックスによる腱再生:脂肪組織由来幹細胞とコラーゲン架橋による影響について

    • Author(s)
      高見佳宏、小野真平、百束比古
    • Organizer
      第43回 日本創傷治癒学会
    • Place of Presentation
      別府、大分
    • Related Report
      2013 Annual Research Report
  • [Presentation] アルデヒド処理ラット血管を用いた微小血管吻合のトレーニング

    • Author(s)
      高見佳宏、ほか.
    • Organizer
      第81回日本医科大学医学会総会
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
  • [Presentation] 重症感染性足趾壊疽の治療指針:救肢までの段階的なプロトコルについて.

    • Author(s)
      高見佳宏、ほか.
    • Organizer
      第55回全日本病院学会
    • Place of Presentation
      さいたま市
    • Related Report
      2013 Annual Research Report
  • [Presentation] 皮膚外科手術

    • Author(s)
      高見佳宏
    • Organizer
      日本臨床皮膚外科学会、教育セミナー 2013
    • Place of Presentation
      東京
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Tendon regeneration with acellular dermal matrix and adipose-derived stem cells.

    • Author(s)
      Yoshihiro Takami, et.al.
    • Organizer
      4th Congress of the world union of wound healing societies
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] Conservative therapy using the artificial dermis for intractable ulcer of the skin.

    • Author(s)
      Yuki Nagashima, Yoshihiro Takami, et.al.
    • Organizer
      4th Congress of the world union of wound healing societies
    • Place of Presentation
      Tokyo, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] 脂肪由来幹細胞とコラーゲン架橋による無細胞真皮マトリックスの移植特性の変化.

    • Author(s)
      高見佳宏、ほか.
    • Organizer
      第42回日本創傷治癒学会
    • Place of Presentation
      札幌
    • Related Report
      2012 Research-status Report
  • [Presentation] 高濃度グルコースによる培養皮膚リンパ管内皮細胞の変化

    • Author(s)
      高見佳宏、ほか.
    • Organizer
      第21回日本形成外科学会基礎学術集会
    • Place of Presentation
      福島
    • Related Report
      2012 Research-status Report
  • [Presentation] 高濃度グルコース環境による培養皮膚リンパ管内皮細胞の変化.

    • Author(s)
      高見佳宏、ほか
    • Organizer
      第80回 日本医科大学医学会総会
    • Place of Presentation
      東京
    • Related Report
      2012 Research-status Report
  • [Book] 形成外科手術手技全書2014

    • Author(s)
      高見佳宏
    • Publisher
      克誠堂
    • Related Report
      2013 Annual Research Report
  • [Book] 熱傷・熱傷潰瘍.傷のきれいな治し方.第2版、(百束比古、小川令編集)2012

    • Author(s)
      高見佳宏
    • Publisher
      東京、全日本病院出版会
    • Related Report
      2013 Final Research Report
  • [Book] 傷のきれいな治し方. 第2版、百束比古、小川令 編集:熱傷・熱傷潰瘍.2012

    • Author(s)
      高見佳宏
    • Total Pages
      191
    • Publisher
      全日本病院出版会
    • Related Report
      2012 Research-status Report
  • [Book] 熱傷の治療.アトラス形成外科手術手技.(百束比古編集)2011

    • Author(s)
      高見佳宏
    • Publisher
      東京、中外医学社
    • Related Report
      2013 Final Research Report
  • [Book] 手術手技アトラス「無細胞化組織」

    • Author(s)
      高見佳宏
    • Publisher
      東京、克誠堂出版2014出版予定
    • Related Report
      2013 Final Research Report
  • [Patent(Industrial Property Rights)] 手袋用粘着性低減組成物2013

    • Inventor(s)
      高見佳宏
    • Industrial Property Rights Holder
      日本医科大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-01-30
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 特願2013-154322013

    • Inventor(s)
      高見佳宏
    • Industrial Property Rights Holder
      高見佳宏
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-01-30
    • Related Report
      2012 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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