| Project/Area Number |
23592659
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Plastic surgery
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INOUE Hajime 聖マリアンナ医科大学, 医学部, 教授 (60193603)
TOMIOKA Miyuki 聖マリアンナ医科大学, 医学部, 研究技術員 (90398967)
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| Project Period (FY) |
2011-04-28 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 高ブドウ糖 / 低ブドウ糖 / 表皮細胞 / Sortilin-1 / 糖尿病性神経障害 / Nerve growth factor / Matrix Metalloprotease-9 / 線維芽細胞 / 糖尿病性皮膚障害 |
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| Outline of Final Research Achievements |
When proNGF binds to sortilin and p75, the cell death signal is transduced into the cell. We examined how sortilin gene expression changes in cultured human epidermal cells depending on the glucose concentration in the culture medium.
When the cells were grown in the new medium for 24 hours, the sortilin gene expression level at 100 mg/dL glucose did not differ significantly from the expression levels at other glucose concentrations. At 48 and 72 hours of culture, only the sortilin gene expression level at 0 mg/dL glucose was significantly higher than the expression level at 100 mg/dL glucose. The results suggest that the sortilin gene levels of epidermal cells are not clinically problematic at low glucose concentrations, just as at high glucose concentrations, provided the glucose levels are sufficient to maintain biological activities.
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