Analysis of nerve cells, keratinocytes and fibroblasts in a high glucose medium

• Project/Area Number: 23592659
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: MATSUZAKI Kyoichi 聖マリアンナ医科大学, 医学部, 准教授 (20278013)
• Co-Investigator(Kenkyū-buntansha): INOUE Hajime 聖マリアンナ医科大学, 医学部, 教授 (60193603) ; TOMIOKA Miyuki 聖マリアンナ医科大学, 医学部, 研究技術員 (90398967)
• Project Period (FY): 2011-04-28 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 高ブドウ糖 / 低ブドウ糖 / 表皮細胞 / Sortilin-1 / 糖尿病性神経障害 / Nerve growth factor / Matrix Metalloprotease-9 / 線維芽細胞 / 糖尿病性皮膚障害

Outline of Final Research Achievements

When proNGF binds to sortilin and p75, the cell death signal is transduced into the cell. We examined how sortilin gene expression changes in cultured human epidermal cells depending on the glucose concentration in the culture medium.
When the cells were grown in the new medium for 24 hours, the sortilin gene expression level at 100 mg/dL glucose did not differ significantly from the expression levels at other glucose concentrations. At 48 and 72 hours of culture, only the sortilin gene expression level at 0 mg/dL glucose was significantly higher than the expression level at 100 mg/dL glucose. The results suggest that the sortilin gene levels of epidermal cells are not clinically problematic at low glucose concentrations, just as at high glucose concentrations, provided the glucose levels are sufficient to maintain biological activities.

Research Products

• [Journal Article] Expression of the Sortilin Gene in Cultured Human Keratinocytes Increases in a Glucose-free Medium (2014)
  • Author(s): Kyoichi Matsuzaki, Miyuki Tomioka, Yuichi Watabe
  • Journal Title: Clinical Research on Foot & Ankle Volume: S3 Issue: 01 Pages: 1-4
  • DOI: 10.4172/2329-910x.s3-004
  • Peer Reviewed / Open Access
• [Presentation] 糖尿病性腎症により血液透析が導入された重症下肢虚血患者の足創傷をカテーテルと小切断で治療後2年の転帰 (2014)
  • Author(s): 松崎恭一、宮本明
  • Organizer: 第57回日本形成外科学会総会・学術集会
  • Place of Presentation: 長崎ブリックホール、長崎新聞文化ホール、NCC & スタジオ（長崎県・長崎市）
  • Year and Date: 2014-04-09 – 2014-04-11