| Project/Area Number |
23592672
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TESHIMA Yasushi 大分大学, 医学部, 助教 (10457608)
HAGIWARA Satoshi 大分大学, 医学部, 講師 (50527661)
SAIKAWA Tetsunori 大分大学, 医学部, 教授 (60145365)
NOGUCHI Takayuki 大分大学, 医学部, 教授 (90156183)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 心房細動 / メタボリック症候群 / 酸化ストレス / 虚血再灌流障害 |
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| Research Abstract |
We examined the hypothesis that leptin signaling contributes to the atrial fibrosis and atrial fibrillation (AF) evoked by angiotensin II (AngII). Male CL57/B6 (CNT) and leptin-deficient ob/ob mice (Ob) were subcutaneously infused with AngII. In CNT-AngII mice, leptin expression in the left atrium was upregulated. Transesophageal burst pacing induced AF in 88% of CNT-AngII mice, but not in Ob-AngII mice (0%). In isolated perfused hearts, AF was induced only in CNT-AngII mice (67%). Inter-atrial conduction time was prolonged in CNT-AngII mice, but not in Ob-AngII mice. In cultured SD rat atrial fibroblasts, AngII treatment increased leptin expression. Addition of leptin increased TGF-beta1, alpha-SMA, MCP-1 and RANTES expressions in SD rat atrial fibroblasts but not in Zucker rat atrial fibroblasts. These observations demonstrate that leptin signaling is essential for the development of atrial fibrosis and AF evoked by AngII.
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