| Project/Area Number |
23592679
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
UEDA Kentaro 和歌山県立医科大学, 医学部, 助教 (20438279)
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| Co-Investigator(Kenkyū-buntansha) |
KIDA Maki 和歌山県立医科大学, 医学部, 助教 (00326381)
YONEMITSU Takafumi 和歌山県立医科大学, 医学部, 助教 (80382331)
中 敏夫 和歌山県立医科大学, 医学部, 准教授 (00244757)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | マウス腸管癒着モデル / siPAI-1 HVJ vector / SOCS1 HVJ vector / HVJ Envelope vector / PAI-1 siRNA / SOCS1 / 術後腸管癒着 / PAI-1 SiRNA |
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| Research Abstract |
We constructed SOCS1 expression vector to suppress STAT1 specifically and PAI-1 siRNA vector, and examined the prevention effect of adhesion in mouse intestinal adhesion model using these vectors.
We had set the 5 group: A: Control group, B: SOCS1 group, C: PAI-1 siRNA group, D: SOCS1 + PAI-1 siRNA group, F: HGF (positive control). The results were equivalent to that of the F group was no difference in the three groups A, B, and C histology with the degree of adhesion, but adhesion is improved edge in the D group and E group. We found no side effects of bone marrow, liver, to the kidney in all groups.
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