| Project/Area Number |
23592691
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Emergency medicine
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| Research Institution | National Cardiovascular Center Research Institute |
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Principal Investigator |
AKIYAMA Tsuyoshi 独立行政法人国立循環器病研究センター, 研究所, 室長 (70202554)
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| Co-Investigator(Renkei-kenkyūsha) |
SONOBE Takashi 独立行政法人国立循環器病研究センター, 研究所, 流動研究員 (70548289)
SHIMIZU Shuji 独立行政法人国立循環器病研究センター, 研究所, 特任研究員 (80443498)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 心臓 / 虚血・再灌流 / セロトニン / 5-HIAA / マイクロダイアリシス法 |
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| Research Abstract |
Using microdialysis technique, we monitored myocardial interstitial 5-HT and its metabolite by monoamine oxidase (MAO), 5-HIAA levels in the ischemic region of anesthetized rat or rabbit during ischemia and reperfusion. In vehicle, 5-HT levels increased during ischemia, but 5-HIAA levels decreased during ischemia. After reperfusion 5-HT levels transiently increased and then declined. 5-HIAA levels increased after reperfusion and kept high level. Fluoxetine decreased 5-HT levels during ischemia, but did not change 5-HIAA levels after reperfusion. Pargyline increased 5-HT levels after reperfusion and decreased 5-HIAA levels after reperfusion. During ischemia, the carrier-mediated 5-HT efflux could contribute to the increase in 5-HT levels and the decrease in 5-HIAA levels could be ascribed to the reduction of MAO activity due to oxygen depletion and acidosis. After reperfusion, washout to blood as well as removal by uptake carrier could contribute to the decrease in 5-HT levels.
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