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Development of a method to remove mycoplasmas from the contaminated cell cultures using antimicrobial photodynamic therapy

Research Project

Project/Area Number 23592692
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionHokkaido University

Principal Investigator

HASEBE Akira  北海道大学, 歯学研究科(研究院), 助教 (90281815)

Co-Investigator(Kenkyū-buntansha) 柴田 健一郎  北海道大学, 大学院歯学研究科, 教授 (50145265)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords光線力学療法 / マイコプラズマ汚染 / 培養細胞 / マイコプラズマ / aPDT / contamination
Research Abstract

Mycoplasmas are one of the most frequent contaminants due to their characteristics in cell cultures. It is required to develop a method to remove mycoplasmas without using antibiotics, because a treatment with antibiotics may induce antibiotic-resistant bacteria. This study attempted to remove cell culture-contaminating Mycoplasma salivarium (Ms) by utilizing antimicrobial photodynamic therapy with a cordless red light emitting diode. Ms-infected human embryonic kidney 293 cells was irradiated with red light emitting diode in the presence of methylene blue as a photosensitizer, after decreasing the number of live Ms by washing with sterilized phosphate-buffered saline. No MS was detected in the contaminated cells by both mycoplasma detection kit and cultivation after antimicrobial photodynamic therapy, demonstrating that antimicrobial photodynamic therapy successfully remove Ms from its contaminated cells without giving damages to the cells.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (4 results)

All 2013 2011 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (1 results) Remarks (1 results)

  • [Journal Article] Mycoplasma removal from cell culture using antimicrobial photodynamic therapy2013

    • Author(s)
      Akira Hasebe, Isao Ishikawa, Haque M. Shamsul, Makoto Ohtani, Taku Segawa, Ayumi Saeki, Naoho Tanizume, Manabu Oouchi, Yoshihide Okagami, Teruo Okano, and Ken-ichiro Shibata
    • Journal Title

      Photomedicine and Laser Surgery

      Volume: Vol. 31, No. 3

    • NAID

      120005372314

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mycoplasma Removal from Cell Culture Using Antimicrobial Photodynamic Therapy2013

    • Author(s)
      Akira Hasebe, Isao Ishikawa, Haque M. Shamsul, Makoto Ohtani, Taku Segawa, Ayumi Saeki, Naoho Tanizume, Manabu Oouchi, Yoshihide Okagami, Teruo Okano, Ken-ichiro Shibata
    • Journal Title

      Photomedicine and Laser Surgery

      Volume: 31 Issue: 3 Pages: 125-131

    • DOI

      10.1089/pho.2012.3372

    • NAID

      120005372314

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Presentation] Roles of the src family kinase lck in Toll-like receptor 2 signaling triggered by stimulation with a diacylated lipopeptide FSL-1.2011

    • Author(s)
      Akira Hasebe, Naoho Tanizume, Makoto Ohtani, Ken-Ichiro Shibata.
    • Organizer
      International Union of Microbiological Societies 2011 Congress
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2011 Research-status Report
  • [Remarks]

    • URL

      http://www.den.hokudai.ac.jp/saikin/achievements/2013.html

    • Related Report
      2013 Final Research Report

URL: 

Published: 2011-08-05   Modified: 2019-07-29  

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