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Investigation of the role of MMP in bone healing with mineral and proteome analyses

Research Project

Project/Area Number 23592694
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionTohoku University

Principal Investigator

SASANO YASUYUKI  東北大学, 歯学研究科(研究院), 教授 (30196191)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Megumi  東北大学, 大学院歯学研究科, 助教 (20431512)
Co-Investigator(Renkei-kenkyūsha) TAKAHASHI Nobuhiro  東北大学, 大学院歯学研究科, 教授 (60183852)
Project Period (FY) 2011-04-28 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords骨 / 修復 / 石灰化 / マイクロCT / 分析走査電子顕微鏡 / 頭蓋骨 / ラット / 発生・成長 / osteocadherin / プロテオグリカン / 発生 / 成長
Outline of Final Research Achievements

The present study was designed to investigate the process of calcification during bone healing in a standardized rat calvarial bone defect model. A standard defect with diameter 3.8 mm was made on the parietal bone of 12-week-old male Wistar rats under anesthesia. The healing bone was analyzed with micro-computed tomography and energy dispersive X-ray spectroscopy in weeks 1,2,4 and 8. The bone mineral density and the Ca/P ratio increased, whereas the C/Ca and C/P ratios decreased during bone healing. The present study demonstrated that the mineral components increase in density and mature in quality while organic components decrease. Our following study revealed the comparable calcification process during development of rat calvarial bone development.

Report

(5 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Research-status Report
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (19 results)

All 2015 2014 2013 2012 2011

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Acknowledgement Compliant: 1 results) Presentation (14 results) (of which Invited: 2 results)

  • [Journal Article] Bone matrix calcification during embryonic and postembryonic rat calvarial development assessed by SEM-EDX, XRD and FTIR.2015

    • Author(s)
      Henmi A, Okata H, Anada T, Yoshinari M, Mikami Y, Suzuki O, Sasano Y
    • Journal Title

      J Bone Miner Metab

      Volume: in press Issue: 1 Pages: 41-50

    • DOI

      10.1007/s00774-014-0647-x

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Calcification during bone healing in a standardised rat calvarial defect assessed by micro- CT and SEM- EDX2014

    • Author(s)
      Okata H, Nakamura M, Henmi A, Yamaguchi S, Mikami Y, Shimauchi H, Sasano Y
    • Journal Title

      Oral Diseases

      Volume: in press Issue: 1 Pages: 74-82

    • DOI

      10.1111/odi.12212

    • Related Report
      2013 Research-status Report
    • Peer Reviewed
  • [Journal Article] Physiological distal drift in rat molars contributes to acellular cementum formation.2013

    • Author(s)
      Tsuchiya S, Tsuchiya M, Nishioka T, Suzuki O, Sasano Y, Igarashi K
    • Journal Title

      Anatomical Record-Advances in Integrative Anatomy and Evolutionary Biology

      Volume: in press

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Involvement of sensory neuron in bone defect repair in rats2011

    • Author(s)
      Henmi A, Nakamura M, 他
    • Journal Title

      J Electron Microsc

      Volume: 60 Issue: 6 Pages: 393-400

    • DOI

      10.1093/jmicro/dfr075

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Journal Article] Characterization of the calcification process modeled in rat embryonic calvarial culture2011

    • Author(s)
      Kimura Y, Kikunaga S, Takahashi I, Hatakeyama Y, Fukumoto S, Sasano Y
    • Journal Title

      J Electron Microsc

      Volume: 60 Issue: 5 Pages: 345-52

    • DOI

      10.1093/jmicro/dfr068

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] Extracellular Matrix Remodeling and Biomineralization in Bone during Development and Healing.2014

    • Author(s)
      Sasano Y(笹野泰之)
    • Organizer
      3rd International Symposium on Dental Implantology and Biomaterials of West Coast Strait
    • Place of Presentation
      Fuzhou, China
    • Year and Date
      2014-11-23
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] ラット切歯エナメル質と象牙質の石灰化過程におけるCa,PおよびCのSEM-EDX分析2014

    • Author(s)
      丸山顕太郎、逸見 晶子、笹野 泰之
    • Organizer
      第56回歯科基礎医学会学術大会
    • Place of Presentation
      福岡市
    • Year and Date
      2014-09-25 – 2014-09-27
    • Related Report
      2014 Annual Research Report
  • [Presentation] Remodeling of Extracellular Matrices and Biomineralization during Bone Development and Healing2014

    • Author(s)
      Sasano Y(笹野泰之)
    • Organizer
      International Symposium on Biomineralization
    • Place of Presentation
      Gwangju, Korea
    • Year and Date
      2014-06-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Calcification in the bone matrix during rat calvarial development2014

    • Author(s)
      Henmi A, Okata H, Anada T, Yoshinari M, Mikami Y, Suzuki O, Sasano Y
    • Organizer
      The 6th International Symposiumfor Interface Oral Health Science
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Calcification in healing calvarial bone assessed by micro- CT and SEM- EDX2014

    • Author(s)
      Okata H, Nakamura M, Henmi A, Yamaguchi S, Sasano Y
    • Organizer
      The 6th International Symposiumfor Interface Oral Health Science
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] Calcification in rat developing mandibular bone2014

    • Author(s)
      Hayashi R, Kozuka M, Shishido S, Kakiuchi Y, Henmi A, Okata H, Sasano Y
    • Organizer
      The 6th International Symposiumfor Interface Oral Health Science
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] ANALYSIS OF CALCIFICATION IN BONE HEALING WITH A STANDARDISED RAT CALVARIAL DEFECT MODEL2013

    • Author(s)
      Okata H, Nakamura M, Henmi A, Yamaguchi S, Mikami Y, Shimauchi H, Sasano Y
    • Organizer
      ASahct2013 International SymposiumAnatomical Science for advance in health and clinical therapy
    • Place of Presentation
      Sendai, Japan
    • Related Report
      2013 Research-status Report
  • [Presentation] 骨発生・成長における石灰化過程の解析2013

    • Author(s)
      逸見 晶子、大方 広志、穴田 貴久、三上 靖人、鈴木 治、笹野 泰之
    • Organizer
      日本解剖学会 第59回東北・北海道連合支部学術集会
    • Place of Presentation
      札幌市
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット頭蓋骨発生・成長過程における骨基質石灰化の成熟に関する検討2013

    • Author(s)
      逸見 晶子、大方 広志、三上 靖人、鈴木 治、笹野 泰之
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] ラット下顎骨発生における石灰化2013

    • Author(s)
      林 利華、狐塚 雅弘、宍戸 駿一、柿内 裕輔、逸見 晶子、大方 広志、笹野 泰之
    • Organizer
      第55回歯科基礎医学会学術大会・総会
    • Place of Presentation
      岡山市
    • Related Report
      2013 Research-status Report
  • [Presentation] 硬組織の発生・修復における細胞外基質リモデリングと石灰化2013

    • Author(s)
      笹野 泰之、逸見 晶子,大方 広志、三上 靖人、中村 恵
    • Organizer
      第118回日本解剖学会総会・全国学術集会 研究集会・懇話会
    • Place of Presentation
      香川県高松市
    • Related Report
      2012 Research-status Report
  • [Presentation] 骨欠損修復における骨基質の石灰化に関する検討2012

    • Author(s)
      大方広志、中村 恵、逸見晶子、島内英俊、笹野泰之
    • Organizer
      第54回歯科基礎医学会学術大会
    • Place of Presentation
      福島県郡山市
    • Related Report
      2012 Research-status Report
  • [Presentation] 骨密度と構成元素の解析を利用した修復骨基質の石灰化の検討2012

    • Author(s)
      大方広志、中村 恵、逸見晶子、島内英俊、笹野泰之
    • Organizer
      第58回東北・北海道連合支部学術集会
    • Place of Presentation
      山形県山形市
    • Related Report
      2012 Research-status Report
  • [Presentation] 実験的骨欠損における修復骨形成過程の石灰化進行に関する検討2012

    • Author(s)
      大方広志、中村 恵、逸見晶子、島内英俊、笹野泰之
    • Organizer
      第117回日本解剖学会総会・全国学術集会
    • Place of Presentation
      甲府市
    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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