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Establishment of iPS cell lines from dental pulp cells using Sendai virus vectors

Research Project

Project/Area Number 23592697
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionGifu University

Principal Investigator

TEZUKA Ken-ichi  岐阜大学, 医学(系)研究科(研究院), 准教授 (50236973)

Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Keywords歯髄細胞 / iPS細胞 / センダイウイルスベクター / HLAタイプ / 再生医療 / ヒト白血球抗原 / Zinc Finger Nuclease / Human Leucocyte Antigen / 人工多能性幹細胞 / 無血清培地
Research Abstract

For application of iPS cells for clinical use, safer methods without vector integration are being established. Recently, we used episomal plasmid vector system for this purpose, and succeeded to establish multiple lines in collaboration with Kyoto University. One of the lines were submitted to RIKEN BRC and started to be distributed to other researchers. In this research, we tried to use Sendai virus vector systems to establish iPS cells homozygous for human leukocyte antigens (HLAs). We also tried serum-free method for culturing the cells, and succeeded to establish multiple iPS cell lines.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (34 results)

All 2014 2013 2012 2011 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (25 results) (of which Invited: 18 results) Book (2 results) Patent(Industrial Property Rights) (4 results) (of which Overseas: 3 results)

  • [Journal Article] Dual origin of melanocytes defined by Sox 1 expression and their region-specific distribution in mammalian skin.2013

    • Author(s)
      Naoko Yoshimura, Tsutomu Motohashi, Hitomi Aoki, Ken-ichi Tezuka, Natsuki Watanabe, Takanori Wakaoka, Takumi Era, Takahiro Kunisada
    • Journal Title

      Development, Growth and Differentiation

      Volume: 55 Issue: 2 Pages: 270-281

    • DOI

      10.1111/dgd.12034

    • NAID

      40019734976

    • Related Report
      2013 Final Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Hypoxia-enhanced Derivation of iPSCs from Human Dental Pulp Cells2013

    • Author(s)
      K.Iida,T.Kawaguchi,M.Hada,M.Yuriguchi,H.Aoki,NTamaoki,D.Hatakeyama,T.Kunisada,T.Shibata,K.Tezuka
    • Journal Title

      Journal of Dental Research

      Volume: 92 Issue: 10 Pages: 905-910

    • DOI

      10.1177/0022034513502204

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] A more efficient method to generate integration-free human iPS cells.2011

    • Author(s)
      K Okita, Y Matsumura, Y Sato, A Okada, A Morizane, S Okamoto, H Hong, M Nakagawa, K Tanabe, K Tezuka, T Shibata, T Kunisada, M Takahashi, J Takahashi, H Saji, S Yamanaka
    • Journal Title

      Nature Methods

      Volume: 8 Pages: 409-412

    • NAID

      120002949763

    • Related Report
      2011 Research-status Report
    • Peer Reviewed
  • [Presentation] 岐阜歯髄細胞コレクション〜未来の再生医療を支えるためにできること〜2014

    • Author(s)
      手塚建一
    • Organizer
      岐阜歯科学会40周年記念大会
    • Place of Presentation
      岐阜
    • Year and Date
      2014-01-12
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking - A low cost human cell resource for regenerative medicine-2013

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      5th Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      広島
    • Year and Date
      2013-10-13
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 親知らずからiPS細胞~歯の中にあるたからもの~2013

    • Author(s)
      手塚建一
    • Organizer
      岐阜市母子寡婦福祉大会講演会
    • Place of Presentation
      岐阜
    • Year and Date
      2013-09-23
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 再生医療資源としての歯髄細胞利用2013

    • Author(s)
      手塚建一
    • Organizer
      歯科基礎医学会サテライトシンポジウム
    • Place of Presentation
      岡山
    • Year and Date
      2013-09-20
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 岐阜県が日本を救う?~親知らずからiPS細胞~2013

    • Author(s)
      手塚建一
    • Organizer
      岐阜大学フェア
    • Place of Presentation
      高山
    • Year and Date
      2013-08-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking2013

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      6th International Symposiumon the Biology of Tertrate- Resistant Acid Phosphatase
    • Place of Presentation
      神戸
    • Year and Date
      2013-05-27
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking2013

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      CiRA Symposium 2013
    • Place of Presentation
      京都
    • Year and Date
      2013-03-11
    • Related Report
      2013 Final Research Report
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking2013

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      Kyudai Oral Bioscience 2013 -7th International Symposium
    • Place of Presentation
      福岡
    • Year and Date
      2013-03-08
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 日本人HLAハプロタイプホモ歯髄細胞コレクション2013

    • Author(s)
      手塚建一
    • Organizer
      明海大学歯学部研修会
    • Place of Presentation
      埼玉
    • Year and Date
      2013-02-22
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] 日本人HLAハプロタイプホモ歯髄細胞コレクション2013

    • Author(s)
      手塚建一
    • Organizer
      名古屋大学IGER SEMINAR
    • Place of Presentation
      名古屋
    • Year and Date
      2013-01-17
    • Related Report
      2013 Final Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking2013

    • Author(s)
      Ken-ichi Tezuka, Tomoko Kawaguchi, Naritaka Tamaoki, Kazuki Iida, Hitomi Aoki, Toshiyuki Shibata, Takahiro Kunisada
    • Organizer
      2013 Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      Baltimore
    • Related Report
      2013 Final Research Report
  • [Presentation] Dental Pulp Cells as Source for iPS Cell Banking.2013

    • Author(s)
      Ken-ichi Tezuka, Tomoko Kawaguchi, Naritaka Tamaoki, Kazuki Iida, Hitomi Aoki, Toshiyuki Shibata, Takahiro Kunisada
    • Organizer
      2013 Annual Meeting of the American Society for Bone and Mineral Research
    • Place of Presentation
      ボルチモア
    • Related Report
      2013 Annual Research Report
  • [Presentation] Generation of integration-free human dental-pulp-derived iPS cells from HLA-homozygous donors using Sendai virus vectors2012

    • Author(s)
      Noemi Fusaki, Hiroshi Ban, Masataka Hada, Akihiro Iida, Mamoru Hasegawa, Kunisada and Ken-ichi Tezuka
    • Organizer
      10th Annual Meeting of International Society for Stem Cell Research
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2013 Final Research Report
  • [Presentation] Generation of integration-free human dental-pulp-derived iPS cells from HLA-homozygous donors using Sendai virus vectors.2012

    • Author(s)
      Noemi Fusaki, Hiroshi Ban, Masataka Hada, Akihiro Iida, Mamoru Hasegawa, Kunisada and Ken-ichi Tezuka
    • Organizer
      10th Annual Meeting of International Society for Stem Cell Research
    • Place of Presentation
      Yokohama, Japan
    • Related Report
      2012 Research-status Report
  • [Presentation] iPS細胞バンクのための歯髄細胞バンク構築2011

    • Author(s)
      手塚 建一
    • Organizer
      第65回日本口腔科学会学術集会(招待講演)
    • Place of Presentation
      タワーホール船堀
    • Related Report
      2011 Research-status Report
  • [Presentation] 岐阜歯髄細胞コレクション~未来の再生医療を支えるためにできること~

    • Author(s)
      手塚 建一
    • Organizer
      岐阜歯科学会40周年記念大会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking -A low cost human cell resource for regenerative medicine-

    • Author(s)
      Tezuka K.
    • Organizer
      5th Hiroshima Conference on Education and Science in Dentistry
    • Place of Presentation
      広島
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 親知らずからiPS細胞~歯の中にあるたからもの~

    • Author(s)
      手塚 建一
    • Organizer
      岐阜市母子寡婦福祉大会 講演会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 再生医療資源としての歯髄細胞利用

    • Author(s)
      手塚 建一
    • Organizer
      歯科基礎医学会 サテライトシンポジウム
    • Place of Presentation
      岡山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 岐阜県が日本を救う?~親知らずからiPS細胞~

    • Author(s)
      手塚 建一
    • Organizer
      岐阜大学フェア
    • Place of Presentation
      高山
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Dental Pulp Cells as Source for iPS Cell Banking.

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      6th International Symposium on the Biology of Tertrate-Resistant Acid Phosphatase
    • Place of Presentation
      神戸
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] 日本人HLAハプロタイプホモ歯髄細胞コレクション

    • Author(s)
      手塚 建一
    • Organizer
      名古屋大学 IGER SEMINAR
    • Place of Presentation
      名古屋
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] 日本人HLAハプロタイプホモ歯髄細胞コレクション

    • Author(s)
      手塚 建一
    • Organizer
      明海大学歯学部研修会
    • Place of Presentation
      埼玉
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking.

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      Kyudai Oral Bioscience 2013 -7th International Symposium
    • Place of Presentation
      福岡
    • Related Report
      2012 Research-status Report
    • Invited
  • [Presentation] Dental Pulp Cells as Souce for iPS Cell Banking.

    • Author(s)
      Ken-ichi Tezuka
    • Organizer
      CiRA Symposium 2013
    • Place of Presentation
      京都
    • Related Report
      2012 Research-status Report
  • [Book] 再生医療叢書第8巻2012

    • Author(s)
      手塚建一、柴田敏之、國貞隆弘
    • Publisher
      朝倉書店
    • Related Report
      2013 Final Research Report
  • [Book] 再生医療叢書 第8巻2012

    • Author(s)
      手塚建一、柴田敏之、國貞隆弘
    • Total Pages
      185
    • Publisher
      朝倉書店
    • Related Report
      2012 Research-status Report
  • [Patent(Industrial Property Rights)] Efficient method for establishing induced pluripotent stem cells2013

    • Inventor(s)
      Tezuka K et al
    • Industrial Property Rights Holder
      岐阜大学、京都大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-01-22
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 人工多能性幹細胞の作製方法2013

    • Inventor(s)
      手塚建一他7名
    • Industrial Property Rights Holder
      岐阜大学、産総研
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-176647
    • Filing Date
      2013-08-28
    • Related Report
      2013 Final Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 人工多能性幹細胞の作製方法2013

    • Inventor(s)
      手塚建一他7名
    • Industrial Property Rights Holder
      手塚建一他7名
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2013-176647
    • Filing Date
      2013-08-28
    • Related Report
      2013 Annual Research Report
  • [Patent(Industrial Property Rights)] Efficient method for establishing induced pluripotent stem cells.2013

    • Inventor(s)
      Tezuka K et al.
    • Industrial Property Rights Holder
      岐阜大学、京都大学
    • Industrial Property Rights Type
      特許
    • Filing Date
      2013-01-22
    • Related Report
      2012 Research-status Report
    • Overseas

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Published: 2011-08-05   Modified: 2019-07-29  

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