Establishment of iPS cell lines from dental pulp cells using Sendai virus vectors
| Project/Area Number |
23592697
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Morphological basic dentistry
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| Research Institution | Gifu University |
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Principal Investigator |
TEZUKA Ken-ichi 岐阜大学, 医学(系)研究科(研究院), 准教授 (50236973)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 歯髄細胞 / iPS細胞 / センダイウイルスベクター / HLAタイプ / 再生医療 / ヒト白血球抗原 / Zinc Finger Nuclease / Human Leucocyte Antigen / 人工多能性幹細胞 / 無血清培地 |
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| Research Abstract |
For application of iPS cells for clinical use, safer methods without vector integration are being established. Recently, we used episomal plasmid vector system for this purpose, and succeeded to establish multiple lines in collaboration with Kyoto University. One of the lines were submitted to RIKEN BRC and started to be distributed to other researchers. In this research, we tried to use Sendai virus vector systems to establish iPS cells homozygous for human leukocyte antigens (HLAs). We also tried serum-free method for culturing the cells, and succeeded to establish multiple iPS cell lines.
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] A more efficient method to generate integration-free human iPS cells.2011
Author(s)
K Okita, Y Matsumura, Y Sato, A Okada, A Morizane, S Okamoto, H Hong, M Nakagawa, K Tanabe, K Tezuka, T Shibata, T Kunisada, M Takahashi, J Takahashi, H Saji, S Yamanaka
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Journal Title
Nature Methods
Volume: 8
Pages: 409-412
NAID
Related Report
Peer Reviewed
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