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Mechanism of automutanolysin conferring substrate selectivity on cariogenic streptococci

Research Project

Project/Area Number 23592702
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionHiroshima University

Principal Investigator

HAYASHI Ikue  広島大学, 医歯薬保健学研究院, 助教 (00346503)

Co-Investigator(Kenkyū-buntansha) OHARA Masaru  広島大学, 病院, 助教 (80253095)
KATOU Fuminori  広島大学, 医歯薬保健学研究院, 助教 (70452589)
Co-Investigator(Renkei-kenkyūsha) SUGAI Motoyuki  広島大学, 医歯薬保健学研究院, 教授 (10201568)
Research Collaborator THANYASRISUNG Panida  広島大学, 医歯薬保健学研究院, 留学生
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords溶菌酵素 / ペプチドグリカン / 基質特異性 / 口腔レンサ球菌 / う喰
Research Abstract

Automutanolysin (Aml) produced by S.mutans selectively lyses S. mutans and S. sobrinus but not other oral streptococci. To unveil the mechanism how Aml draws this distinction, we analysed the peptidoglycan structures of oral streptococci. We made various recombinant Aml. It consists of two domains: a C-terminal catalytic domain and an N-terminus containing five repeats presumably acting as a cell wall targeting domain. A full length of targeting domain revealed the highest binding ability to the tested strain. Moreover, it clearly distinguished cariogenic bacteria from other oral streptococci. Comparison of the muropeptide profiles of oral streptococci strains with different level of susceptibility to Aml appeared to be correlated with ratio of muropeptides carrying a Penta-peptide chain for Aml resistance. Binding domain of Aml clearly differentiate cariogenic streptococci from others by recognizing peptidoglycan structure.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (3 results)

All 2013 2012

All Journal Article (1 results) Presentation (2 results)

  • [Journal Article] The Listeria monocytogenes serotype 4b autolysin IspC has N-acetylglucosaminidase activity2012

    • Author(s)
      Ronholm J., Wang L., Hayashi I., Sugai M., Zhang Z., Cao X., Lin M.
    • Journal Title

      Glycobiology

      Volume: 22(10) Pages: 1311-1320

    • Related Report
      2013 Final Research Report
  • [Presentation] The N-terminal cell wall targeting domain of automutanolysin confers substrate selectivity on cariogenic streptococci2013

    • Author(s)
      P. Thanyasrisung, I. Hayashi, T. Fujiwara, K. Tsuruda, M. Sugai
    • Organizer
      2^<nd> Meeting of the International Association for Dental Research–Asia Pacific Region
    • Place of Presentation
      Bangkok
    • Related Report
      2013 Final Research Report
  • [Presentation] The N-terminal cell wall targeting domain of automutanolysin confers substrate selectivity on cariogenic streptococci2013

    • Author(s)
      P Thanyasrisung, I. Hayashi, T. Fujiwara, K.Tsuruda, M.Sugai
    • Organizer
      2nd Meeting of the International Association for Dental Research
    • Place of Presentation
      Bangkok、Thailand
    • Related Report
      2013 Annual Research Report

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Published: 2011-08-05   Modified: 2019-07-29  

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