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Temporo-spatial interactions among blood vessels, nerve axons and myogenic cells govern the guidance of hypoglossal nerve in mouse tongue primordium

Research Project

Project/Area Number 23592716
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Morphological basic dentistry
Research InstitutionThe Nippon Dental University

Principal Investigator

SATO KAORI  日本歯科大学, 生命歯学部, 講師 (90287772)

Co-Investigator(Kenkyū-buntansha) TAYA Yuji  日本歯科大学, 生命歯学部, 准教授 (30197587)
SOENO Yuuichi  日本歯科大学, 生命歯学部, 講師 (70350139)
SHIMAZU Yoshihito  日本歯科大学, 生命歯学部, 講師 (10297947)
AOBA Takaaki  日本歯科大学, 生命歯学部, 教授 (30028807)
Project Period (FY) 2011 – 2013
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords歯学 / 病理学 / 舌発生 / 血管・神経網 / 筋細胞系譜 / 軸索誘導 / ガイダンス因子 / ガイダンス機構 / 遺伝子発現
Research Abstract

We aimed to investigate the guidance mechanism of growing hypoglossal nerve axons in mouse tongue development. 3D reconstruction was conducted using 150~600 serial sections. All serial sections, which were obtained from ICR mouse embryos at E9.5~11.5, were dual-immunolabeled with Pecam1, Desmin or Pgp9.5-Gap43 antibodies. The achieved 3D reconstructs warranted superior positional accuracy to trace the long-range connectivity of blood vessels and individual cranial nerve axons. The hypoglossal nerve axons, which uniquely underwent long-range migration toward tongue primordium, were in close contact with tongue myogenic cells that originated from the occipital somites, and the blood capillaries were localized at the vicinity of the tip of hypoglossal nerve axons. The analysis of gene expression and protein localization revealed that Cxcl12 and Cxcr4 signals, which originated from tongue myogenic cells and blood capillaries, may act as the axon guidance factors of the hypoglossal nerve.

Report

(4 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Research-status Report
  • 2011 Research-status Report
  • Research Products

    (33 results)

All 2014 2013 2012 2011 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (22 results) Book (3 results) Remarks (3 results)

  • [Journal Article] Molecular contribution to cleft palate production in cleft lip mice2014

    • Author(s)
      Sasaki Y, Taya Y, Saito K, Fujita K, Aoba T, and Fujiwara T
    • Journal Title

      Congenital Anomalies

      Volume: 54 Issue: 2 Pages: 94-99

    • DOI

      10.1111/cga.12038

    • NAID

      120006986612

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Molecular signaling at the fusion stage of the mouse mandibular arch : involvement of insulin-like growth factor family2013

    • Author(s)
      Fujita K, Taya Y, Shimazu Y, Aoba T, Soeno Y
    • Journal Title

      Int J Dev Biol

      Volume: 57 Pages: 399-406

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular signaling at the fusion stage of the mouse mandibular arch: involvement of insulin-like growth factor family.2013

    • Author(s)
      Kazuya Fujita
    • Journal Title

      Int. J. Dev. Biol.

      Volume: 57 Issue: 5 Pages: 1-8

    • DOI

      10.1387/ijdb.120110ys

    • Related Report
      2013 Annual Research Report 2012 Research-status Report
    • Peer Reviewed
  • [Journal Article] Generation of a mouse model with down-regulated U50 snoRNA (SNORD50) expression and its organ-specific phenotypic modulation2013

    • Author(s)
      Soeno Y, Fujita K, Kudo T, Asagiri M, Kakuta S, Taya Y, Shimazu Y, Sato K, Tanaka-Fujita R, Kubo S, Iwakura Y, Nakamura Y, Mori S, and Aoba T
    • Journal Title

      PLoS ONE

      Volume: 8 Issue: 8 Pages: e72105-e72105

    • DOI

      10.1371/journal.pone.0072105

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 腫瘍微小環境と口腔癌浸潤:舌癌YK分類と三次元癌胞巣構造(口腔癌の浸潤:マクロ・ミクロ・モレキュラー).2012

    • Author(s)
      島津徳人
    • Journal Title

      日本口腔腫瘍学会誌

      Volume: 24 Pages: 77-87

    • Related Report
      2012 Research-status Report
    • Peer Reviewed
  • [Presentation] マウス舌形態形成におけるリンパ管発生と分子制御2013

    • Author(s)
      田谷雄二,藤田和也,添野雄一,島津徳人,佐藤かおり,青葉孝昭
    • Organizer
      J Oral Biosci. 55(Suppl)
    • Place of Presentation
      128 (No.O-84)
    • Related Report
      2013 Final Research Report
  • [Presentation] 脈管構造の組織立体構築とVirtual Relity観察2013

    • Author(s)
      島津徳人,田谷雄二,添野雄一,白子要一,藤田和也,佐藤かおり,青葉孝昭
    • Organizer
      J Oral Biosci. 55(Suppl)
    • Place of Presentation
      110 (No.O-84)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス舌脈管ネットワークの組織立体構築と仮想空間での動的観察2013

    • Author(s)
      島津徳人、添野雄一、白子要一、藤田和也、田谷雄二、中右かよ、佐藤かおり、青葉孝昭
    • Organizer
      日本解剖学会抄録集
    • Place of Presentation
      75(1):102
    • Related Report
      2013 Final Research Report
  • [Presentation] Three-dimensional comprehension of developing blood vessel and nerve networks in the craniofacial region of mouse embryos2013

    • Author(s)
      Taya Y, Shimazu Y, Fujita K, Soeno Y, Sato K, and Aoba T
    • Organizer
      日本解剖学会抄録集
    • Place of Presentation
      75(1): 151
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス舌形態形成におけるリンパ管発生と分子制御2013

    • Author(s)
      田谷雄二,藤田和也,添野雄一,島津徳人,佐藤かおり,青葉孝昭
    • Organizer
      歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岡山県 岡山コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] 脈管構造の組織立体構築とVirtual Relity観察2013

    • Author(s)
      島津徳人、田谷雄二、添野雄一、白子要一、藤田和也、佐藤かおり、青葉孝昭
    • Organizer
      歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      岡山県 岡山コンベンションセンター
    • Related Report
      2013 Annual Research Report
  • [Presentation] Myoblast-differentiation and mRNA/miRNA expression in developmental mouse tongue2012

    • Author(s)
      Fujita K, Soeno Y, Taya Y, Shimazu Y, Sato K, and Aoba T
    • Organizer
      PROGRAM BOOK 41st Annual meeting & exhibition of the American Assosiation for Dental Research
    • Related Report
      2013 Final Research Report
  • [Presentation] Three-dimensional visualization of developing vascular-nervous networks in embryonic mice2012

    • Author(s)
      Taya Y, Shimazu Y, Fujita K, Soeno Y, Sato K, Aoba T
    • Organizer
      2012 Sino-Japan Dental Conference Proceedings
    • Related Report
      2013 Final Research Report
  • [Presentation] Embryonic morphogenesis and organization of vascular and nervous networks in mouse craniofacial region, Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology2012

    • Author(s)
      Taya Y, Shimazu Y, Fujita K, Soeno Y, Sato K, Aoba T
    • Organizer
      Joint Meeting of The 45th Annual Meeting of the Japanese Society of Developmental Biologists & The 64th Annual Meeting of the Japan Society for Cell Biology, Program & Abstract Book
    • Place of Presentation
      176 (P1-124)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス舌発生における舌筋前駆細胞の移住は舌下神経の軸索誘導に働く2012

    • Author(s)
      田谷雄二,島津徳人,佐藤かおり,藤田和也,添野雄一,青葉孝昭
    • Organizer
      J Oral Biosci
    • Place of Presentation
      54(Suppl): 106 (No.O-91)
    • Related Report
      2013 Final Research Report
  • [Presentation] Myoblast-differentiation and mRNA/miRNA expression in developmental mouse tongue2012

    • Author(s)
      Fujita Kazuya
    • Organizer
      41st Annual Meeting and Exhibition of the American Association for Dental Research
    • Place of Presentation
      Tampa, Florida, USA.
    • Related Report
      2011 Research-status Report
  • [Presentation] マウス顎顔面領域における器官発生の分子制御:下顎突起癒合期における脈管網・神経網の構築2011

    • Author(s)
      田谷雄二,藤田和也,島津徳人,佐藤かおり,添野雄一,青葉孝昭
    • Organizer
      J.Oral Biosci
    • Place of Presentation
      53(Suppl):180 (No.P2-32)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス舌初期発生における筋芽細胞分化のタイミングと遺伝子発現制御2011

    • Author(s)
      藤田和也,田谷雄二,佐藤かおり,島津徳人,添野雄一,青葉孝昭
    • Organizer
      J.Oral Biosci
    • Place of Presentation
      53(Suppl):180 (No.P2-32)
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス舌初期発生と筋芽細胞分化の分子制御:miRNA・タンパク質の発現局在2011

    • Author(s)
      藤田和也,添野雄一,田谷雄二,島津徳人,佐藤かおり,青葉孝昭
    • Organizer
      第34回日本分子生物学会年会MBSJ2011プログラム
    • Related Report
      2013 Final Research Report
  • [Presentation] マウス顎顔面領域における器官発生の分子制御:下顎突起癒合期における脈管網・神経網の構築2011

    • Author(s)
      田谷雄二
    • Organizer
      第53回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Related Report
      2011 Research-status Report
  • [Presentation] マウス舌初期発生における筋芽細胞分化のタイミングと遺伝子発現制御2011

    • Author(s)
      藤田和也
    • Organizer
      第53回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      長良川国際会議場(岐阜県)
    • Related Report
      2011 Research-status Report
  • [Presentation] マウス舌初期発生と筋芽細胞分化の分子制御:miRNA・タンパク質の発現局在2011

    • Author(s)
      藤田和也
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Related Report
      2011 Research-status Report
  • [Presentation] Three-dimensional visualization of developing vascular-nervous networks in embryonic mice.

    • Author(s)
      Yuji Taya
    • Organizer
      2012 Sino-Japan Dental Conference
    • Place of Presentation
      四川大学(中国)
    • Related Report
      2012 Research-status Report
  • [Presentation] Web公開Image-Jによる癌浸潤先端の組織立体構築.

    • Author(s)
      島津徳人
    • Organizer
      第101回日本病理学会総会
    • Place of Presentation
      京王プラザホテル(東京都)
    • Related Report
      2012 Research-status Report
  • [Presentation] Embryonic morphogenesis and organization of vascular and nervous networks in mouse craniofacial region.

    • Author(s)
      Yuji Taya
    • Organizer
      第64回日本細胞生物学会・第45回日本発生生物学会合同大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Related Report
      2012 Research-status Report
  • [Presentation] Three-dimensional Visualization of Tumor Microenvironment Based on multiplex-immunolabeled Color Images of Serial Histological Sections.

    • Author(s)
      Yoshihito Shimazu
    • Organizer
      14th INTERNATIONAL CONGRESS OF HISTOCHEMISTRY AND CYTOCHEMISTRY
    • Place of Presentation
      国立京都国際会館(京都府)
    • Related Report
      2012 Research-status Report
  • [Presentation] マウス舌発生における舌筋前駆細胞の移住は舌下神経の軸索誘導に働く.

    • Author(s)
      田谷雄二
    • Organizer
      第54回歯科基礎医学会学術大会ならびに総会
    • Place of Presentation
      奥羽大学(福島県)
    • Related Report
      2012 Research-status Report
  • [Book] 日本歯科大学病理学講座編(青葉孝昭監修):歯学生のための最新・病態病理学入門2014

    • Author(s)
      佐藤かおり
    • Publisher
      株式会社キタ・メディア,東京
    • Related Report
      2013 Final Research Report
  • [Book] 歯学生のための最新・病態病理学入門2014

    • Author(s)
      青葉孝昭 監修 佐藤かおり 編集
    • Total Pages
      177
    • Publisher
      株式会社キタ・メディア
    • Related Report
      2013 Annual Research Report
  • [Book] 今すぐ始める組織立体構築―ImageJ/Fijiの活用プロトコール―2013

    • Author(s)
      島津徳人 編、青葉孝昭 監修、佐藤かおり・田谷雄二・添野雄一 執筆
    • Total Pages
      22
    • Publisher
      日本ビジュアルサイエンス社
    • Related Report
      2013 Annual Research Report
  • [Remarks] 日本歯科大学病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2013 Annual Research Report
  • [Remarks] 日本歯科大学病理学講座HP

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2012 Research-status Report
  • [Remarks]

    • URL

      http://www.ndu.ac.jp/~pathhome/

    • Related Report
      2011 Research-status Report

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Published: 2011-08-05   Modified: 2019-07-29  

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