Epigenetic mechanism of transcription factor DEC2 regulation in cancer cells
Project/Area Number |
23592717
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Nihon University |
Principal Investigator |
BHAWAL Ujjal 日本大学, 松戸歯学部, 助教 (50433339)
|
Co-Investigator(Kenkyū-buntansha) |
SATO Fuyuki 弘前大学, 医学部, 助教 (60400131)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
Keywords | 転写因子 / DEC2 / エピジェネティック解析 / 放射線照射 / エピジェネティク解析 / 2-Dec / in vivoイメージング |
Research Abstract |
Basic-helix-loop-helix (bHLH) transcription factor DEC2 (BHLHB3/Sharp1) regulates cell proliferation and apoptosis. In this study, we showed that cell treatment with the demethylating agent 5-aza-2-deoxycytidine and histone-deacetylating agent TSA resulted in the recovery of DEC2 mRNA and protein expression in some oral cancer cell lines. Hypermethylated CpG island sequences encompassing the DEC2 gene promoter were identified. Furthermore, mice implanted with oral cancer cell lines induced the expression of DEC2 and other hypoxia-related genes. Thus, our data showed the state of hypermethylation of CpG islands in the DEC2 gene promoter region and the irradiation effect on DEC2 expression in oral cancer cells.
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Report
(4 results)
Research Products
(74 results)
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[Journal Article] Silk fibroin-based scaffolds for bone regeneration2013
Author(s)
Kuboyama N, Kiba H, Arai K, Uchida R, Tanimoto Y, Bhawal UK, Abiko Y, Miyamoto S, Knight D, Asakura T, Nishiyama N.
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Journal Title
J Biomed Mater Res B Appl Biomater
Volume: Vol.101
Issue: 2
Pages: 295-302
DOI
Related Report
Peer Reviewed
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[Journal Article] Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver2012
Author(s)
Sato F, Sato H, Jin D, Bhawal UK, Wu Y, Noshiro M, Kawamoto T, Fujimoto K, Seino H, Morohashi S, Kato Y, Kijima H.
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Journal Title
Biochemical Biophysics Research Communication
Volume: 419(2)
Pages: 441-446
Related Report
Peer Reviewed
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[Journal Article] Involvement of HMGB1 and RAGE in IL-1β-induced gingival inflammation2012
Author(s)
Ito Y, Bhawal UK, Sasahira T, Toyama T, Sato T, Matsuda D, Nishikiori H, Kobayashi M, Sugiyama M, Hamada N, Arakawa H, Kuniyasu H.
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Journal Title
Archives of Oral Biology
Volume: 57(1)
Pages: 73-80
Related Report
Peer Reviewed
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[Journal Article] Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D12011
Author(s)
Bhawal UK, Sato F, Arakawa Y, Fujimoto K, Kawamoto T, Tanimoto K, Ito Y, Sasahira T, Sakurai T, Kobayashi M, Kashima I, Kijima H, Kuniyasu H, Abiko Y, Kato Y, Sato S.
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Journal Title
Journal of Pathology
Volume: 224(3)
Pages: 420-429
Related Report
Peer Reviewed
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