Differentiation of iPS cells into salivary glands
Project/Area Number |
23592718
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
Morphological basic dentistry
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Research Institution | Showa University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
SAITO Ichiro 鶴見大学, 歯学部, 教授 (60147634)
INOUE Hiroko 日本薬科大学, 薬学部, 准教授 (50367306)
RYO Koufuchi 鶴見大学, 歯学部, 講師 (10298268)
|
Project Period (FY) |
2011 – 2013
|
Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
|
Keywords | 唾液分泌障害 / 再生医療 / iPS細胞 / 唾液腺 |
Research Abstract |
Salivary gland hypofunction causes not only various oral diseases but also a decrease in quality of life by systemic diseases, such as aspiration pneumonia. However, to date, there is no efficient method to treat the patients with severe symptoms. The purpose of this study is to apply a regenerative medicine using induced pluripotent (iPS) cells for these patients. We established stromal cells from salivary glands of mice at embryonic day 11.5 and 13.5, respectively. iPS cells were differentiated into oral epithelium using LCA (large-cell aggregate)-SFEBq method. These iPS cells were cultured on the established feeder cells and specific gene expressions for salivary glands were detected by RT-PCR. Consequently, gene expressions of Aqp5, M3AchR, and beta2AdR, which are highly expressed in salivary glands, were increased in these iPS cells. Therefore, these results suggested that the present method could be applied for the differentiation of iPS cells into salivary gland cells.
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Report
(4 results)
Research Products
(15 results)
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[Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes2013
Author(s)
Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
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Journal Title
Related Report
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[Journal Article] Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma2013
Author(s)
Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K
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Journal Title
Exp Cell Res
Volume: 319
Pages: 1220-8
Related Report
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[Journal Article] Downregulation of carbonic anhydrase IX promotes Col10a1 expression in chondrocytes2013
Author(s)
Maruyama T, Miyamoto Y, Yamamoto G, Yamada A, Yoshimura K, Suzawa T, Takami M, Akiyama T, Hoshino M, Iwasa F, Ikumi N, Tachikawa T, Mishima K, Baba K, Kamijo R
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Journal Title
PLoS ONE
Volume: Vol.8
Issue: 2
Pages: e56984-e56984
DOI
Related Report
Peer Reviewed
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[Journal Article] Lin28a is a putative factor in regulating cancer stem cell-like properties in side population cells of oral squamous cell carcinoma.2013
Author(s)
Hayashi S, Tanaka J, Okada S, Isobe T, Yamamoto G, Yasuhara R, Irie T, Akiyama C, Kohno Y, Tachikawa T, Mishima K.
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Journal Title
Exp Cell Res
Volume: 319
Issue: 8
Pages: 1220-1228
DOI
Related Report
Peer Reviewed
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[Journal Article] Transplantation of side population cells restores the function of damaged exocrine glands through clusterin2012
Author(s)
Mishima K., Inoue H., Nishiyama T., Mabuchi Y., Amano Y., Ide F., Matsui M., Yamada H., Yamamoto G., Tanaka J., Yasuhara R., Sakurai T., Lee M., Chiba K., Sumimoto H., Kawakami Y., Matsuzaki Y., Tsubota K., Saito I
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Journal Title
Stem Cells
Volume: 30
Issue: 9
Pages: 1925-37
DOI
Related Report
Peer Reviewed
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