| Project/Area Number |
23592728
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Tohoku University |
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Principal Investigator |
MIYAZAKI Tatsuya 東北大学, 歯学研究科(研究院), 大学院非常勤講師 (90538638)
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| Co-Investigator(Kenkyū-buntansha) |
SUZUKI Osamu 東北大学, 大学院歯学研究科, 教授 (60374948)
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| Co-Investigator(Renkei-kenkyūsha) |
ANADA Takahisa 東北大学, 大学院歯学研究科, 准教授 (30398466)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2012: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | コンドロイチン硫酸 / インテグリン / オステオアクチビン / 破骨細胞 / 骨形成 / ヘパラン硫酸 / 骨代謝 / 骨芽細胞 |
|---|
| Research Abstract |
Chondroitin sulfate E enhances osteoblast differentiation and inhibits osteoclast differentiation. As a mechanism of action, it was demonstrated that CS-E bound to adhesion molecules such as both osteoactivin (OA) and its receptor, integrin. And also CS-E inhibited the osteoclast differentiation by blocking the interaction of OA to integrin and heparan sulfate. On the other hands, osteoblast produced biglycan core protein and E-unit of CS. The amount of these molecules transiently increased before osteoblast differentiation (mineralization). These results indicated that not only the exogenously-added CS-E but also endogenous CS contributed the differentiation of the cells. Based on the results in the present study, we will further investigate the potentiality of CS-E as an application to bone regenerating biomaterials.
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