Analysis of molecular mechanism of muscle heterotopic ossification by forming a network with CCN family proteins

• Project/Area Number: 23592732
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Okayama University
• Principal Investigator: NISHIDA Takashi 岡山大学, 医歯(薬)学総合研究科, 助教 (30322233)
• Co-Investigator(Kenkyū-buntansha): TAKIGAWA Masaharu 岡山大学, 大学院医歯薬学総合研究科, 教授 (20112063) ; KUBOTA Satoshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (90221936) ; HATTORI Takako 岡山大学, 大学院医歯薬学総合研究科, 助教 (00228488) ; AOYAMA Eriko 岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000); Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: CCN2/CTGF / C2C12細胞 / 骨格筋分化 / 異所性骨化 / BMP2 / Ccn2欠損筋芽細胞 / 筋芽細胞の増殖 / 筋芽細胞の分化 / 筋肉内異所性骨化 / マウス筋芽細胞 / CCN2 / CCNファミリー / 筋管形成 / 骨芽細胞様変化 / CCN2/結合組織成長因子 / 炎症性シグナル

Research Abstract

The protein production of CCN2 (also known as Connective tissue growth factor) was increased in mouse myoblastic cell line C2C12 by treatment with tumor necrosis factor-a, which is produced upon inflammation. CCN2 promoted cell proliferation and the protein production of MyoD in C2C12 cells. Consistent with these findings, in vivo analyses with Ccn2-deficient skeletal muscle showed the decreased PCNA staining and muscle hypoplasia. Furthermore, bone morphogenetic protein (BMP)2-induced Runx2 and Osterix gene expression levels were significantly decreased in C2C12 cells co-treated with CCN2.

Research Products

• [Journal Article] CCN2 as a novel molecule supporting energy metabolism of chondrocytes. (2014)
  • Author(s): Maeda-Uematsu A, Kubota S, Kawaki H, Kawata K, Miyake Y, Hattori T, Nishida T, Moritani N, Lyons KM, Iida S, Takigawa M.
  • Journal Title: J Cell Biochem Volume: 115 Issue: 5 Pages: 854-865
  • DOI: 10.1002/jcb.24728
  • NAID: 120005468290
  • Peer Reviewed / Open Access
• [Journal Article] The regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarthritis models in vitro (2014)
  • Author(s): Tarek Abd El Kader, et al.
  • Journal Title: Bone Volume: 59 Pages: 180-188
  • DOI: 10.1016/j.bone.2013.11.010
  • Peer Reviewed / Open Access
• [Journal Article] Differential expression of vascular endothelial growth factor in high- and low- metastasis cell lines of salivary gland adenoid cystic carcinoma. (2014)
  • Author(s): Kondo, S., Mukudai, Y., Soga, D., Nishida, T., Takigawa, M. and Shirota, T.
  • Journal Title: Anticancer Res., 2014, in press. Volume: 34 Pages: 671-677
  • Peer Reviewed
• [Journal Article] CCN family member 2/connective tissue growth factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes (2013)
  • Author(s): Itoh, S., Hattori, T., Tomita, N., Aoyama, E., Yutani,Y., Yamashiro, T., Takigawa, M.
  • Journal Title: PLoS One Volume: 8 Issue: 8 Pages: e71156-e71156
  • DOI: 10.1371/journal.pone.0071156
  • NAID: 120005345531
  • Peer Reviewed
• [Journal Article] Regulation of CCN1 via the 3’-untranslated region. (2013)
  • Author(s): Nakagawa, Y., Minato, M., Sumiyoshi, K., Maeda, A., Hara, C., Murase, Y., Nishida, T., Kubota, S. and Takigawa, M.:
  • Journal Title: J. Cell Commun. Signal., 7, 207-217, 2013. Volume: 7 Issue: 3 Pages: 207-217
  • DOI: 10.1007/s12079-013-0202-x
  • Peer Reviewed
• [Journal Article] Cartilage–specific overexpression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth (2013)
  • Author(s): Tomita, N., Hattori, T., Ito, S., Aoyama, E., Yao, M., Yamashiro, T., Takigawa M.
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: e59226-e59226
  • DOI: 10.1371/journal.pone.0059226
  • NAID: 120005345532
  • Peer Reviewed
• [Journal Article] Impaired glycolytic metabolism causes chondrocyte hypertrophy-like changes via promotion of phospho-Smad1/5/8 translocation into nucleus. (2013)
  • Author(s): Nishida, T., Kubota, S., Aoyama, E. and Takigawa, M.
  • Journal Title: Osteoarthritis Cartilage, Volume: 21 Issue: 5 Pages: 770-709
  • DOI: 10.1016/j.joca.2013.01.013
  • Peer Reviewed
• [Journal Article] Novel chondrogenic and chondroprotective effects of the natural compound harmine. (2013)
  • Author(s): Hara, E. S., Ono, M., Kubota, S., Sonoyama, W., Oida, Y., Hattori, T., Nishida, T., Furumatsu, T., Ozaki, T., Takigawa, M. and Kuboki, T
  • Journal Title: Biochimie Volume: 95 Issue: 2 Pages: 347-381
  • DOI: 10.1016/j.biochi.2012.10.016
  • Peer Reviewed
• [Journal Article] Anti-fibrotic effect of CCN3 accompanied by altered gene expression profile of the CCN family. (2013)
  • Author(s): Abd El Kader, T., Kubota, S., Janune, D., Nishida, T., Hattori, T., Aoyama, E., Perbal, B., Kuboki, T. and Takigawa, M.
  • Journal Title: J. Cell Commun. Signal. Volume: 7 Issue: 1 Pages: 11-18
  • DOI: 10.1007/s12079-012-0180-4
  • Peer Reviewed
• [Journal Article] Novel role of miR-181a in cartilage metabolism. (2013)
  • Author(s): Sumiyoshi K, Kubota S, Ohgawara T, Kawata K, Abd El Kader T, Nishida T, Ikeda N, Shimo T, Yamashiro T, Takigawa M
  • Journal Title: J Cell Biochem Volume: in press Issue: 9 Pages: 2094-100
  • DOI: 10.1002/jcb.24556
  • Peer Reviewed
• [Journal Article] Roles of heterotypic CCN2/CTGF-CCN3/N0V and homotypic CCN2-CCN2interacti ons in expression of the differentiated phenotype ofchondrocytes. (2012)
  • Author(s): Hoshij ima M., et al.
  • Journal Title: FEBS J Volume: 279(19) Issue: 19 Pages: 3584-3597
  • DOI: 10.1111/j.1742-4658.2012.08717.x
  • Peer Reviewed
• [Journal Article] Role of low-density lipoprotein receptor related protein 1 (LRP1) in CCN2/connective tissue growth factor (CTGF) protein transport in chondrocytes. (2012)
  • Author(s): Kawata., K., Kubota, S., Eguchi, T., Aoyama, E., Moritani, N., Kondo, S., Nishida, T., Takigawa, M.
  • Journal Title: J Cell Sci Volume: 15 Pages: 2965-2972
  • DOI: 10.1242/jcs.101956
  • Peer Reviewed
• [Journal Article] Effect of CCN2 on FGF2-induced proliferation and MMP9 and MMP13 productions by chondrocytes (2011)
  • Author(s): Nishida T, et al
  • Journal Title: Endocrinology Volume: 152 Issue: 11 Pages: 4232-4241
  • DOI: 10.1210/en.2011-0234
  • Peer Reviewed
• [Journal Article] Novel effects of CCN3 that may direct the differentiation of chondrocytes (2011)
  • Author(s): Janune, D., et al
  • Journal Title: FEBS Lett. Volume: 585 Issue: 19 Pages: 3033-3040
  • DOI: 10.1016/j.febslet.2011.08.024
  • Peer Reviewed
• [Journal Article] Binding of glyceraldehyde-3-phosphate dehydrogenase to the cic-acting element of structure-anchored repression in Ccn2 mRNA. (2011)
  • Author(s): Kondo, S. et al.
  • Journal Title: Biochem. Biophys. Res. Commun., Volume: 405 Issue: 3 Pages: 382-387
  • DOI: 10.1016/j.bbrc.2011.01.034
  • Peer Reviewed
• [Journal Article] CCN family 2/ connective tissue growth factor (CCN2/CTGF) promotes osteoclastogenesis via induction of and interaction with dendritic cell-specific transmembrane protein (DC-STAMP). (2011)
  • Author(s): Nishida, T. et al.
  • Journal Title: J. Bone Miner. Res. Volume: 26 Issue: 2 Pages: 351-363
  • DOI: 10.1002/jbmr.222
  • Peer Reviewed
• [Journal Article] Role of low-density lipoprotein receptor related protein 1 (LRP1) in CCN2/connective tissue growth factor (CTGF) protein transport in chondrocytes. (2011)
  • Author(s): Kawata., K. et al.
  • Journal Title: J. Cell Sci. Volume: in press
  • Peer Reviewed
• [Presentation] 軟骨細胞における低出力超音波(LIPUS)とROCK阻害剤によるCCN2の相加的産生 (2014)
  • Author(s): 西田
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都
• [Presentation] 軟骨細胞のエネルギー代謝におけるCCN2の役割。 (2014)
  • Author(s): 前田 彩、久保田聡、川木晴美、河田かずみ、三宅由晃、服部高子、西田 崇、森谷徳文、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都
• [Presentation] Fibroblast growth factor-1が軟骨代謝に及ぼす多彩な影響。 (2014)
  • Author(s): Abd El Kdaer, Tarek、久保田聡、西田 崇、古松毅之、青山絵理子、窪木拓男、滝川正春
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都
• [Presentation] 軟骨細胞における低出力超音波(LIPUS)とROCK阻害剤によるCCN2の相加的産生。 (2014)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、Karen M Lyons、滝川正春
  • Organizer: 第27回日本軟骨代謝学会
  • Place of Presentation: 京都
• [Presentation] マウス筋芽細胞においてCCN2はBMP2による骨芽細胞様変化を抑制する (2013)
  • Author(s): 西田
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山
• [Presentation] 軟骨細胞におけるCCN2発現の低出力超音波(LIPUS)による誘導 (2013)
  • Author(s): 西田
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] CCN2 is up-regulated in cultured chondrocytes treated with low-intensity plused ultrasound (LIPUS) (2013)
  • Author(s): Nishida
  • Organizer: 2nd joint meeting of IBMS-JSBMR
  • Place of Presentation: Kobe, Japan
• [Presentation] 軟骨細胞におけるCCN2発現の低出力超音波(LIPUS)による誘導 (2013)
  • Author(s): 西田
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] メトロボミクスとインタラミクスが描き出すCCN2の新たな機能。 (2013)
  • Author(s): 久保田聡、前田 彩、西田 崇、滝川正春：
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Invited
• [Presentation] 軟骨細胞におけるCCN2発現及び産生量に与える低出力性超音波(LIPUS)の効果。 (2013)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、滝川正春
  • Organizer: 第55回歯科基礎医学会学術大会、サテライトシンポジウム4
  • Place of Presentation: 岡山
• [Presentation] CCN2は軟骨細胞のエネルギー代謝に重要である。 (2013)
  • Author(s): 前田 彩、久保田聡、川木晴美、河田かずみ、三宅由晃、服部高子、西田 崇、森谷徳文、飯田征二、滝川正春
  • Organizer: 第55回歯科基礎医学会学術大会、サテライトシンポジウム9
  • Place of Presentation: 岡山
• [Presentation] CCN2 inhibits BMP2-induced heterotopic ossification. (2013)
  • Author(s): Nishida, T.
  • Organizer: Okayama Univ.-Harvard Univ.-Helsinki Univ.-NUS- Calcified Tissue Joint Seminar
  • Place of Presentation: Okayama, Japan
• [Presentation] Roles of CCN2 and CCN3 in skeletogenesis. (2013)
  • Author(s): Takigawa, M., Kubota, S., Hattori, T., Nishida, T., Apyama, E., Tomita, N., Itoh, S., Janune, D., Hoshijima, M.
  • Organizer: Seventh International Workishop on the CCN Family of Genes
  • Place of Presentation: Nice, France
• [Presentation] The novel role of CCN2 as a regeratory factor in RANK/RANKL/OPG system. (2013)
  • Author(s): Aoyama, E., Kubota, S., Nishida, T., Kawaki, H., Takigawa, M.
  • Organizer: Seventh International Workishop on the CCN Family of Genes
  • Place of Presentation: Nice, France
• [Presentation] CCN2 enhances osteoclastogenesis via its direct bindings to RANK and OPG. (2013)
  • Author(s): Aoyama, E., Kubota, S., Nishida, T., Takigawa, M.
  • Organizer: 2nd joint meeting of IBMS-JSBMR,
  • Place of Presentation: Kobe, Japan
• [Presentation] CCN2 is up-regulated in cultured chondrocytes treated with low-intensity plused ultrasound (LIPUS). (2013)
  • Author(s): Nishida, T., Kubota, S., Aoyama, E., Yamanaka, N., Takigawa, M.
  • Organizer: 2nd joint meeting of IBMS-JSBMR,
  • Place of Presentation: Kobe, Japan
• [Presentation] Crucial role of CCN2 in energy metabolism in chondrocytes. (2013)
  • Author(s): Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Hattori, T., Nishida, T., Moritani, N., Kawaki, H., Lyons, K., Iida, S., Takigawa, M.
  • Organizer: 2nd joint meeting of IBMS-JSBMR,
  • Place of Presentation: Kobe, Japan
• [Presentation] Identification and characterization of chondrocytic fibroblast growth factor 1 as a molecular counterpart of CCN family member 2. (2013)
  • Author(s): Abd El Kader, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Furumatsu, T., Kuboki, T., Takigawa, M.
  • Organizer: 2nd joint meeting of IBMS-JSBMR,
  • Place of Presentation: Kobe, Japan
• [Presentation] Molecular mechanism of CCN2-induced osteoclastogenesis. (2013)
  • Author(s): Aoyama, E., Kubota, S., Nishida, T., Takigawa M.
  • Organizer: The 38th FEBS Congress
  • Place of Presentation: St. Petersburg, Russia
• [Presentation] 軟骨細胞のエネルギー代謝におけるCCN2の役割。 (2013)
  • Author(s): 前田 彩、久保田聡、三宅由晃、河田かずみ、西田 崇、服部高子、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第4回骨バイオサイエンス研究会
  • Place of Presentation: 岡山
• [Presentation] Essential role of CCN2 that supports the basal energy metabolism in chondrocytes． (2013)
  • Author(s): Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Hattori, T., Nishida, T., Norifumi Moritani, N., Kawaki, H., Lyons, K. M., Iida, S., Takigawa, M.
  • Organizer: 第86回日本生化学会大会
  • Place of Presentation: 横浜
• [Presentation] 炎症・組織再生を制御する CCN1 の 3’ 非翻訳領域を介した遺伝子発現調節。 (2013)
  • Author(s): 村瀬友里香、久保田聡、前田 彩、原 規子、住吉久美、西田 崇、佐々木朗、滝川正春
  • Organizer: 第 55 回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] Identification of Rab14 GTPase as a CCN2/CTGF binding protein and the role of this interaction in vesicle trafficking in chondrocytes. (2013)
  • Author(s): 星島光博, 服部高子, 青山絵理子, 西田崇, 山城隆, 滝川正春
  • Organizer: 第 55 回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] マウス筋芽細胞においてCCN2はBMP2による骨芽細胞様変化を抑制する。 (2013)
  • Author(s): 西田 崇、久保田聡、Janune Danilo、滝川正春
  • Organizer: 第 55 回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
• [Presentation] 軟骨細胞と変形性関節症モデルを用いたCCN2各モジュールの組織再生効果の評価 (2013)
  • Author(s): Abd El Kdaer, Tarek、久保田聡、西田 崇、服部高子、青山絵理子、Janune Danilo、窪木拓男、滝川正春
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山
• [Presentation] CCN3の抗線維化効果に伴うCCNファミリー遺伝子発現プロファイルの変化 (2013)
  • Author(s): Janune Danilo、Abd El Kdaer, Tarek、久保田聡、西田 崇、服部高子、青山絵理子、窪木拓男、滝川正春
  • Organizer: 第55回歯科基礎医学会
  • Place of Presentation: 岡山
• [Presentation] Anti-fibrotic role of CCN3 through regulation of CCN family genes. (2013)
  • Author(s): Abd El Kdaer, T., Kubota, S., Janune, D., Nishida, T., Hattori, T., Aoyama, E., Perbal, B., Takigawa, M.
  • Organizer: The third international symposium of medical-dental-pharmaceutical education and research in Okayama
  • Place of Presentation: Okayama, Japan
• [Presentation] Molecular mechanism of CCN2-induced osteoclastogenesis. (2013)
  • Author(s): Aoyama, E., Kubota, S., Nishida, T., Takigawa, M.
  • Organizer: The third international symposium of medical-dental-pharmaceutical education and research in Okayama
  • Place of Presentation: Okayama, Japan
• [Presentation] 炎症・組織再生を制御する CCN1 の 3’ 非翻訳領域を介した遺伝子発現調節。 (2013)
  • Author(s): 村瀬友里香、仲川洋介、湊 雅直、前田 彩、原 規子、住吉 久美、西田 崇、佐々木朗、久保田聡、滝川正春
  • Organizer: 第 34 回岡山歯学会学術集会
  • Place of Presentation: 岡山
• [Presentation] CCN2/CTGFとRab14 GTPaseの相互作用が軟骨細胞の小胞輸送に及ぼす役割 (2013)
  • Author(s): 星島光博, 服部高子, 青山絵理子, 西田 崇, 滝川正春
  • Organizer: 第 34 回岡山歯学会学術集会
  • Place of Presentation: 岡山
• [Presentation] CCN3の抗線維化効果に伴うCCNファミリー遺伝子発現プロファイルの変化。 (2013)
  • Author(s): Janune Danilo、Abd El Kdaer, Tarek、久保田聡、西田 崇、服部高子、青山絵理子、窪木拓男、滝川正春
  • Organizer: 第 34 回岡山歯学会学術集会
  • Place of Presentation: 岡山
• [Presentation] CCN2とRab14 GTPaseの相互作用が軟骨細胞の小胞輸送に及ぼす役割 (2013)
  • Author(s): 星島光博, 服部高子, 青山絵理子, 西田崇, 滝川正春
  • Organizer: 第36回 日本分子生物学会年会
  • Place of Presentation: 神戸
• [Presentation] 軟骨細胞におけるCCN2発現の低出力超音波(LIPUS)による誘導。 (2013)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、滝川正春
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
• [Presentation] 軟骨細胞におけるCCN2発現の低出力超音波(LIPUS)による誘導。 (2013)
  • Author(s): 西田 崇、久保田聡、青山絵理子、山中信康、滝川正春
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] Cartilage regenerative effects of CCN2 independent modules. (2013)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Kuboki, T., Takigawa, M.
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] Rab14 GTPaseのCCN2/CTGF結合因子としての同定と、これらの相互作用が軟骨細胞の小胞輸送に及ぼす役割。 (2013)
  • Author(s): 星島光博、服部高子、青山絵理子、西田 崇、山城 隆、滝川正春
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 軟骨細胞のエネルギー代謝を支えるCCN2/CTGF。 (2013)
  • Author(s): 前田 彩、久保田聡、三宅由晃、河田かずみ、西田 崇、服部高子、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第26回日本軟骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 乳酸共存下での細胞内ATP量の減少は軟骨細胞の肥大化を引き起こす (2012)
  • Author(s): 西田
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] 乳酸共存下での細胞内ATP量の減少は軟骨細胞の肥大化を引き起こす (2012)
  • Author(s): 西田
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] The CCN2-inducer Harmine promotes chondrogenesis and protection against TNFα-induced inflammation. (2012)
  • Author(s): Hara, E., Ono, M., Kubota, S., Sonoyama, W., Oida, Y., Hattori, T., Nishida, T., Furumatsu, T., Ozaki, T., Takigawa, M., Kuboki, T
  • Organizer: 90th General Session & Exhibition of the IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Effect of CCN2 independent modules on chondrocytic cells. (2012)
  • Author(s): Abd El Kader, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Kuboki, T., Takigawa, M.
  • Organizer: 90th General Session & Exhibition of the IADR
  • Place of Presentation: Iguacu falls, Brazil
• [Presentation] Effect of CCN2 on FGF2-induced proliferation of and MMP-9 and -13 productions by chondrocytes. (2012)
  • Author(s): Nishida, T., Kubota, S., Aoyama, E., Janune, D., Maeda, A., Takigawa, M.
  • Organizer: 22nd IUBMB-37th FEBS Congress
  • Place of Presentation: Sevilla, Spain
• [Presentation] Roles of the heterotypic CCN2-CCN3 and homotypic CCN2-CCN2 interactions in matrix synthesis in chondrocytes. (2012)
  • Author(s): Hoshijima, M., Hattori, T., Aoyama, E., Nishida, T., Yamashiro, T., Takigawa, M.
  • Organizer: 2012 The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] Roles of CCN2 in energy metabolism in chondrocytes. (2012)
  • Author(s): Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Nishida, T., Hattori, T., Moritani, N., Kawaki, H., Lyons, K.M., Iida, S., Takigawa, M.
  • Organizer: 2012 The American Society for Cell Biology Annual Meeting
  • Place of Presentation: San Francisco, California, USA
• [Presentation] CCN2とOPGの分子間相互作用による新たな破骨細胞分化制御機構。 (2012)
  • Author(s): 青山絵理子、久保田聡、西田 崇、滝川正春
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 軟骨細胞のエネルギー代謝におけるCCN2/CTGFの役割。 (2012)
  • Author(s): 前田 彩、久保田聡、三宅由晃、河田かずみ、西田 崇、服部高子、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
• [Presentation] 軟骨細胞の代謝システムにおけるCCN2の役割。 (2012)
  • Author(s): 前田 彩、久保田聡、三宅由晃、河田かずみ、服部高子、西田 崇、森谷徳文、川木晴美、Karen M Lyons、飯田征二、滝川正春
  • Organizer: 第33回岡山歯学会学術集会
  • Place of Presentation: 岡山
• [Presentation] 乳酸共存下での細胞内ATP量の減少は軟骨細胞の肥大化を引き起こす。 (2012)
  • Author(s): 西田 崇、久保田聡、青山絵理子、滝川正春
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] CCN2/CTGF欠損が軟骨細胞のエネルギー代謝に及ぼす影響。 (2012)
  • Author(s): 前田 彩、久保田聡、服部高子、西田 崇、飯田征二、滝川正春
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] CCN2-OPG間相互作用による破骨細胞形成新規制御機構の解明。 (2012)
  • Author(s): 青山絵理子、久保田聡、西田 崇、滝川正春
  • Organizer: 第54回歯科基礎医学会
  • Place of Presentation: 郡山
• [Presentation] Effect of CCN2 independent modules on chondrogenic and osteoblastic cells. (2012)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Kuboki, T., Takigawa, M.
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] 乳酸共存下での細胞内ATP量の減少は軟骨細胞の肥大化を引き起こす。 (2012)
  • Author(s): 西田 崇、久保田聡、高嶋壮一朗、滝川正春
  • Organizer: 第30回日本骨代謝学会
  • Place of Presentation: 東京
• [Presentation] CCN2 modules; Independent and combinational effect on chondrocytic cells. (2012)
  • Author(s): Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Kuboki, T., Takigawa, M.
  • Organizer: 第3回骨バイオサイエンス研究会
  • Place of Presentation: 岡山
• [Presentation] CCN2/CTGF-CCN3/NOVおよびCCN2-CCN2のダイマー形成が軟骨細胞の分化機能に及ぼす役割。 (2012)
  • Author(s): 星島光博 他
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
• [Presentation] Functional characterization of CCN3 that may direct the differentiation of chondrocytes. (2012)
  • Author(s): Janune, D. et al.
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
• [Presentation] 解糖経路の阻害は重篤な軟骨変性を引き起こす。 (2012)
  • Author(s): 西田 崇 他
  • Organizer: 第25回日本軟骨代謝学会
  • Place of Presentation: 名古屋
• [Presentation] CCN2/CTGFはBMP2による骨格筋内異所性骨化に協調的に作用する (2011)
  • Author(s): 西田
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
• [Presentation] CCN2/CTGFはBMP2による骨格筋内異所性骨化に協調的に作用する (2011)
  • Author(s): 西田
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] 骨格筋内異所性骨化におけるCCN2/CTGFの作用 (2011)
  • Author(s): 西田
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] CCN2 promotes osteoclastogenesis via induction of and interaction with DC-STAMP and via interaction with RANK and enhancing RANKL signaling. (2011)
  • Author(s): Nishida, T. et al.
  • Organizer: Gordon Conference on Bones and Teeth
  • Place of Presentation: Les Diablerets, Switzerland
• [Presentation] CCN2/CTGF promotes osteoclastogenesis via induction of and interaction with Dendritic cell-specific transmembrane protein (DC-STAMP). (2011)
  • Author(s): Nishida, T. et al.
  • Organizer: 36th FEBS Congress
  • Place of Presentation: Torino, Italy
• [Presentation] Roles of the heterotypic CCN2/CTGF-CCN3/NOV and homotypic CCN2-CCN2 interactions in matrix synthesis in chondrocytes. (2011)
  • Author(s): Hoshijima, M. et al.
  • Organizer: The 33th Annual Meeting of the American Society for Bone and Mineral Research
  • Place of Presentation: San Diego, California
• [Presentation] Effect of CCN2 on FGF2-induced proliferation and MMP9 and MMP13 productions by chondrocytes. (2011)
  • Author(s): Nishida, T. et al.
  • Organizer: Targets 2011: Novel targets for cancer and connective tissues diseases
  • Place of Presentation: Vancouver, BC, Canada
• [Presentation] Novel effects of CCN3 that may direct the differentiation of chondrocytes. (2011)
  • Author(s): Janune, D. et al.
  • Organizer: Targets 2011: Novel targets for cancer and connective tissues diseases
  • Place of Presentation: Vancouver, BC, Canada
• [Presentation] Characterization of the effect of CCN2 modules independently and in different combinations on chondrocytic cells. (2011)
  • Author(s): Abd El Kader, T. et al.
  • Organizer: Targets 2011: Novel targets for cancer and connective tissues diseases
  • Place of Presentation: Vancouver, BC, Canada
• [Presentation] CCN2 promotes osteoclastogenesis via induction of and interaction with DC-STAMP and by enhancing RANKL signaling via interaction with RANK and osteoprotegerin. (2011)
  • Author(s): Nishida, T. et al.
  • Organizer: Cell Signaling Networks 2011: the 13th IUBMB Conference, the 1st PABMB Conference and the 3rd Meetings of the Signal Transduction Branch & Oxidative Stress Branches of SMB.
  • Place of Presentation: Merida, Yucatan, Mexico
• [Presentation] RANK結合タンパク質CCN2/CTGFはRANK-RANKLシグナルを促進する。 (2011)
  • Author(s): 青山絵理子 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] CCN2/CTGFとCCN3/Novのヘテロダイマー、およびCCN2のホモダイマー形成が軟骨細胞の基質合成に及ぼす役割。 (2011)
  • Author(s): 星島光博 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 軟骨細胞分化を運命づける分子としてのCCN3の役割。 (2011)
  • Author(s): ジャヌネ ダニーロ 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] Evaluation of independent and combinational effect of CCN2 modulates on chondrocytic cells (2011)
  • Author(s): Abd El Kdaer, T. et al.
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] 骨格筋内異所性骨化におけるCCN2/CTGFの作用。 (2011)
  • Author(s): 西田 崇 他
  • Organizer: 第29回日本骨代謝学会
  • Place of Presentation: 大阪
• [Presentation] CCN2のRANK(receptor activator of nuclear kappa B)の結合とRANK-RANKLシグナル制御作用 (2011)
  • Author(s): 青山絵理子 他
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] CCN2/CTGFとCCN3/Novは、ヘテロダイマーを形成することにより、軟骨細胞の基質合成を厳密に制御する。 (2011)
  • Author(s): 星島光博 他
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] Glyceroaldehyde-3-phosphate dehydrogeneseはCCN2 mRNA結合タンパクである。 (2011)
  • Author(s): 近藤誠二 他
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] Evaluation of independent and combinational effect of each of CCN2 modulates on chondrocytic cells. (2011)
  • Author(s): Abd El Kdaer, T. et al.
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] Harmine, an inducedr of CCN2/CTGF, promotes chondrogenesis and protection against TNF-α-induced inflammatory condition. (2011)
  • Author(s): Hara, ES. et al.
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] Novel effects of CCN3 that may direct the differentiation of chondrocytes. (2011)
  • Author(s): Janune, D. et al.
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] CCN2/CTGFはBMP2による骨格筋内異所性骨化に協調的に作用する。 (2011)
  • Author(s): 西田 崇 他
  • Organizer: 第4回日本CCNファミリー研究会
  • Place of Presentation: 岡山
• [Presentation] Receptor activator of NF-κB (RANK) 結合タンパク質であるCCN2/CTGFのRANKL誘導性破骨細胞形成における機能。 (2011)
  • Author(s): 青山絵理子 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] Assessment of combinational effect of independent CCN2 modules on chondrocytic cells. (2011)
  • Author(s): Abd El Kdaer, T. et al.
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
• [Presentation] CCN2/CTGFとCCN3/Novのヘテロダイマー形成が軟骨細胞の基質合成に及ぼす役割。 (2011)
  • Author(s): 星島光博 他
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
• [Presentation] CCN2/CTGFはBMP2による骨格筋内異所性骨化に協調的に作用する。 (2011)
  • Author(s): 西田 崇 他
  • Organizer: 第53回歯科基礎医学会
  • Place of Presentation: 岐阜
• [Presentation] Receptor activator of NF-κB (RANK) 結合タンパク質であるCCN2/CTGFのRANKL誘導性破骨細胞形成における機能。 (2011)
  • Author(s): 青山絵理子 他
  • Organizer: 第32回岡山歯学会学術集会
  • Place of Presentation: 岡山
• [Remarks] 岡山大学歯学部口腔生化学分野ホームページ
  • URL: http://www.okayama-u.ac.jp/user/seika/index.html
• [Remarks] 岡山大学歯学部口腔生化学分野