| Project/Area Number |
23592732
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Functional basic dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
NISHIDA Takashi 岡山大学, 医歯(薬)学総合研究科, 助教 (30322233)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGAWA Masaharu 岡山大学, 大学院医歯薬学総合研究科, 教授 (20112063)
KUBOTA Satoshi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (90221936)
HATTORI Takako 岡山大学, 大学院医歯薬学総合研究科, 助教 (00228488)
AOYAMA Eriko 岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650)
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| Project Period (FY) |
2011 – 2013
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2012: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | CCN2/CTGF / C2C12細胞 / 骨格筋分化 / 異所性骨化 / BMP2 / Ccn2欠損筋芽細胞 / 筋芽細胞の増殖 / 筋芽細胞の分化 / 筋肉内異所性骨化 / マウス筋芽細胞 / CCN2 / CCNファミリー / 筋管形成 / 骨芽細胞様変化 / CCN2/結合組織成長因子 / 炎症性シグナル |
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| Research Abstract |
The protein production of CCN2 (also known as Connective tissue growth factor) was increased in mouse myoblastic cell line C2C12 by treatment with tumor necrosis factor-a, which is produced upon inflammation. CCN2 promoted cell proliferation and the protein production of MyoD in C2C12 cells. Consistent with these findings, in vivo analyses with Ccn2-deficient skeletal muscle showed the decreased PCNA staining and muscle hypoplasia. Furthermore, bone morphogenetic protein (BMP)2-induced Runx2 and Osterix gene expression levels were significantly decreased in C2C12 cells co-treated with CCN2.
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