The study on the expression of monoamine transporters after onset of neuropathic pain

• Project/Area Number: 23592733
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Functional basic dentistry
• Research Institution: Okayama University
• Principal Investigator: SOGAWA Chiharu 岡山大学, 医歯(薬)学総合研究科, 助教 (10253022)
• Co-Investigator(Kenkyū-buntansha): KITAYAMA Shigeo 岡山大学, 大学院医歯薬学総合研究科, 教授 (80177873) ; SOGAWA Norio 岡山大学, 大学院医歯薬学総合研究科, 准教 (30236153) ; MORITA Katsuya 広島文化学園大学, 看護学部看護学科, 教授 (10116684)
• Project Period (FY): 2011 – 2013
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000); Fiscal Year 2013: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2012: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 神経因性疼痛 / トランスポーター / モノアミン / 抗うつ薬 / GABA

Research Abstract

Neuropathic pain is defined with for a chronic or persistent pain to be generated by a pathophysiological alteration on the peripheral and/or central nervous system (CNS). Antidepressants are known as the inhibitors of monoamine neurotransmitter transporters (MATs) are therapeutically useful ligands for the treatment of neuropathic pain. However, they do not sometimes provide enough effects. We focused the change of MATs expression level in the CNS after the onset of the neuropathic pain. In this study, MATs mRNA level decreased in the brain stem after onset of the pain in limited neuropathic pain model. We suggested that the change of MAT expression in the CNS associated with therapeutic efficacy of antidepressants for the neuropathic pain.

Research Products

• [Journal Article] Antiallodynic action of 1-(3-(9H-Carbazol-9-yl)-1-propyl)-4-(2-methyoxyphenyl)-4-piperidinol (NNC05-2090), a betaine/GABA transporter inhibitor (2014)
  • Author(s): Ayako Jinzenji, Chiharu Sogawa, Takuya Miyawaki, Xuefang Wen, Dan Yi, Kazumi Ohyama, Shigeo Kitayama, Norio Sogawa, Katsuya Morita
  • Journal Title: Journal of Pharmacological Science Volume: in press Pages: 0-0
  • NAID: 130004438625
  • Peer Reviewed
• [Journal Article] Protective effect of cepharanthin on cisplatin-induced renal toxicity through metallothionein expression (2013)
  • Author(s): Sogawa, N., Hazehara, Y., Kunitomo, K. Morita, Y., Bupsang, Y., Ohyama, K., Sogawa, C. and Kitayama, S
  • Journal Title: Life Sci Volume: 92 Issue: 12 Pages: 727-732
  • DOI: 10.1016/j.lfs.2013.01.031
  • Peer Reviewed
• [Journal Article] Age-dependent changes in the susceptibility to thiopental anesthesia in mice: analysis of the relationship to the functional expression of GABA transporter (2012)
  • Author(s): Norio Sogawa, Yuri Hazehara, Muneyoshi Kunitomo, Yuya Morita, Bupsang Yoo, Kazumi Ohyama, Chiharu Sogawa and Shigeo Kitayama
  • Journal Title: Pharmacology, Biochemistry and Behavior Volume: 103 Issue: 2 Pages: 267-272
  • DOI: 10.1016/j.pbb.2012.08.027
  • Peer Reviewed
• [Journal Article] Inhibitory action of antidepressants on mouse betaine/GABA Transporter (BGT1) heterologously expressed in cell cultures (2012)
  • Author(s): Gerile, Sogawa, C., Ohyama, K., Masuko, T., Kusama, T., Morita, K., Sogawa, N. and Kitayama, S
  • Journal Title: Int. J. Mol. Sci Volume: 13 Issue: 3 Pages: 2578-89
  • DOI: 10.3390/ijms13032578
  • Peer Reviewed
• [Journal Article] Inhibitory action of antidepressants on mouse betaine/GABA transporter (BGT1) heterologously expressed in cell cultures. (2012)
  • Author(s): Gerile, C.Sogawa, K. Ohyama, T. Masuko, T. Kusama,et al.
  • Journal Title: International Journal of Molecular Sciences Volume: 13 Pages: 2578-2589
  • Peer Reviewed
• [Journal Article] (2011)
  • Author(s): Sogawa, C., Sogawa, N., Ohyama, K., Kikura-Hanajiri, R., Goda, Y., Sora, I. and Kitayama, S
  • Journal Title: Curr Neuropharmacol Volume: 9(1) Issue: 1 Pages: 58-62
  • DOI: 10.2174/157015911795017425
  • Peer Reviewed
• [Journal Article] Methylone and Monoamine Transporters: Correlation with Toxicity (2011)
  • Author(s): C. Sogawa, N. Sogawa, K. Ohyama, et al.
  • Journal Title: Current Neuropharmacology Volume: 9 Pages: 58-62
  • Peer Reviewed
• [Journal Article] Antiallodynic action of 1-(3-(9H-Carbazol-9-yl)-1-propyl)-4-(2-methyo xyphenyl)-4-piperidinol (NNC05-2090), a betaine/GABA transporter inhibitor
  • Author(s): Jinzenji, A., Sogawa, C., Miyawaki, T., Wen, X., Yi, D., Ohyama, K., Kitayama, S., Sogawa, N., and Morita, K
  • Journal Title: J. Pharmacol., Sci Volume: in press
  • NAID: 130004438625
  • Peer Reviewed
• [Presentation] Wen, X., Sogawa, C., Ohyama, K., Jinzenji, A., Miyawaki, T., and Sogawa, N.: Coexpression of H3R decrease NET uptake activity and membrane expression in CHO cells stably express NET.第87回日本薬理学会年会、2014年3月21日、仙台。 (2014)
  • Author(s): Wen, X., Sogawa, C., Ohyama, K., Jinzenji, A., Miyawaki, T., and Sogawa, N
  • Organizer: NET.第87回日本薬理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2014-03-21
• [Presentation] Coexpression of H3R decrease NET uptake activity and membrane expression in CHO cells stably express NET (2014)
  • Author(s): Xue-fang Wen, Chiharu Sogawa, Kazumi Ohyama, Ayako Jinzenji, Takuya Miyawaki, Norio Sogawa
  • Organizer: 第87回日本薬理学会年会
  • Place of Presentation: 仙台市
• [Presentation] Regulation of noradrenaline transporter gene expression by nicotine. Neuroscience 2013 Annual Meeting (2013)
  • Author(s): Sogawa,N., Sogawa, C., Ohyama, K., Wen, X., Yi, D. and Kitayama, S
  • Organizer: Neuroscience 2013 Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-10
• [Presentation] Anti-allodynic action of NNC 05-2090, a betaine/GABA transporter inhibitor (2013)
  • Author(s): Jinzenji, A., Sogawa, C., Miyawaki, T., Morita, K., Kitayama, S. and Sogawa, N
  • Organizer: Neuroscience 2013 Annual Meeting
  • Place of Presentation: San Diego, USA
  • Year and Date: 2013-11-09
• [Presentation] 新規障害性疼痛に対するGABAトランスポーター阻害薬の効果 (2013)
  • Author(s): 秦泉寺紋子、十川千春、宮脇卓也、森田克也、十川紀夫、北山滋雄
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山
  • Year and Date: 2013-09-21
• [Presentation] Characterization of human noradrenaline transporter gene at 5'-flanking region (2013)
  • Author(s): Sogawa, C., Ohyama, K., Sogawa, N. and Kitayama, S
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-23
• [Presentation] Post- transcriptional regulation of noradrenaline transporter by nicotine (2013)
  • Author(s): Ohyama, K., Sogawa, C., Wen, X., Morita, K., Sogawa, N. and Kitayama, S
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2013-03-23
• [Presentation] 神経障害性疼痛に対するGABAトランスポーター阻害薬の鎮痛効果 (2013)
  • Author(s): 秦泉寺紋子, 十川千春，宮脇卓也，森田克也，十川紀夫，北山滋雄
  • Organizer: 第55回歯科基礎医学会学術大会
  • Place of Presentation: 岡山市
• [Presentation] Regulation of noradrenaline transporter gene expression by nicotine. (2013)
  • Author(s): Norio Sogawa, Chiharu Sogawa, Kazumi Ohyama, Xue-fang Wen, Dan Yi, Shigeo Kitayama
  • Organizer: Neuroscience2013 Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Anti-allodynic action of NNC05-2090, a betaine/GABA transporter inhibitor. (2013)
  • Author(s): Ayako Jinzenji, Chiharu Sogawa, Takuya Miyawaki, Katsuya Morita, Shigeo Kitayama, Norio Sogawa
  • Organizer: Neuroscience2013 Annual Meeting
  • Place of Presentation: San Diego
• [Presentation] Characterization of human noradrenaline transporter gene at 5’-flanking region (2013)
  • Author(s): Chiharu Sogawa, Kazumi Ohyama, Norio Sogawa, Shigeo Kitayama
  • Organizer: 第86回日本薬理学会年会
  • Place of Presentation: 福岡市
• [Presentation] ヒトノルアドレナリントランスポーター遺伝子5'プロモーター領域の解析 (2012)
  • Author(s): 北山滋雄、十川千春、大山和美、十川紀夫
  • Organizer: 第122回日本薬理学会近畿部会
  • Place of Presentation: 豊中
  • Year and Date: 2012-11-16
• [Presentation] ヒトノルアドレナリントランスポーター遺伝子5’プロモーター領域の解析 (2012)
  • Author(s): 北山滋雄，十川千春，大山和美，十川紀夫
  • Organizer: 第122回日本薬理学会近畿部会
  • Place of Presentation: 豊中市
• [Book] Site-directed Mutagenesis for the Identification of Ligand-binding Sites on Na+/Cl--dependent Neurotransmitter Transporters. Protein Purification and Analysis III - Methods and Applications (2014)
  • Author(s): Sogawa, C., Sogawa, N. and Kitayama, S.
  • Publisher: (ISBN:=iConcept Press)
  • URL: http://www.iconceptpress.com/books/protein-purification-and-analysis-iii--methods-and-applications/
• [Book] Protein Purification and Analysis II - Methods and Applications (2013)
  • Author(s): Sogawa C, Sogawa N, Kitayama S
  • Publisher: iConcept Press Ltd
• [Book] Immunocytochemical approaches to the identification of membrane topology of the Na + /Cl - -dependent neurotransmitter transporters. Applications of immunocytochemistry (2012)
  • Author(s): Sogawa, C., Sogawa, N. and Kitayama, S
  • Publisher: INTECH
• [Book] Applications of immunocytochemistry （Chapter6) (2012)
  • Author(s): C. Sogawa, N. Sogawa, S. Kitayama
  • Total Pages: 320
  • Publisher: INTECH